American Journal of Bioscience and Bioengineering

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Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring

Received: 14 February 2015    Accepted: 14 February 2015    Published: 12 March 2015
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Abstract

Unfortunately, the pesticides pollute a large palette from environment: plants, organisms, soil and water. Using monitoring tools, like chemical biosensors, the use of pesticides can be put under control. Microelectronics offers the convenient transducers. Borrowing micro-technological processes, the enzymatic biosensors can be easily integrated onto the Silicon wafers. This paper proposes a biosensor for paraoxon pesticide detection, as a small piece from a decontamination soil technology plan. The paper reveals the pesticide detection principle, by paraoxon hydrolysis assisted by the Acetyl-cholinesterase enzyme, as key receptor. The enzyme is entrapped on a porous thin layer by adsorption. An advantageous method of the porous intermediate material anchored onto the Si-substrate, converting p-type Si in Si-porous by anodization, is described. Then, some technological steps, with tests and microscopy analysis are presented. Finally, the preliminary tests of the developed biosensor with the AcHE enzyme immobilized onto the Si-porous layer, are discussed.

DOI 10.11648/j.bio.s.2015030301.11
Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 3-1, June 2015)

This article belongs to the Special Issue Bio-Electronics: Biosensors, Biomedical Signal Processing, and Organic Engineering

Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biosensor, Design, Enzyme, Si-porous, Electrodes

References
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Author Information
  • Dept. of Electronics, Polytechnic University of Bucharest, BioNEC Group, Bucharest, Romania

  • Institute of Microtechnology, Bucharest, Romania

  • Institute of Microtechnology, Bucharest, Romania

  • Institute of Microtechnology, Bucharest, Romania

  • Institute of Microtechnology, Bucharest, Romania

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  • APA Style

    Cristian Ravariu, Elena Manea, Alina Popescu, Cecilia Podaru, Catalin Parvulescu. (2015). Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring. American Journal of Bioscience and Bioengineering, 3(3-1), 1-6. https://doi.org/10.11648/j.bio.s.2015030301.11

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    ACS Style

    Cristian Ravariu; Elena Manea; Alina Popescu; Cecilia Podaru; Catalin Parvulescu. Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring. Am. J. BioSci. Bioeng. 2015, 3(3-1), 1-6. doi: 10.11648/j.bio.s.2015030301.11

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    AMA Style

    Cristian Ravariu, Elena Manea, Alina Popescu, Cecilia Podaru, Catalin Parvulescu. Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring. Am J BioSci Bioeng. 2015;3(3-1):1-6. doi: 10.11648/j.bio.s.2015030301.11

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  • @article{10.11648/j.bio.s.2015030301.11,
      author = {Cristian Ravariu and Elena Manea and Alina Popescu and Cecilia Podaru and Catalin Parvulescu},
      title = {Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {3},
      number = {3-1},
      pages = {1-6},
      doi = {10.11648/j.bio.s.2015030301.11},
      url = {https://doi.org/10.11648/j.bio.s.2015030301.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bio.s.2015030301.11},
      abstract = {Unfortunately, the pesticides pollute a large palette from environment: plants, organisms, soil and water. Using monitoring tools, like chemical biosensors, the use of pesticides can be put under control. Microelectronics offers the convenient transducers. Borrowing micro-technological processes, the enzymatic biosensors can be easily integrated onto the Silicon wafers. This paper proposes a biosensor for paraoxon pesticide detection, as a small piece from a decontamination soil technology plan. The paper reveals the pesticide detection principle, by paraoxon hydrolysis assisted by the Acetyl-cholinesterase enzyme, as key receptor. The enzyme is entrapped on a porous thin layer by adsorption. An advantageous method of the porous intermediate material anchored onto the Si-substrate, converting p-type Si in Si-porous by anodization, is described. Then, some technological steps, with tests and microscopy analysis are presented. Finally, the preliminary tests of the developed biosensor with the AcHE enzyme immobilized onto the Si-porous layer, are discussed.},
     year = {2015}
    }
    

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    T1  - Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring
    AU  - Cristian Ravariu
    AU  - Elena Manea
    AU  - Alina Popescu
    AU  - Cecilia Podaru
    AU  - Catalin Parvulescu
    Y1  - 2015/03/12
    PY  - 2015
    N1  - https://doi.org/10.11648/j.bio.s.2015030301.11
    DO  - 10.11648/j.bio.s.2015030301.11
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.s.2015030301.11
    AB  - Unfortunately, the pesticides pollute a large palette from environment: plants, organisms, soil and water. Using monitoring tools, like chemical biosensors, the use of pesticides can be put under control. Microelectronics offers the convenient transducers. Borrowing micro-technological processes, the enzymatic biosensors can be easily integrated onto the Silicon wafers. This paper proposes a biosensor for paraoxon pesticide detection, as a small piece from a decontamination soil technology plan. The paper reveals the pesticide detection principle, by paraoxon hydrolysis assisted by the Acetyl-cholinesterase enzyme, as key receptor. The enzyme is entrapped on a porous thin layer by adsorption. An advantageous method of the porous intermediate material anchored onto the Si-substrate, converting p-type Si in Si-porous by anodization, is described. Then, some technological steps, with tests and microscopy analysis are presented. Finally, the preliminary tests of the developed biosensor with the AcHE enzyme immobilized onto the Si-porous layer, are discussed.
    VL  - 3
    IS  - 3-1
    ER  - 

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