Glutamic Acid Production from Rice Husk Using Corynebacterium glutamicum Isolated from Soil
American Journal of Bioscience and Bioengineering
Volume 4, Issue 6, December 2016, Pages: 70-76
Received: Sep. 26, 2016; Accepted: Nov. 19, 2016; Published: Jan. 17, 2017
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Authors
Musa Bishir, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
Ado Saleh Alhaji, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
Abdullahi Isa Obansa, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
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Abstract
Many different biomass of agricultural origin holds remarkable potential for conversion into valuable products thereby presenting a double sharp edge importance of sustainable resource supply and environmental protection. Glutamic acid was produced from rice husk using a novel strain of Corynebacterium glutamicum and effects of parameters optimization such as substrate concentration, temperature, pH and inoculum size were determined during the fermentation process. The wild-type (Novel) strain was inoculated into 13 g/L of the pre-treated rice husk previously added to basal medium (pH 7.2), after which fermentation began. Fermentation broth from each flask was taken aseptically after 96 h and was assayed qualitatively and quantitatively. The acid-treated and alkali-treated rice husk gave the best glutamic acid yield of 10.40g/L and 9.08g/L respectively with the wild-type strain under predetermined optimum fermentation conditions. Out of the four parameters optimized, only substrate concentration was not found to be significant on the performance of the wild-type strain in glutamate production (p > 0.05). Acid-treated rice husk hydrolysate was found to be a better substrate for L-glutamate production by the wild-type strain of C. glutamicum under the optimum fermentation conditions determined.
Keywords
Rice Husk, Wild-Type, C. glutamicum, Optimization, Glutamate
To cite this article
Musa Bishir, Ado Saleh Alhaji, Abdullahi Isa Obansa, Glutamic Acid Production from Rice Husk Using Corynebacterium glutamicum Isolated from Soil, American Journal of Bioscience and Bioengineering. Vol. 4, No. 6, 2016, pp. 70-76. doi: 10.11648/j.bio.20160406.13
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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