Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel)
American Journal of Bioscience and Bioengineering
Volume 3, Issue 6, December 2015, Pages: 208-213
Received: Nov. 10, 2015; Accepted: Dec. 29, 2015; Published: Jan. 27, 2016
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Adedayo Adebiyi, Chemistry Advanced Laboratory, Sheda Science & Technology Complex (SHESTCO), P. M. B., Garki – Abuja, Nigeria
Rachael Ayo, Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria
Isaac Bello, Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria
James Habila, Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria
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The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.
Phytochemical, Guiera senegalensis, Anti-Tb, Root Extract, Mycobacterium Tuberculosis
To cite this article
Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila, Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) , American Journal of Bioscience and Bioengineering. Vol. 3, No. 6, 2015, pp. 208-213. doi: 10.11648/
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