Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)

Kalkidan Shiferaw Abebe

doi:doi:10.11648/j.abb.20190701.11

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Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)

Kalkidan Shiferaw Abebe

Published in Advances in Bioscience and Bioengineering (Volume 7, Issue 1)

Received: 12 April 2019 Accepted: 3 June 2019 Published: 19 June 2019

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Abstract

Genotype by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable genotyping of large numbers of individuals at a large number of SNP markers. It is relatively straightforward, robust, and cost-effective method to reduce problems in crop caused by a large genome size, reduced representation libraries are produced using a restriction enzyme that targets genomic regions while multiplexing with barcodes reduces the cost for individual sample. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra- throughput sequences to revolutionize plant genotyping and breeding. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, QTL mapping, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs. GBS will have broad application in genomics-assisted plant breeding programs.

Published in	Advances in Bioscience and Bioengineering (Volume 7, Issue 1)
DOI	10.11648/j.abb.20190701.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Single Nucleotide Polymorphism (SNP), Next Generation Sequencing (NGS), Reduced Representation Library (RRL), Marker Assisted Selection (MAS)

References

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Kalkidan Shiferaw Abebe. (2019). Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Advances in Bioscience and Bioengineering, 7(1), 1-7. https://doi.org/10.11648/j.abb.20190701.11

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Kalkidan Shiferaw Abebe. Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Adv. BioSci. Bioeng. 2019, 7(1), 1-7. doi: 10.11648/j.abb.20190701.11

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Kalkidan Shiferaw Abebe. Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Adv BioSci Bioeng. 2019;7(1):1-7. doi: 10.11648/j.abb.20190701.11

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@article{10.11648/j.abb.20190701.11,
  author = {Kalkidan Shiferaw Abebe},
  title = {Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.abb.20190701.11},
  url = {https://doi.org/10.11648/j.abb.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190701.11},
  abstract = {Genotype by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable genotyping of large numbers of individuals at a large number of SNP markers. It is relatively straightforward, robust, and cost-effective method to reduce problems in crop caused by a large genome size, reduced representation libraries are produced using a restriction enzyme that targets genomic regions while multiplexing with barcodes reduces the cost for individual sample. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra- throughput sequences to revolutionize plant genotyping and breeding. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, QTL mapping, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs. GBS will have broad application in genomics-assisted plant breeding programs.},
 year = {2019}
}

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TY  - JOUR
T1  - Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)
AU  - Kalkidan Shiferaw Abebe
Y1  - 2019/06/19
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20190701.11
DO  - 10.11648/j.abb.20190701.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
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PB  - Science Publishing Group
SN  - 2330-4162
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AB  - Genotype by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable genotyping of large numbers of individuals at a large number of SNP markers. It is relatively straightforward, robust, and cost-effective method to reduce problems in crop caused by a large genome size, reduced representation libraries are produced using a restriction enzyme that targets genomic regions while multiplexing with barcodes reduces the cost for individual sample. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra- throughput sequences to revolutionize plant genotyping and breeding. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, QTL mapping, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs. GBS will have broad application in genomics-assisted plant breeding programs.
VL  - 7
IS  - 1
ER  -

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Kalkidan Shiferaw Abebe

Department of Plant Sciences, Gambella University, Gambella, Ethiopia

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Kalkidan Shiferaw Abebe. (2019). Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Advances in Bioscience and Bioengineering, 7(1), 1-7. https://doi.org/10.11648/j.abb.20190701.11

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Kalkidan Shiferaw Abebe. Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Adv. BioSci. Bioeng. 2019, 7(1), 1-7. doi: 10.11648/j.abb.20190701.11

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AMA Style

Kalkidan Shiferaw Abebe. Genotype by Sequencing Method and Its Application for Crop Improvement (A Review). Adv BioSci Bioeng. 2019;7(1):1-7. doi: 10.11648/j.abb.20190701.11

Copy | Download

@article{10.11648/j.abb.20190701.11,
  author = {Kalkidan Shiferaw Abebe},
  title = {Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.abb.20190701.11},
  url = {https://doi.org/10.11648/j.abb.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190701.11},
  abstract = {Genotype by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable genotyping of large numbers of individuals at a large number of SNP markers. It is relatively straightforward, robust, and cost-effective method to reduce problems in crop caused by a large genome size, reduced representation libraries are produced using a restriction enzyme that targets genomic regions while multiplexing with barcodes reduces the cost for individual sample. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra- throughput sequences to revolutionize plant genotyping and breeding. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, QTL mapping, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs. GBS will have broad application in genomics-assisted plant breeding programs.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Genotype by Sequencing Method and Its Application for Crop Improvement (A Review)
AU  - Kalkidan Shiferaw Abebe
Y1  - 2019/06/19
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20190701.11
DO  - 10.11648/j.abb.20190701.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20190701.11
AB  - Genotype by sequencing (GBS) is a next generation sequencing based method that takes advantage of reduced representation to enable genotyping of large numbers of individuals at a large number of SNP markers. It is relatively straightforward, robust, and cost-effective method to reduce problems in crop caused by a large genome size, reduced representation libraries are produced using a restriction enzyme that targets genomic regions while multiplexing with barcodes reduces the cost for individual sample. Several types of molecular markers, such as single nucleotide polymorphism (SNP), have been identified and effectively used in plant breeding. The application of next-generation sequencing (NGS) technologies has led to remarkable advances in whole genome sequencing, which provides ultra- throughput sequences to revolutionize plant genotyping and breeding. The GBS approach includes the digestion of genomic DNA with restriction enzymes followed by the ligation of barcode adapter, PCR amplification and sequencing of the amplified DNA pool on a single lane of flow cells. GBS has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, QTL mapping, genetic linkage analysis, molecular marker discovery and genomic selection under a large scale of plant breeding programs. GBS will have broad application in genomics-assisted plant breeding programs.
VL  - 7
IS  - 1
ER  -

Copy | Download