Comparison of the Antiparasitic Activity of Bauhinia rufescens Leaves Extracts and Metronidazole Against Gairdia lamblia
American Journal of Bioscience and Bioengineering
Volume 5, Issue 5, October 2017, Pages: 104-108
Received: Oct. 4, 2017; Accepted: Oct. 26, 2017; Published: Nov. 22, 2017
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Authors
Mohamed Ismail Garbi, Department of Microbiology and Parasitology, Faculty of Medical Laboratory Sciences, Ibn Sina University, Khartoum, Sudan
Elbadri Elamin Osman, Department of Microbiology, Faculty of Pure and Applied Sciences, International University of Africa, Khartoum, Sudan
Ahmed Saeed Kabbashi, Department of Microbiology, Faculty of Medical Laboratory Sciences, International University of Africa, Khartoum, Sudan
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Abstract
Giardia is a flagellate protozoan with worldwide distribution that causes significant gastrointestinal diseases in a wide variety of vertebrates including cats and human. Gairdia lamblia is one of the intestinal protozoa that cause public health problems in most developing countries as well as some developed countries. This study was carried out to evaluate Antigiardial activity of Bauhinia rufsescens (Leaves) petroleum ether and methanolic extracts in vitro test were perform using four concentrations: (1000, 500, 250 and 125 ppm). The highest activity against G. lameblia with respect to time was obtained from petroleum ether extract which exhibited 77.12% mortality within 72 h in 1000 ppm concentration, followed by the same extract which exhibited 76.04% mortality within 72 h with concentration of 500 ppm. On the other hand, the lowest antigiardial activity was recorded by B. rufescens petroleum ether extract 82.8% mortality with 125 ppm concentration within 24 hours, with whereas Metronidazol, a pure compound (positive control) showed 83.42% mortality within 72 hours. This result shows that the most potent antigiardial activity was demonstrated in B. rufescens petroleum ether extract at all concentrations (i.e. 74% mortality within 72 hours) and that B. rufescens species, as claimed also by tradition, is a promising species in treating G. lamblia.
Keywords
Bauhinia rufescens, Gairdia lameblia, Metronidazol
To cite this article
Mohamed Ismail Garbi, Elbadri Elamin Osman, Ahmed Saeed Kabbashi, Comparison of the Antiparasitic Activity of Bauhinia rufescens Leaves Extracts and Metronidazole Against Gairdia lamblia, American Journal of Bioscience and Bioengineering. Vol. 5, No. 5, 2017, pp. 104-108. doi: 10.11648/j.bio.20170505.12
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Iran, M, Kezaeian, Mal Izaddoost. M. (2006) In vitro antitrichomonas activity of Allium ehrtifolium (Persian shallot) in comparison with metrondizole. Iranian Journal of Rubi Health 35: 92 -94.
[2]
Pratibha Tiwari, Divya Singh and Man Mohan Singh. (2008) Anti-Trichomonas activity of Sapindus saponins, a candidate for development as microbicidal contraceptive, Journal of Antimicrobial Chemotherapy 62(3):526-534.
[3]
Flavia M. M. Amaral, Maria Nilce S. Ribeiro, José M. Barbosa-Filho, Aramys S. Reis, Flávia R. F. Nascimento, Rui O. Macedo (2006). Plants and chemical constituents with giardicidal activity. Brazilian Journal of Pharmacognosy 16: 696-720.
[4]
Koko, W. S. Mesaik M. A, Yousaf, S. Galal, M. Iqbal M. Choudhary. (2008) In vitro immunomodulating properties of selected Sudanese medicinal plants. Journal of Ethnopharmacology, 118, 26-34.
[5]
Burkill, H. M. (1995). The Useful Plants of West Tropical Africa. Vol. II Royal Botanic Gardens, Kew, London, UK. pp 61-67.
[6]
Ayensu, E. S. (1978). The Medicinal and Poisonous Plants of Southern and Eastern Africa, Reference Publications Inc., Algonac Michigan.
[7]
Newman, R. D. Moore, S. R. Lima, A. A. Nataro, J. P. Guerrant, R. L. Sears, C. L. (2001). A longitudinal study of Giardia lamblia infection in north-east Brazilian children. Trop. Med. Int. Health 6:624–634.
[8]
Legator, M. S. Connor, T. H. Stoeckel, M. (1975). Detection of mutagenic activity of metronidazole and nitridazole in body fluids of humans and mice. Science 188:1118–1119.
[9]
Rustia, M. Shubik, P. (1972). Induction of lung tumors and malignant lymphomas in mice by metronidazole. J. Natl. Cancer. Inst. 48:721–729.
[10]
Llibre, J. M. Tor, J. Manterola, J. M. Carbonell, C. Foz, M. (1989). Blastocystis hominis chronic diarrhoea in AIDS patients. Lancet 1:221.
[11]
Campanati L, Monteiro-Leal LH (2002). The effects of the antiprotozoal drugs metronidazole and furazolidone on trophozoites of Giardia lamblia (P1 strain). Parasitol Res 88: 80-85.
[12]
Harris, J. C. Plummer, S. Turner, M. P. Lloyd, D. (2000). The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology 146: 3119-3127.
[13]
Harris, J. C. Plummer, S. Lloyd, D. (2001). Antigiardial drugs. Appl Microbiol Biotechnol 57: 614-619.
[14]
Morgan, U. M. Reynoldson, J. A. Thompson, R. C. A. (1993). Activities of several benzimidazoles and tubulin inhibitors against Giardia spp. in vitro. Antimicrobial Agents Chemistry, 37: 328-331.
[15]
Petri, Jr. W. A. (2003). Therapy of intestinal protozoa. Trends Parasitology, 19: 523-526.
[16]
Upcrof, J. A. Dunn, L. A. Wrigh, J. M. Benakli, K. Upcrof, P. Vanelle, P. (2006). 5- nitroimidazole drugs effective against metronidazole- resistant. Trichomonas vaginalis and Giardia dodenalis spp. Animicrobial agents and Chemotherapy 50, 344-347.
[17]
Harborne, J. B. (1984). Phytochemical methods. Second edition. Chapman and Hall.
[18]
Cedillo-Rivera, R., Chave, B., Gonzalez-Robles, A., Tapia-Contreras, A., Yepez-Mulia, L., (2002). In vitro effect of nitazoxanide against Entamoba histolytica, Gairdia lamblia and Trichomonas vaginalis trophozoites. The journal of eukaryotic microbiology 49, 201-208.
[19]
Arguello-Garcia, R. Cruz-suto, M. Romero-Montoya, L. Ortega-Pierres, G. (2004) Variability and variation in drug susceptibility among Giardia duodenalis isolates and clones exposed to 5-nitromidazoles and benzimidazoles in vitro. Journal of Antimicrobial chemotharpy 54, 711-721.
[20]
Boreham, P. F. L. Phillips, R. E. and Shepherd, R. W. (1984). The sensitivity of Giardia intestinalis to drugs in vitro. Journal of Antimicrobial Chemotherapy, 14:449-461.
[21]
Maydell, H., and Von, J., (1986). Trees and shrubs of the Sahel, their characteristics and uses. Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) GmbH, Dag-Hammarskj ld-Weg1+2, D6236 Eschborn 1, Federal Rebublic of Germany.
[22]
Calzada F, Meckes M, Cedillo-Rivera R, Tapia-Contreras A, Mata R (1998). Screening of Mexican medicinal plants for antiprotozoal activity. Pharm Biol, 36:305-309.
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