Increased Cell Proliferation by Various Doses of Xenoestrogen in MFC-7 Cell Through Tyrosine Phosphatase Shp2
American Journal of Bioscience and Bioengineering
Volume 3, Issue 6, December 2015, Pages: 178-182
Received: Dec. 23, 2015; Published: Dec. 23, 2015
Views 3921      Downloads 90
Authors
Wenjie Liu, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China
Qinyuan Wang, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China
Zhaokai Wang, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China
Defeng Wu, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China
Wenhua Liu, Third People's Hospital of Pingdu, Pingdu, China
Juan Wang, Third People's Hospital of Pingdu, Pingdu, China
Article Tools
Follow on us
Abstract
Our previous study demonstrated that tyrosine phosphatase Shp2 mediates the estrogen biological action in breast cancer. However, consumer research suggests that the Xenoestrogens exposure in breast tumorigenesisis more important. The present study examined the consequences of different concentrations xenoestrogens exposure to Bisphenol A (BPA) and Nonyl Phenol (NP) on cell proliferation and shp2 expression in MCF-7 cell. Phps1 (2x10-8 M), BPA (10-12, 10-11, 10-10, 10-9, 10-8, 10-7, 10-6 M), N P (10-12, 10-11, 10-10, 10-9, 10-8, 10-7, 10-6 M) and was administered in MCF-7 cell, respectively. Results showed the exposure of various concentrations xenoestrogens significantly elevated cell proliferation (P<0.05). Proliferation of MCF-7 cells was down regulated by Phps1 (a Shp2 inhibitor) when cells were treated for 1 h. In addition, the tyrosine phosphatase Shp2 levels were higher in BPA- and NP-exposed indicating that Shp2 plays a crutial role for Xenoestrogens exposure facilitating breast tumorigenesis properties.
Keywords
Xenoestrogens, Cellproliferation, Shp2, MCF-7 Cell
To cite this article
Wenjie Liu, Qinyuan Wang, Zhaokai Wang, Defeng Wu, Wenhua Liu, Juan Wang, Increased Cell Proliferation by Various Doses of Xenoestrogen in MFC-7 Cell Through Tyrosine Phosphatase Shp2, American Journal of Bioscience and Bioengineering. Vol. 3, No. 6, 2015, pp. 178-182. doi: 10.11648/j.bio.20150306.16
References
[1]
Soto AM, Vandenberg LN, Maffini MV, Sonnenschein C: Does breast cancer start in the womb? Basic Clin Pharmacol 2008, 102(2): 125-133.
[2]
Pupo M, Pisano A, Lappano R, Santolla MF, De Francesco EM, Abonante S, Rosano C, Maggiolini M: Bisphenol A induces gene expression changes and proliferative effects through GPER in breast cancer cells and cancer-associated fibroblasts. Environ Health Perspect 2012, 120(8): 1177-1182.
[3]
Zhang W, Fang Y, Shi X, Zhang M, Wang X, Tan Y: Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells. Molecular medicine reports 2012, 5(1): 41-47.
[4]
Jenkins S, Wang J, Eltoum I, Desmond R, Lamartiniere CA: Chronic oral exposure to bisphenol A results in a nonmonotonic dose response in mammary carcinogenesis and metastasis in MMTV-erbB2 mice. Environ Health Perspect 2011, 119(11): 1604-1609.
[5]
Jenkins S, Raghuraman N, Eltoum I, Carpenter M, Russo J, Lamartiniere CA: Oral exposure to bisphenol a increases dimethylbenzanthracene-induced mammary cancer in rats. Environ Health Perspect 2009, 117(6): 910-915.
[6]
Tharp AP, Maffini MV, Hunt PA, VandeVoort CA, Sonnenschein C, Soto AM: Bisphenol A alters the development of the rhesus monkey mammary gland. Proceedings of the National Academy of Sciences of the United States of America 2012, 109(21): 8190-8195.
[7]
Hunt PA, Lawson C, Gieske M, Murdoch B, Smith H, Marre A, Hassold T, VandeVoort CA: Bisphenol A alters early oogenesis and follicle formation in the fetal ovary of the rhesus monkey. Proceedings of the National Academy of Sciences of the United States of America 2012, 109(43): 17525-17530.
[8]
Chen CL, Chiang TH, Tseng PC, Wang YC, Lin CF: Loss of PTEN causes SHP2 activation, making lung cancer cells unresponsive to IFN-gamma. Biochem Biophys Res Commun 2015, 466(3): 578-584.
[9]
Park MA, Hwang KA, Lee HR, Yi BR, Choi KC: Cell Growth of BG-1 Ovarian Cancer Cells was Promoted by 4-Tert-octylphenol and 4-Nonylphenol via Downregulation of TGF-beta Receptor 2 and Upregulation of c-myc. Toxicological research 2011, 27(4): 253-259.
[10]
Kim YS, Hwang KA, Hyun SH, Nam KH, Lee CK, Choi KC: Bisphenol A and nonylphenol have the potential to stimulate the migration of ovarian cancer cells by inducing epithelial-mesenchymal transition via an estrogen receptor dependent pathway. Chemical research in toxicology 2015, 28(4): 662-671.
[11]
Pessina MA, Hoyt RF, Jr., Goldstein I, Traish AM: Differential regulation of the expression of estrogen, progesterone, and androgen receptors by sex steroid hormones in the vagina: immunohistochemical studies. J Sex Med 2006, 3(5): 804-814.
[12]
Piatkowski K, Kruk-Zagajewska A, Thielemann A, Kopczynski Z: [Correlation of sex hormones in blood serum and estrogen-progesterone receptors concentration in patients with laryngeal cancer]. Otolaryngol Pol 2002, 56(6): 675-681.
[13]
Vivacqua A, Recchia AG, Fasanella G, Gabriele S, Carpino A, Rago V, Di Gioia ML, Leggio A, Bonofiglio D, Liguori A et al: The food contaminants bisphenol A and 4-nonylphenol act as agonists for estrogen receptor alpha in MCF7 breast cancer cells. Endocrine 2003, 22(3): 275-284.
[14]
Shen H, Xu P, Yu W, Liu Y, Wu D: [Effects of p-nonylphenol on expression of ER protein and ER alpha mRNA of MCF-7 cells]. Sichuan da xue xue bao Yi xue ban = Journal of Sichuan University Medical science edition 2003, 34(4): 641-644.
[15]
Ge LC, Chen ZJ, Liu HY, Zhang KS, Liu H, Huang HB, Zhang G, Wong CK, Giesy JP, Du J et al: Involvement of activating ERK1/2 through G protein coupled receptor 30 and estrogen receptor alpha/beta in low doses of bisphenol A promoting growth of Sertoli TM4 cells. Toxicol Lett 2014, 226(1): 81-89.
[16]
Dong S, Terasaka S, Kiyama R: Bisphenol A induces a rapid activation of Erk1/2 through GPR30 in human breast cancer cells. Environ Pollut 2011, 159(1): 212-218.
[17]
Dance M, Montagner A, Salles JP, Yart A, Raynal P: The molecular functions of Shp2 in the Ras/Mitogen-activated protein kinase (ERK1/2) pathway. Cell Signal 2008, 20(3): 453-459.
[18]
Burns KA, Li Y, Arao Y, Petrovich RM, Korach KS: Selective mutations in estrogen receptor alpha D-domain alters nuclear translocation and non-estrogen response element gene regulatory mechanisms. The Journal of biological chemistry 2011, 286(14): 12640-12649.
[19]
Park MA, Choi KC: Effects of 4-nonylphenol and bisphenol A on stimulation of cell growth via disruption of the transforming growth factor-beta signaling pathway in ovarian cancer models. Chemical research in toxicology 2014, 27(1): 119-128.
[20]
Prins GS, Tang WY, Belmonte J, Ho SM: Developmental exposure to bisphenol A increases prostate cancer susceptibility in adult rats: epigenetic mode of action is implicated. Fertil Steril 2008, 89(2 Suppl): e41.
[21]
Yi BR, Jeung EB, Choi KC: 204 altered gene expression following exposure to bisphenol a in human ovarian cancer cells expressing estrogen receptors by microarray. Reprod Fertil Dev 2011, 23(1): 201.
[22]
Schafer TE, Lapp CA, Hanes CM, Lewis JB, Wataha JC, Schuster GS: Estrogenicity of bisphenol A and bisphenol A dimethacrylate in vitro. J Biomed Mater Res 1999, 45(3): 192-197.
[23]
Murray TJ, Maffini MV, Ucci AA, Sonnenschein C, Soto AM: Induction of mammary gland ductal hyperplasias and carcinoma in situ following fetal bisphenol A exposure. Reprod Toxicol 2007, 23(3): 383-390.
[24]
Richter CA, Birnbaum LS, Farabollini F, Newbold RR, Rubin BS, Talsness CE, Vandenbergh JG, Walser-Kuntz DR, vom Saal FS: In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol 2007, 24(2): 199-224.
[25]
Hess-Wilson JK, Webb SL, Daly HK, Leung YK, Boldison J, Comstock CE, Sartor MA, Ho SM, Knudsen KE: Unique bisphenol A transcriptome in prostate cancer: novel effects on ERbeta expression that correspond to androgen receptor mutation status. Environ Health Perspect 2007, 115(11): 1646-1653.
[26]
Boonyaratanakornkit V, Edwards DP: Receptor mechanisms mediating non-genomic actions of sex steroids. Semin Reprod Med 2007, 25(3): 139-153.
[27]
Zhang J, Zhang F, Niu R: Functions of Shp2 in cancer. J Cell Mol Med 2015, 19(9): 2075-2083.
[28]
Serrano M: SHP2: a new target for pro-senescence cancer therapies. EMBO J 2015, 34(11): 1439-1441.
[29]
Li J, Kang Y, Wei L, Liu W, Tian Y, Chen B, Lin X, Li Y, Feng GS, Lu Z: Tyrosine phosphatase Shp2 mediates the estrogen biological action in breast cancer via interaction with the estrogen extranuclear receptor. PLoS One 2014, 9(7): e102847.
[30]
He Z, Zhang SS, Meng Q, Li S, Zhu HH, Raquil MA, Alderson N, Zhang H, Wu J, Rui L et al: Shp2 controls female body weight and energy balance by integrating leptin and estrogen signals. Mol Cell Biol 2012, 32(10): 1867-1878.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186