Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State
American Journal of Bioscience and Bioengineering
Volume 1, Issue 6, December 2013, Pages: 75-82
Received: Sep. 7, 2013; Published: Oct. 20, 2013
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Nworie O., Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State
Nnachi A. U., Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ukaegbu C. O, Department of Microbiology, Faculty of Natural Sciences, University of Jos, Plateau State
Alo M. N., Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ekuma U. O., Department of Applied Microbiology, Faculty of Biological Science, Ebonyi State University, Abakaliki, Ebonyi State
Ogueji E. O., Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State
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Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.
Antibiotics, Plasmid, S. aureus, P. aeruginosa, E. coli, Wound Infection, Abakaliki
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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., Ogueji E. O., Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State, American Journal of Bioscience and Bioengineering. Vol. 1, No. 6, 2013, pp. 75-82. doi: 10.11648/
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