Enhance Maize <i>(Zea mays L.)</i> Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour

doi:doi:10.11648/j.bio.20190702.11

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Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour

Published in American Journal of Bioscience and Bioengineering (Volume 7, Issue 2)

Received: 4 March 2019 Accepted: 19 March 2019 Published: 7 May 2019

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Abstract

The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.

Published in	American Journal of Bioscience and Bioengineering (Volume 7, Issue 2)
DOI	10.11648/j.bio.20190702.11
Page(s)	28-33
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Maize, Biotic Stress, Anthranilate Synthase, Gus Assay, Particle Bombardment

References

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APA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. (2019). Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. American Journal of Bioscience and Bioengineering, 7(2), 28-33. https://doi.org/10.11648/j.bio.20190702.11

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ACS Style

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am. J. BioSci. Bioeng. 2019, 7(2), 28-33. doi: 10.11648/j.bio.20190702.11

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AMA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am J BioSci Bioeng. 2019;7(2):28-33. doi: 10.11648/j.bio.20190702.11

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@article{10.11648/j.bio.20190702.11,
  author = {Karim Mohamed Beltagy and Manal Moustafa Abdel-Rahman and Hanaa Mahdy Abouzied and Samia Ahmed Madkour},
  title = {Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {28-33},
  doi = {10.11648/j.bio.20190702.11},
  url = {https://doi.org/10.11648/j.bio.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190702.11},
  abstract = {The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.},
 year = {2019}
}

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TY  - JOUR
T1  - Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment
AU  - Karim Mohamed Beltagy
AU  - Manal Moustafa Abdel-Rahman
AU  - Hanaa Mahdy Abouzied
AU  - Samia Ahmed Madkour
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190702.11
DO  - 10.11648/j.bio.20190702.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190702.11
AB  - The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.
VL  - 7
IS  - 2
ER  -

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Author Information

Karim Mohamed Beltagy

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Manal Moustafa Abdel-Rahman

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Hanaa Mahdy Abouzied

Agronomy Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Samia Ahmed Madkour

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt

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Plain Text BibTeX RIS

APA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. (2019). Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. American Journal of Bioscience and Bioengineering, 7(2), 28-33. https://doi.org/10.11648/j.bio.20190702.11

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ACS Style

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am. J. BioSci. Bioeng. 2019, 7(2), 28-33. doi: 10.11648/j.bio.20190702.11

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AMA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am J BioSci Bioeng. 2019;7(2):28-33. doi: 10.11648/j.bio.20190702.11

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@article{10.11648/j.bio.20190702.11,
  author = {Karim Mohamed Beltagy and Manal Moustafa Abdel-Rahman and Hanaa Mahdy Abouzied and Samia Ahmed Madkour},
  title = {Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {28-33},
  doi = {10.11648/j.bio.20190702.11},
  url = {https://doi.org/10.11648/j.bio.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190702.11},
  abstract = {The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment
AU  - Karim Mohamed Beltagy
AU  - Manal Moustafa Abdel-Rahman
AU  - Hanaa Mahdy Abouzied
AU  - Samia Ahmed Madkour
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190702.11
DO  - 10.11648/j.bio.20190702.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190702.11
AB  - The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.
VL  - 7
IS  - 2
ER  -

Copy | Download