Glutamic Acid Production from Rice Husk Using Corynebacterium glutamicum Isolated from Soil
American Journal of Bioscience and Bioengineering
Volume 4, Issue 6, December 2016, Pages: 70-76
Received: Sep. 26, 2016; Accepted: Nov. 19, 2016; Published: Jan. 17, 2017
Views 3708      Downloads 194
Musa Bishir, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
Ado Saleh Alhaji, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
Abdullahi Isa Obansa, Department of Microbiology, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria
Article Tools
Follow on us
Many different biomass of agricultural origin holds remarkable potential for conversion into valuable products thereby presenting a double sharp edge importance of sustainable resource supply and environmental protection. Glutamic acid was produced from rice husk using a novel strain of Corynebacterium glutamicum and effects of parameters optimization such as substrate concentration, temperature, pH and inoculum size were determined during the fermentation process. The wild-type (Novel) strain was inoculated into 13 g/L of the pre-treated rice husk previously added to basal medium (pH 7.2), after which fermentation began. Fermentation broth from each flask was taken aseptically after 96 h and was assayed qualitatively and quantitatively. The acid-treated and alkali-treated rice husk gave the best glutamic acid yield of 10.40g/L and 9.08g/L respectively with the wild-type strain under predetermined optimum fermentation conditions. Out of the four parameters optimized, only substrate concentration was not found to be significant on the performance of the wild-type strain in glutamate production (p > 0.05). Acid-treated rice husk hydrolysate was found to be a better substrate for L-glutamate production by the wild-type strain of C. glutamicum under the optimum fermentation conditions determined.
Rice Husk, Wild-Type, C. glutamicum, Optimization, Glutamate
To cite this article
Musa Bishir, Ado Saleh Alhaji, Abdullahi Isa Obansa, Glutamic Acid Production from Rice Husk Using Corynebacterium glutamicum Isolated from Soil, American Journal of Bioscience and Bioengineering. Vol. 4, No. 6, 2016, pp. 70-76. doi: 10.11648/
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ahmed, Y. M., Khan, J. A., Abulnaja, K. A. andAl-Maliki, A. L. (2013). Production of glutamic acid by Corynebacteriumglutamicum using dates syrup as carbon source. African Journal of Microbiology Research, 7(19): 2072.
Amin, G. A. and Al-Talhi, A. (2007). Production of L-glutamic Acid by Immobilized Cell Reactor of the Bacterium CorynebacteriumglutamicumEntrapped into Carrageenan Gel Beads. World Applied Science, Journal, 2: 62-67.
Bergey’s Manual of Determinative Bacteriology. (2004). Eds., John G. Holt et al., 9thedn. The Williams and Wilkins, Baltimore, p.565.
Blombach, B. and Seibold, G. M. (2010) Carbohydrate metabolism in Corynebacteriumglutamicum and applications for the metabolicengineering of L-lysine production strains. Applied Microbiology and Biotechnology, 86(5): 1313-1322.
Chen, X., Chen, S., Sun, M. and Yu, Z. (2008). High yield of glutamic acid production from Bacillus substilis by solid state fermentationusing swine manure as the basis as solid substrate. Bioresource Technology, 96: 1875-1879.
Hadia, G., Shah, A. and Younis, N. (2012). Fermentative Production of Glutamate by Newly Isolated Soil Bacteria. International Journal of Pharmaceutical & Biological Archives, 3(6): 1368-1376.
Hassan, B., Asghar, M., Nadeem, S., Zubair, H., Muzammil, H. M. and Shahid, M. (2003). Isolation and Screening of Amino acids-Producing Bacteria from Milk. Biotechnology, 2(1): 18-29.
Jyothi, A. N., Sasikiran, K., Nambisan, B. and Balagopalan, C. (2005). Optimization of glutamic acid production from cassava starch factory residues using Brevibacteriumdivaricatum. Processes in Biochemistry, 40(11): 3576-3579.
Levesque, R. (2007). SPSS Programming and Data Mangement. AGiude for SPSS and SAS Users, fourth edition, SPSS Inc., Chicago III.ISBN 1- 56827-390-8.
Nakamura, J., Hirano, S. and Ito, H. (2006). L-Glutamic Acid Producing Microorganism and a Method for Producing L-Glutamic Acid. U.S. patent US20060141588A1.
Nottebrock, D., Meyer, U., Krämer, R. and Morbach, S.(2003). Molecular and biochemical characterization of mechanosensitive channels in Corynebacteriumglutamicum. FEMS Microbiology Letters, 218: 305-309.
Rakesh, K. T. and Devendra, P. S. (2013). Acid and Alkaline Pre-treatment of Lignocellulosic Biomass to Produce Ethanol as Biofuel. International Journal of Chemical Technology Research, U.S.A., 5(2): 729.
Sthiannopkao, S., Danner, H. and Braun, R. (2001). Use of Grass Sap as an Ingredient in Glutamate Production. Thammasat International Journal of Science and Technology, 6: 3-4.
Vijayalakshm, P. and Sarvamangala D. (2011). Production of L-glutamic acid by Arthrobacterglobiformis MTCC 4299 fruits of Mimusops Elengilinn. International Journal of Applied Biology and Pharmaceutical Technology, 2: 167-173.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186