Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology
American Journal of Bioscience and Bioengineering
Volume 2, Issue 6, December 2014, Pages: 72-77
Received: Dec. 18, 2014; Accepted: Jan. 5, 2015; Published: Jan. 14, 2015
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Authors
Tamara A. Ignatyeva, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Victor N. Voyevodin, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Anatoly N. Goltsev, Institute for Problems of Cryobiology and Cryomedicine NASU, 61015 Kharkov, Ukraine
Victoria V. Kiroshka, Institute for Problems of Cryobiology and Cryomedicine NASU, 61015 Kharkov, Ukraine
Alexander M. Bovda, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Valery V. Kalynovskii, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Alexey N. Velikodny, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Peter A. Kutsenko, National Scientific Center “Kharkov Institute of Physics and Technology”, 61108 Kharkov, Ukraine
Vladimir Golub, Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine
Yuri Dzhedzheria, Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine
Irina Sharai, Institute of Magnetism NASU and MESU, 03142 Kiev, Ukraine
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Abstract
Elastic hard magnetic materials based resin-bond magnets with the determined space configuration of the magnetic field required for a three-dimensional cell growth which is essential for the tissue engineering have been produced. Technical tests of the samples as well as the theoretical study of the distribution of stray fields produced by ferromagnetic particles correspondingly distributed in the film have been carried out. In vitro еxperimental investigations of the gradient magnetic field influence on a cell differentiation on transplanted epithelial-like kidney cells culture of a pig embryo has been carried out. It has been shown that the adhesion, morphology and proliferation rate of the cells is determined not only by the magnetic field value but also by its gradient direction. It has been established that the cell adhesion efficiency is the highest when the magnetic field gradient is directed from the Petri dish bottom to the air-culture medium interface. The obtained results prove the possibility of an implementation of new gradient magnetic fields based methods in biotechnology and in particular in tissue engineering.
Keywords
Resin-Bond Magnets, Cell Culture, Magnetic Field, Gradient Magnetic Field, Adhesion, Proliferation
To cite this article
Tamara A. Ignatyeva, Victor N. Voyevodin, Anatoly N. Goltsev, Victoria V. Kiroshka, Alexander M. Bovda, Valery V. Kalynovskii, Alexey N. Velikodny, Peter A. Kutsenko, Vladimir Golub, Yuri Dzhedzheria, Irina Sharai, Perspectives of Constant Gradient Magnetic Fields Applications in Biotechnology, American Journal of Bioscience and Bioengineering. Vol. 2, No. 6, 2014, pp. 72-77. doi: 10.11648/j.bio.20140206.11
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