Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria
American Journal of Bioscience and Bioengineering
Volume 3, Issue 5, October 2015, Pages: 40-46
Received: Aug. 31, 2015; Accepted: Sep. 13, 2015; Published: Sep. 26, 2015
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Majid Hussein Al-Jailawi, Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
Albab Fawaz Al-Faraas, Department of Biotechnology, College of Science, Al-Nahrain University, Baghdad, Iraq
Abdelghani Ibrahem Yahia, Department of Molecular & Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
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This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.
Dibenzothiophene, Biodesulfurization, Isolation, P. aeruginosa, GC/MS
To cite this article
Majid Hussein Al-Jailawi, Albab Fawaz Al-Faraas, Abdelghani Ibrahem Yahia, Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria, American Journal of Bioscience and Bioengineering. Vol. 3, No. 5, 2015, pp. 40-46. doi: 10.11648/
Akbar, A. H., 2008. Characterization of Some Benzothiophene and Dibenzothiophene Utilizing Bacteria. M. sc. Thesis. College of graduate studies- Arabian Gulf University.
Al-Hassar, Z. A., 2010. Biodegradation and Biodesulfuruzation Substrate Spectrum for Dibenzothiophene-Desulfurizing Bacteria. M. sc. Thesis. College of graduate studies- Arabian Gulf University.
Alves, L., Paixão, S. M., 2011. Toxicity Evaluation of 2-Hydroxybiphenyl and Other Compounds Involved in Studies of Fossil Fuels Biodesulphurisation. J. Bioresour. Technol., 102: 9162–9166.
Chen, H., Cai, YB., Zhang, W. J., Li, W., 2009. Methoxylation pathway in biodesulfurization of model organosulfur compounds with Mycobacterium sp. J. Bioresour. Technol.; 100: 2085–2087.
Gao, L. D., Tang, Y., Xue, Q. S., Liu, Y., Lu, Y., 2009. Hydrotalcite-like compounds derived Cu Zn Al oxide catalysts for aerobic oxidative removal of gasoline-range organosulfur compounds. Energy Fuels. 23, 624–630.
Gupta, N., Roychoudhury, P. K., Deb, J. K., 2005. Biotechnology of desulfurization of diesel: Prospects and challenges. Appl. Microbiol. Biotechnol. 66: 356–366.
Holt, J. G., Krieg, N. R., Sneath, H. A., Staley, J. T., Williams, S. T., 1994. Bergys manual of determinative bacteriology. 9th ed. Williams and Wilkins, USA.
Ichinose, H., Wariishi, H., Tanaka, H., 1999. Bioconversion of recalcitrant 4-methyldibenzothiophene to water-extractable products using lignin-degrading basidiomycete Coriolus versicolor. Biotechnol. Prog. 15: 706-714.
Kilbane, J. J., 2006. Microbial biocatalyst development to upgrade fossil fuels. Curr Opin Biotechnol 17, 305–314.
Konishi, J., Ishii, Y., Onaka, T., Okumura, K., Suzuki, M., 1997. Thermophilicarbon-sulfur-bond-targeted biode-sulfurization. Appl. Environ. Microbiol. 63 (8): 3164-9.
Liu, H., Yu, J., Bao, X., 2007. The State-of-the-art and future perspectives of world petroleum refining technology [J]. The Chinese Journal of Process Engineering. 7 (1): 176 -185.
Ma, C., Dai, B., Xu, C., Liu, P., Qi, L., Ban, L., 2013. Deep oxidative desulfurization of model fuel via dielectric barrier discharge plasma oxidation using MnO2 catalysts and combination of ionic liquid extraction. Catal. Today. 211, 84–89.
Mandal, A. J., Sarma, P. M., Singh, B., Jeyaseelan, C. P., Channashettar, V. A., 2012. Bioremediation: An environment friendly, sustainable biotechnological solution for remediation of petroleum hydrocarbon contaminated waste. ARPN Journal of Science and Technology 2 (Special Issue): 1-12.
Mohebali, G., Ball, A. S., 2008. Biocatalytic desulfurization (BDS) of petrodiesel fuels. Microbiology 154:2169–2183.
Mohebali, G., Ball, A., Rasekh, B., Kaytash, A., 2007. Biodesulfurization potential of a newly isolated bacterium, Gordonia alkanivorans RIPI190A. Enzyme and Microbial Technol., 40: 578-584.
Monticello, D. J., 2000. Biodesulfurization and the upgrading of petroleum distillates. Current Opinion in Biotechnology, 11, 540-546.
Oldfield, C., Pogrebinsky, O., Simmonds, J., Olson, E. S., Kulpa, C., 1997. Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968). Microbiology 143: 2961–2973.
Rambosek, J., Piddington, C. S., Kovacevich, B. R., Young, K. D., Denome S. A., 1994. Recombinant DNA encoding a desulfurization biocatalyst. U.S. Patent 5356801.
Rhee, S. K., Chang, J. H., Chang, Y. K., Chang, H. N., 1998. Desulfurization of Dibenzothiophene and Diesel Oil by Newly Isolated Gordona strain, CYKS1. Applied and Environmental Micobiol., 64 : 2327-2331.
Smart, K. F., Aggio, R. B. M., Van Houtte, J. R., Villas-Boas, S. G., 2010. Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography-mass spectrometry. Nat. Protoc. 5, 1709–1729.
Xu, P., Feng, J., Yu, B., Li, F., Ma, C., 2009. Recent developments in biodesulfurization of fossil fuels. Adv Biochem Eng Biotechnol 113:255–274.
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