Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.
Azab Elsayed Azab,
Mohamed Omar Albasha,
Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs, American Journal of Bioscience and Bioengineering.
Vol. 3, No. 5,
2015, pp. 80-86.
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