American Journal of Bioscience and Bioengineering

| Peer-Reviewed |

The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair

Received: 29 May 2015    Accepted: 01 June 2015    Published: 30 June 2015
Views:       Downloads:

Share This Article

Abstract

Background: About 50% of peripheral nerve injuries leave permenant disability. Trials to enhance nerve repair using stem cells are undergoing. Adipose derived mesenchymal stem cells (ADSCs) are good candidates for cellular-induced regeneration. Aim of the Work: The aim of the present work is to evaluate the effect of ADSCs on enhancing repair of peripheral nerve injuries. Subjects and Methods: This study was conducted on 18 patients with recent trauma involving nerve injuries at the wrist region. Patients were divided into: Group I: underwent surgical end-to-end closure GroupII: underwent surgical end-to-end closure and injection of ADSCs into the nerve sheath and surrounding tissue. Both groups were followed up using clinical assessment using Sensory Recovery Grading and Muscle Strength Grading systems in addition to electrophysiological nerve conduction tests. Results and Conclusions: This study collected a range of 1 to 2 x106 ADSCs from an average of 100-150 ml fat. After 1 year, stem cell group showed significantly higher motor and sensory improvement in ulnar nerve injury; while in the median nerve injury, stem cell group showed higher motor recovery with no difference in sensory recovery.

DOI 10.11648/j.bio.s.2015030401.13
Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 4-1, July 2015)

This article belongs to the Special Issue Stem Cells for Neuro-Regeneration: Where Do We Stand

Page(s) 18-23
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Peripheral Nerve, Adipose Stem Cells, Regeneration

References
[1] Georgiou M, Golding JP,Loughlin AJ, Kingham PJ, Phillips J B (2015): Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve. Biomaterials 37:242-251.
[2] Menorca RM, Fussell TS, Elfar JC. Nerve physiology: mechanisms of injury and recovery. Hand Clin.2013;29:317–330.
[3] Rodrigues MC, Rodrigues Jr. AA, Glover LE, Voltarelli J, Borlongan CV (2012): Peripheral nerve repair with cultured Schwann cells: getting closer to the clinics. Sc W J :413091.
[4] Perrin FE, Lacroix M, Aviles-Trieueros M and David S (2005): Involvement of monocyte chemoattractant protein-1 macrophage inflammatory protein1a and interleukin 1B in Wallarian degeneration. Brain 128(4):854-866.
[5] Coleman MP and Freeman MR (2010): Wallarian degeneration, Wlds and Nmnat. An Rev Neurosc 33:245-267.
[6] Jiang L, Zhu JK, Liu L, Xiang P, Hu J, Yu WH (2008): Differentiation of rat adipose tissue-derived stem cells into Schwann-like cells in vitro. Neuroreport 19:1015-1019/
[7] Kaewkhaw R, Scutt AM, Haycock JW (2011): Anatomical site influences the differentiation of adipose-derived stem cells for Schwann-cell phenotype and function. Glia 59:734-749.
[8] Bertassoli BM, de Assis Neto AC, Oliveira FD, Arroyao M, Ferrao J, da Siva J, Pignatari G, Braga P (2013): Mesenchymal stem cells -emphasis in adipose tissue. Braz Arch. Biol Technol 56(4).
[9] Jiang L, Zhu JK, Liu XL, Xiang P, Hu J, Yu WH (2008): Differentiation of rat adipose tissue-derived stem cells into Schwann-like cells in vitro. Neuroreport 19:1015-1019.
[10] Kingham PJ, Kalbermaten DF, Mahay D, Armstrong SJ, Wiberg M, Terenghi G (2007): Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro. Exp Neurol 207:267-274.
[11] Radtke C, Schmitz B, Spies M, Kocsis JD, Vogt PM (2009): Peripheral glial cell differentiation from neurospheres derived from adipose mesenchyal stem cells. Int J Dev Neurosci 27:817-823.
[12] Wudgerow AD, Salibian AA, Lalezari S, Evans GR (2013): Neuromodulatory nerve regeneration: adipose tissue-derived stem cells and neurotrophic mediation in peripheral nerve regeneration. J Neurosci Res 91:1517-1524.
[13] Kolar MK, Kingham PJ (2014): Regenerative effects of adipose-tissue-derived stem cells for treatment of peripheral nerve injuries. Biochem Soc Trans 42:697-701.
[14] Marconi S, Castiglione G, Turano E, Bissolotti G, Angiari S, Farinazzo (2012): Tissue Eng Part A 18:1264-1272.
[15] Reid AJ, Sun M, Wiberg M, Downes S, Terenghi G, Kingham PJ (2011): Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons from apoptosis. Neuroscience 199:515-522.
[16] Kingham PJ, Kolar MK, Novikova LN, Novikov LN, Wiberg M (2014): Stimulating the neurotrophic and angiogenic properties of human adipose-derived stem cells enhances nerve repair. Stem Cells Dev, 23: 741–754.
[17] Zack-Wiliams S, Butler P, and Kalaskar D (2015): Current progress in use of adipose derived stem cells in peripheral nerve regeneration. W J Stem Cells 7(1):51-64.
[18] Mackinnon SE and Dellon AL (1988): Results of nerve repair and grafting. Surgery of the peripheral nerve Thieme 8 (4):110-115.
[19] Fairbairn NG, Meppelink AM, Ng-Glazier J, Randolph MA, Winograd JM (2015): Augmenting peripheral nerve regeneration using stem cells: A review of current opinion. W J Stem Cells 7(1):11-26.
[20] Whally K ( 2014): Schwann cells provide life support for axons. Nature Rev Neuroscience 15:689-699
[21] Faroni A, Rothwell SW, Grolla AA, Terenghi G, Magnaghi V and Verkhratsky A (2013): Differentiation of adipose derived stem cells into Schwann cell phenotype induced expression of P2X receptors that control cell death. Cell Death and Disease 4:e743.
[22] Mantovani C, Terenghi G, Shawcross SG (2012): Isolation of adult stem cells and their differentiation to Schwann cells. Methods Mol Biol. 916:47-57.
[23] Joyce N, Annett G, Wirthlin L, Olson S, Bauer G and Nolta J (2010): Mesenchymal stem cells for the treatment of neurodegenerative disease. Regen Med 5(6):933-946.
[24] Hass R, Kasper C, Bohm S and Jacobs R (2011): Different populations and sources of sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. Communication and Signaling 9:12.
[25] Strem BM, Hicok KC, Zhu M. (2005): Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 54:132–41.
[26] Gimble JM, Katz AJ, Bunnell BA.(2007): Adipose-derived stem cells for regenerative medicine. Circ Res;100:1249–60.
[27] Liu G, Cheng Y, Feng Y, Pang C, Wang Y, Jia H and Tong X (2011): Adipose derived stem cells promote peripheral nerve repair. Arch Med Sci 7(4):592-596.
[28] Dong MM and Yi TH (2010): Stem cell and peripheral nerve injury and repair. Facial Plastic Surgery 26(5):4d21-427.
[29] Akita S, Akino K, Hirano A, Ohtsuru A and Yamashita S ( 2010): Mesenchymal stem cell therapy for cutaneous radiation syndrome. Health Phys 98(6):858-862.
[30] Boquest AC, Shahdadfar A, Brinchmann JE and Collas P (2006): Isolation of stromal stem cells from human adipose tissue. Methods Mol biol 325:35-46.
[31] Kingham PJ, Kalbermatten DF, Mahay D Armstrong SJ, Wiberg M, Terenghi (2007): Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro. Exp Neurol 207:267-274.
[32] Keilhoff G, Stang F, Goihl A, Wolf G, Fansa H (2006): Transdifferentiated mesenchymal stem cells as alternative therapy in supporting nere regeneration and myelination. Cell Mol Neurobiol 26:1235-1252.
Author Information
  • Plastic Surgery Department, Faculty of Medicine, Tanta University, Tanta, Egypt

  • Clinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

  • Clinical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt

  • Plastic Surgery Department, Faculty of Medicine, Tanta University, Tanta, Egypt

  • Tissue Culture and Vaccine Department, VACSERA, Cairo, Egypt

Cite This Article
  • APA Style

    Ayad H., Zahra M., Gabr H., Ismaeil A., Abo Elkheir Y. (2015). The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair. American Journal of Bioscience and Bioengineering, 3(4-1), 18-23. https://doi.org/10.11648/j.bio.s.2015030401.13

    Copy | Download

    ACS Style

    Ayad H.; Zahra M.; Gabr H.; Ismaeil A.; Abo Elkheir Y. The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair. Am. J. BioSci. Bioeng. 2015, 3(4-1), 18-23. doi: 10.11648/j.bio.s.2015030401.13

    Copy | Download

    AMA Style

    Ayad H., Zahra M., Gabr H., Ismaeil A., Abo Elkheir Y. The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair. Am J BioSci Bioeng. 2015;3(4-1):18-23. doi: 10.11648/j.bio.s.2015030401.13

    Copy | Download

  • @article{10.11648/j.bio.s.2015030401.13,
      author = {Ayad H. and Zahra M. and Gabr H. and Ismaeil A. and Abo Elkheir Y.},
      title = {The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair},
      journal = {American Journal of Bioscience and Bioengineering},
      volume = {3},
      number = {4-1},
      pages = {18-23},
      doi = {10.11648/j.bio.s.2015030401.13},
      url = {https://doi.org/10.11648/j.bio.s.2015030401.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.bio.s.2015030401.13},
      abstract = {Background: About 50% of peripheral nerve injuries leave permenant disability. Trials to enhance nerve repair using stem cells are undergoing. Adipose derived mesenchymal stem cells (ADSCs) are good candidates for cellular-induced regeneration. Aim of the Work: The aim of the present work is to evaluate the effect of ADSCs on enhancing repair of peripheral nerve injuries. Subjects and Methods: This study was conducted on 18 patients with recent trauma involving nerve injuries at the wrist region. Patients were divided into: Group I: underwent surgical end-to-end closure GroupII: underwent surgical end-to-end closure and injection of ADSCs into the nerve sheath and surrounding tissue. Both groups were followed up using clinical assessment using Sensory Recovery Grading and Muscle Strength Grading systems in addition to electrophysiological nerve conduction tests. Results and Conclusions: This study collected a range of 1 to 2 x106 ADSCs from an average of 100-150 ml fat. After 1 year, stem cell group showed significantly higher motor and sensory improvement in ulnar nerve injury; while in the median nerve injury, stem cell group showed higher motor recovery with no difference in sensory recovery.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - The Role of Adipose Derived Mesenchymal Stem Cells in Enhancement of Peripheral Nerve Injuries Repair
    AU  - Ayad H.
    AU  - Zahra M.
    AU  - Gabr H.
    AU  - Ismaeil A.
    AU  - Abo Elkheir Y.
    Y1  - 2015/06/30
    PY  - 2015
    N1  - https://doi.org/10.11648/j.bio.s.2015030401.13
    DO  - 10.11648/j.bio.s.2015030401.13
    T2  - American Journal of Bioscience and Bioengineering
    JF  - American Journal of Bioscience and Bioengineering
    JO  - American Journal of Bioscience and Bioengineering
    SP  - 18
    EP  - 23
    PB  - Science Publishing Group
    SN  - 2328-5893
    UR  - https://doi.org/10.11648/j.bio.s.2015030401.13
    AB  - Background: About 50% of peripheral nerve injuries leave permenant disability. Trials to enhance nerve repair using stem cells are undergoing. Adipose derived mesenchymal stem cells (ADSCs) are good candidates for cellular-induced regeneration. Aim of the Work: The aim of the present work is to evaluate the effect of ADSCs on enhancing repair of peripheral nerve injuries. Subjects and Methods: This study was conducted on 18 patients with recent trauma involving nerve injuries at the wrist region. Patients were divided into: Group I: underwent surgical end-to-end closure GroupII: underwent surgical end-to-end closure and injection of ADSCs into the nerve sheath and surrounding tissue. Both groups were followed up using clinical assessment using Sensory Recovery Grading and Muscle Strength Grading systems in addition to electrophysiological nerve conduction tests. Results and Conclusions: This study collected a range of 1 to 2 x106 ADSCs from an average of 100-150 ml fat. After 1 year, stem cell group showed significantly higher motor and sensory improvement in ulnar nerve injury; while in the median nerve injury, stem cell group showed higher motor recovery with no difference in sensory recovery.
    VL  - 3
    IS  - 4-1
    ER  - 

    Copy | Download

  • Sections