Simultaneous Administration of Aqueous Extract of <i>Rosmarinus officinal</i> with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs

Azab Elsayed Azab; Mohamed Omar Albasha

doi:doi:10.11648/j.bio.20150305.20

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Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs

Azab Elsayed Azab, Mohamed Omar Albasha

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)

Received: 5 September 2015 Accepted: 10 October 2015 Published: 22 October 2015

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Abstract

Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2^nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3^rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4^th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.

Published in	American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)
DOI	10.11648/j.bio.20150305.20
Page(s)	80-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hepatoprotective, Renoprotective, Rosemary (Rosmarinus officinalis), Male Guinea Pig, Nicotine

References

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Azab Elsayed Azab, Mohamed Omar Albasha. (2015). Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. American Journal of Bioscience and Bioengineering, 3(5), 80-86. https://doi.org/10.11648/j.bio.20150305.20

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Azab Elsayed Azab; Mohamed Omar Albasha. Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. Am. J. BioSci. Bioeng. 2015, 3(5), 80-86. doi: 10.11648/j.bio.20150305.20

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Azab Elsayed Azab, Mohamed Omar Albasha. Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. Am J BioSci Bioeng. 2015;3(5):80-86. doi: 10.11648/j.bio.20150305.20

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@article{10.11648/j.bio.20150305.20,
  author = {Azab Elsayed Azab and Mohamed Omar Albasha},
  title = {Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {80-86},
  doi = {10.11648/j.bio.20150305.20},
  url = {https://doi.org/10.11648/j.bio.20150305.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.20},
  abstract = {Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.},
 year = {2015}
}

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TY  - JOUR
T1  - Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs
AU  - Azab Elsayed Azab
AU  - Mohamed Omar Albasha
Y1  - 2015/10/22
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.20
DO  - 10.11648/j.bio.20150305.20
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 80
EP  - 86
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.20
AB  - Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.
VL  - 3
IS  - 5
ER  -

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Azab Elsayed Azab

Department of Zoology, Faculty of Science, Zawia University, Alejelat, Libya
Mohamed Omar Albasha

Department of Zoology, Faculty of Science, Zawia University, Alejelat, Libya

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Azab Elsayed Azab, Mohamed Omar Albasha. (2015). Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. American Journal of Bioscience and Bioengineering, 3(5), 80-86. https://doi.org/10.11648/j.bio.20150305.20

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Azab Elsayed Azab; Mohamed Omar Albasha. Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. Am. J. BioSci. Bioeng. 2015, 3(5), 80-86. doi: 10.11648/j.bio.20150305.20

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Azab Elsayed Azab, Mohamed Omar Albasha. Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs. Am J BioSci Bioeng. 2015;3(5):80-86. doi: 10.11648/j.bio.20150305.20

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@article{10.11648/j.bio.20150305.20,
  author = {Azab Elsayed Azab and Mohamed Omar Albasha},
  title = {Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {80-86},
  doi = {10.11648/j.bio.20150305.20},
  url = {https://doi.org/10.11648/j.bio.20150305.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.20},
  abstract = {Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.},
 year = {2015}
}

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TY  - JOUR
T1  - Simultaneous Administration of Aqueous Extract of Rosmarinus officinal with Nicotine Resulted in Prevention of Induced Hepatorenal Toxicity in Guinea Pigs
AU  - Azab Elsayed Azab
AU  - Mohamed Omar Albasha
Y1  - 2015/10/22
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.20
DO  - 10.11648/j.bio.20150305.20
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 80
EP  - 86
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.20
AB  - Rosemary extracts have a high scavenging capacity of different types of reactive oxygen and nitrogen species, mostly free radicals. The present work aimed to evaluate the effectiveness of aqueous extract of rosemary as a natural source of antioxidants to minimize the harmful effects of nicotine induced hepatorenal toxicity in Guinea pigs. In this study, twenty four adult male Guinea pigs were used for this study and divided into four groups. The first group was control group, the 2nd was the rosemary group orally received rosemary (220 mg/kg body weight /day), the 3rd was the experimental and received intraperitoneal injection of nicotine (6 mg/kg body weight /day), the 4th one co-administered intraperitoneal injection of nicotine (6 mg/kg body weight /day) and rosemary (220 mg/kg body weight /day) orally by gavage for 30 days. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, total proteins, albumin, and globulin concentrations, albumin concentration/globulin concentration (A/G) ratio, urea, uric acid, creatinine, sodium ion, and potassium ion concentrations. In nicotine treated animals, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyl transferase activities, urea, uric acid, creatinine, and potassium ion concentrations were significantly (p<0.05), increased as compared to the control group. On the other hand, serum total proteins, albumin, and sodium ion concentrations of nicotine treated Guinea pigs, were significantly (p<0.05), decreased compared with control animals. But, globulin concentrations and A/G ratio were non significantly changed. Co-administration of rosemary significantly improved all biochemical parameters. It can be concluded that, simultaneous administration of aqueous extract of rosemary with nicotine resulted in prevention of induced hepatorenal toxicity in Guinea pigs. It is recommended that the heavy smokers should be advised to take rosemary as antioxidant to prevent the hepatorenal toxicity. Further studies are necessary to elucidate exact mechanism of hepatorenal protection and potential usefulness of aqueous extract of rosemary as a protective agent against nicotine induced hepatorenal toxicity in clinical trials.
VL  - 3
IS  - 5
ER  -

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