Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp.
American Journal of Bioscience and Bioengineering
Volume 4, Issue 6, December 2016, Pages: 90-95
Received: Aug. 29, 2016; Accepted: Dec. 30, 2016; Published: Jan. 24, 2017
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P. C. Madhu, Department of Biotechnology, MET’S School of Engineering, Kuruvilassery, Mala, Kerala, India
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Food grade proteases are proteyolytic enzymes having application in baking, food processing, protein modification etc. As a commodity product, pressure on protease market is on prize reduction and increasing performance. Hence our objective was to isolate a potent protease-producing microorganism and formulate a cost effective medium for neutral protease synthesis by the potent microbial culture. In order to achieve the objective, a proteolytic bacterium was isolated from soil using milk agar medium and the bacteria was identified as Bacillus sp. by morphological and biochemical characterization. Dairy industry effluent was then studied as a medium for neutral protease synthesis by the potent bacteria. Supplementation of mineral salt to the medium did not show profound influence of environmental factors such as medium pH, incubation temperature, agitation rate and incubation time on enzyme production. Optimum enzyme titers were found at pH7 when incubated at 37°C and 120 rpm 48 h. Dairy industry effluent was thus found to be a cost effective medium for neutral protease synthesis by Bacillus sp.
Dairy Industry, Dairy Effluent, Bacillus sp., Neutral Protease, Milk Agar, Mineral Salt Medium
To cite this article
P. C. Madhu, Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp., American Journal of Bioscience and Bioengineering. Vol. 4, No. 6, 2016, pp. 90-95. doi: 10.11648/j.bio.20160406.15
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