Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites
American Journal of Bioscience and Bioengineering
Volume 2, Issue 2, April 2014, Pages: 33-36
Received: Mar. 13, 2014; Accepted: Jul. 17, 2014; Published: Jul. 30, 2014
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Authors
Enedina Jiménez-Cardoso, Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Apolinar Cano-Estrada, Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Adrian-Cortes Campos, Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
Leticia Eligio-García, Laboratory of parasitology, children hospital of Mexico, Dr. Marquez 162. Colonia Doctores, Cuauhtémoc. Mexico DF
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Abstract
The purpose of this study was to establish the optimal conditions to performance a multiple Polymerase Chain Reaction (multiplex) to detect the presence of emerging parasites Cryptosporidium spp., Mycrosporidium spp., Isospora belli, and Cyclospora spp. simultaneously, by targeting the 16S ribosomal gene in patients immunocompromised. After an exhaustive genetic analysis to obtain different primers sequences we tested the conditions of reagents concentration, time and temperature of reaction. The results showed the optimal conditions to make an efficient, specific, and reliable diagnostic method, performed in a simultaneous molecular reaction, to identify the presence of emerging parasites in faeces of patients susceptible to different infectious due to an immunocompromised status caused by diseases and chemotherapy to treat them. This method will help to decrease risk of complications and negative effects on life quality of ill patients.
Keywords
Cryptosporidium, Microsporidium, Isospora, Belli, Cyclospora, Multiplex, Immunocompromised, Emerging, Parasites
To cite this article
Enedina Jiménez-Cardoso, Apolinar Cano-Estrada, Adrian-Cortes Campos, Leticia Eligio-García, Multiple Polymerase Chain Reaction for Microsporidium spp, Cryptosporidium spp, Isospora Belli and Cyclospora Cayetanensis Parasites, American Journal of Bioscience and Bioengineering. Vol. 2, No. 2, 2014, pp. 33-36. doi: 10.11648/j.bio.20140202.12
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