Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State

Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O.; Ogueji E. O.

doi:doi:10.11648/j.bio.20130106.11

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Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State

Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., Ogueji E. O.

Published in American Journal of Bioscience and Bioengineering (Volume 1, Issue 6)

Received: 7 September 2013 Accepted: Published: 20 October 2013

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Abstract

Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.

Published in	American Journal of Bioscience and Bioengineering (Volume 1, Issue 6)
DOI	10.11648/j.bio.20130106.11
Page(s)	75-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Antibiotics, Plasmid, S. aureus, P. aeruginosa, E. coli, Wound Infection, Abakaliki

References

[1]	Bowler PG, Duerden BI & Amstrong DG. Wound microbiology and associated approaches to wound management. Clinical Microbiology Review 2001, 14:244-269.
[2]	Akingbade OA, Balogun SA, Ojo DA, Afolabi RO, Motayo BO, Okerentugba PO & Okonko IO. Plasmid profile analysis of multidrug resistant Pseudomonas aeruginosa isolated from wound infections in South West, Nigeria. World Applied Sciences Journal 2012, 20 (6): 766-775.
[3]	Mulu A, Moges F, Tesema B & Kassu A. Patterns and multiple drug resistance of bacterial pathogens at university of Gondar teaching hospital, Northwest Ethiopia. Ethiopian Medical Journal. 2006, 44(2): 125-131.
[4]	Dionigi R, Rovera F, Dionigi G, Imperatori A, Ferrari A, Dionigi P & Dominioni L. Risk factors in surgery. Journal of Chemotherapy 2001, 13: 6-11.
[5]	Patwardhan RB, Dhakephalkar PK, Niphadkar KB & Chopade BA. A study on nosocomial pathogens in ICU with special reference to multiresistant Acinetobacter baumannii harbouring multiple plasmids. Indian Journal of Medical Research. 2008, 128:178-187.
[6]	Hosein IK, Hill DW, Jenkins LE & Magee JT. Clinical significance of emergence of bacterial resistance in the hospital environment. Sym. Ser. Soc Journal of Applied Microbiology, 2001, 31: 90S-7S.
[7]	Smith S, Ganiyu O, John R, Fowora M, Akinsinde K & Odeigah P. Antimicrobial Resistance and Molecular Typing of Pseudomonas aeruginosa Isolated from Surgical Wounds in Lagos, Nigeria. Acta Medica Iranica 2012, 50: 433-438.
[8]	Sazakli E, Leotsinidis M, Vantarakis A & Papapetropoulou M Comparative typing of Pseudomonas species isolated from the aquatic environment in Greece by SDS-PAGE and RAPD analysis. Journal of Applied Microbiology 2005, 99: 1191-1203.
[9]	Brooks GF, Brutel S & Morse SA. Jawetz, Melnick, and Adelberg’s Medical Microbiology. Twenty-third edition. McGraw Hill Companies Inc., New York, USA 2004, PP. 3-4.
[10]	Ofomata GEK. Nigeria in Maps (Eastern States). Ofomata Edition Ethiopia Publisher, Benin City 1975, PP. 45- 46.
[11]	Okereke HC & Kanu I. Laboratory Guide in Microbiology: Identification of bacterial pathogens. 2nd Edition, (Editors-Onyeagba and Isuh) Crystal Publishers, Okigwe-Nigeria 2004, PP. 34-44.
[12]	Cheesbrough M. District laboratory practice in tropical countries, part 2. Cambridge University Press, Cambridge, UK 2006, PP. 137-150.
[13]	NCCLS, National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 6th ed. Approved standard, Wayne, Pennsylvania 2003, PP. M7-A6.
[14]	Olukoya DK & Oni A. Plasmid profile analysis and antibiotics susceptibility patterns of Shigella isolates from Nigeria. Epidemiological Infections 1990, 105: 59-64.
[15]	[15] Yah SC, Eghafona NO, Enabulele IO & Aluyi HAS. Ampicillin usage and ampicillin resistant plasmids mediated Escherichia coli isolated from diarrhoeagenic patients attending some teaching hospital in Nigeria. Shiraz E-Medical Journal 2006, 7(4): 1-12.
[16]	Shittu AO, Kolawole OD & Oyedepo RE. Wound infections in two health institutions in Ile-Ife, Nigeria: Results of a cohort study. Osteology Wound Management 2004, 49(5): 52-57.
[17]	Alghalibi SMS, Humaid AA, Alshaibani EAS & Alhamzy EHL. Microorganisms Associated With Burn Wound Infection in Sana’a, Yemen. Egyptian Academic Journal of biological Sciences 2011, 3(1): 19-25.
[18]	Sani RA, Garba S, Oyewole OA & Ibrahim A. Antibiotic resistance profile of gram positive bacteria isolated from wound infections in Minna, Bida, Kontagora and Suleja Area of Niger State. Journal of Health Sciences 2012, 2(3): 19-22.
[19]	Tran TS, Jamulitrat S, Chongsuvivatvong V & Geater A. Prospective hospital acquired infection in Hungvuong Obstetrics and Gynaecological Hospital, Vietnam. Journal of Hospital Infections 1998, 40: 141-147.
[20]	Emele FE, Izomoh MI & Alufohai E. Microorganisms associated with wound infections in Ekpoma, Nigeria. West African Journal of Medicine 1999, 18(2): 97-100.
[21]	Mashita K, Shinagawa N & Sato T. Bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents. Japanese Journal of Microbiology 2000, 53 (80): 533-565.
[22]	[22] Rastegar A, Alaghehbandan R & Akhlaghi L. Burn wound infection and antimicrobial resistance in Tehran, Iran: An increasing problem. Ann Burn Fire Disasters 2005, 18: 1115-1118.
[23]	Nwachukwu NC, Orji FA & Okike UM. Antibiotic Susceptibility patterns of bacterial isolates from surgical wounds in Abia State University Teaching Hospital (ABSUTH), Aba – Nigeria. Research Journal of Medicine and Medical Sciences 2009, 4(2): 575-579.
[24]	Ohalete CN, Obi RK & EmeaKoroha MC. Bacteriology of different wound infection and their antimicrobial susceptibility patterns in Imo State Nigeria. World Journal of Pharmacy and Pharmaceutical Sciences 2012, 1(3): 1155-1172.
[25]	Peacock SJ, DeSilva I & Lowy FD. What determines nasal carriage of Staphylococcus aureus? Trends Microbiology 2001, 9: 605–610.
[26]	Barkocy-Gallagher G, A, Arthur T, Rivera-betancourt M, Nou X, Shackelford SD, Wheeler TL & Koohmaraie, M. Seasonal Prevalence of Shiga Toxin–Producing Escherichia coli, Including O157:H7 and Non-O157 serotypes, and Salmonella in commercial beef processing plants. Journal of Food Protection 2003, 66 (11): 1978–1986.
[27]	Etok CA, Edem EN & Ochang E. Aetiology and antimicrobial studies of surgical wound infections in University of Uyo Teaching Hospital (UUTH) Uyo, Akwa Ibom State, Nigeria. Open Access Scientific Reports 2012, 1(7): 1-5.
[28]	Udoh DI & Njirinze CI. In vitro analysis on antibiotic susceptibility patterns of some bacterial isolates from patients with genitourinary tract infections in Uyo, Akwa-Ibom State, Nigeria. International Journal of Biotechnology and Allied Sciences 2007, 2(3): 266-271.
[29]	Tillotson GS, Draghi DC, Sahm DF, Tomfohrde KM, Fabro T & Critchley IA. Susceptibility of Staphylococcus aureus isolated from skin and wound infections in the United States 2005–07: laboratory-based surveillance study. Journal of Antimicrobial Chemotherapy 2008, 62: 109–115.
[30]	Chigbu CO & Ezeronye OU Antibiotic resistance patterns of Hospital strains of Staphylococcus aureus isolated from ear and nasal carriers among students of Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria. African Journal of Biotechnology 2003, 2(10): 374-378.
[31]	Obajuluwa AF, Onaolapo JA, Oyi AR & Olayinka BO. Susceptibility profile of Methicillin-Resistant Staphylococcus aureus (MRSA) isolates to antibiotics and methanolic extracts of Parkia biglobosa (Jacq) Benth. British Journal of Pharmaceutical Research2013, 3(4): 587-596.
[32]	Uwaezuoke JC & Aririatu LE. A survey of Antibiotic resistant Staphylococcus aureus strains from clinical specimen. Journal of Applied Sciences and Environmental Management 2004, 8: 67-69.
[33]	Masaadeh HA & Jaran AS. Incident of Pseudomonas aeruginosa in post-operative wound infection. American Journal of Infectious Diseases 2009, 5 (1): 1-6.
[34]	Anjum F & Mir A. Susceptibility pattern of Pseudomonas aeruginosa against various antibiotics. African Journal of Microbiology Research 2010, 4 (10): 1005-1012.
[35]	Prescott LM, Harley JP & Klein DA. Microbiology. Sixth edition. McGraw Hill Publishers, New York, USA 2005, PP. 52, 288-291, 514, 792-793.
[36]	Nester EW, Anderson DG, Roberts CE, Pearsall NN & Nester MT. Microbiology: A human perspective. Fourth edition. McGraw Hill Companies Inc., New York, USA 2004, PP. 209-212, 691-698.
[37]	Anyim C, Nworie O, Onwa NC, Agah MV & Ugwu EN. Plasmid profile of Escherichia coli, Staphylococcus aureus and Streptococcus pneumoniae Isolated from sputum of paragonimiasis patients in Afikpo South, Ebonyi – Nigeria. African Journal of Sciences 2010, 11(1): 2646 – 2656.
[38]	Mark H, Donnak C, James RI, Allen K, Teng-Yung F & Benjamin V. Plasmids in diatoms species. Journal of Bacteriology 1991, 178(18): 5924-5927.

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. (2013). Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. American Journal of Bioscience and Bioengineering, 1(6), 75-82. https://doi.org/10.11648/j.bio.20130106.11

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Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am. J. BioSci. Bioeng. 2013, 1(6), 75-82. doi: 10.11648/j.bio.20130106.11

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am J BioSci Bioeng. 2013;1(6):75-82. doi: 10.11648/j.bio.20130106.11

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@article{10.11648/j.bio.20130106.11,
  author = {Nworie O. and Nnachi A. U. and Ukaegbu C. O and Alo M. N. and Ekuma U. O. and Ogueji E. O.},
  title = {Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {1},
  number = {6},
  pages = {75-82},
  doi = {10.11648/j.bio.20130106.11},
  url = {https://doi.org/10.11648/j.bio.20130106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20130106.11},
  abstract = {Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.},
 year = {2013}
}

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TY - JOUR
T1 - Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State
AU - Nworie O.
AU - Nnachi A. U.
AU - Ukaegbu C. O
AU - Alo M. N.
AU - Ekuma U. O.
AU - Ogueji E. O.
Y1 - 2013/10/20
PY - 2013
N1 - https://doi.org/10.11648/j.bio.20130106.11
DO - 10.11648/j.bio.20130106.11
T2 - American Journal of Bioscience and Bioengineering
JF - American Journal of Bioscience and Bioengineering
JO - American Journal of Bioscience and Bioengineering
SP - 75
EP - 82
PB - Science Publishing Group
SN - 2328-5893
UR - https://doi.org/10.11648/j.bio.20130106.11
AB - Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.
VL - 1
IS - 6
ER -

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Author Information

Nworie O.

Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State
Nnachi A. U.

Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ukaegbu C. O

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Plateau State
Alo M. N.

Department of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Awka, Anambra State
Ekuma U. O.

Department of Applied Microbiology, Faculty of Biological Science, Ebonyi State University, Abakaliki, Ebonyi State
Ogueji E. O.

Department of Biological Science, Faculty of Science and Technology, Federal University Ndufu-Alike Ikwo, Ebonyi State

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. (2013). Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. American Journal of Bioscience and Bioengineering, 1(6), 75-82. https://doi.org/10.11648/j.bio.20130106.11

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Nworie O.; Nnachi A. U.; Ukaegbu C. O; Alo M. N.; Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am. J. BioSci. Bioeng. 2013, 1(6), 75-82. doi: 10.11648/j.bio.20130106.11

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Nworie O., Nnachi A. U., Ukaegbu C. O, Alo M. N., Ekuma U. O., et al. Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State. Am J BioSci Bioeng. 2013;1(6):75-82. doi: 10.11648/j.bio.20130106.11

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@article{10.11648/j.bio.20130106.11,
  author = {Nworie O. and Nnachi A. U. and Ukaegbu C. O and Alo M. N. and Ekuma U. O. and Ogueji E. O.},
  title = {Antibiotic Susceptibility and Plasmid Profile of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli Isolated from Wound Patients in Abakaliki Metropolis, Ebonyi State},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {1},
  number = {6},
  pages = {75-82},
  doi = {10.11648/j.bio.20130106.11},
  url = {https://doi.org/10.11648/j.bio.20130106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20130106.11},
  abstract = {Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli has become increasingly recognized as an emerging opportunistic pathogen of clinical importance. A total of 18 isolates comprising of 10 (55.6%) S. aureus, 6 (33.3%) P. aeruginosa and 2 (11.1%) E. coli were recovered from 15 pus samples of wound patients attending Federal Teaching Hospital Abakaliki I (FETHA I) and Federal Teaching Hospital Abakaliki II (FETHA II). All the isolates were subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method on Mueller-Hinton agar. S. aureus showed the highest susceptibility percentage of 80.0% to ciprofloxacin, followed by 60.0% to augmentin and 50.0% to streptomycin, while the highest resistance percentage was obtained with lincocin (100.0%), followed by ampiclox (80.0%). P. aeruginosa showed the highest susceptibility percentage of 83.3% to ciprofloxacin, followed by 66.6% to streptomycin and gentamycin (66.6%), while the highest resistance percentage was obtained with streptomycin (33.3%) and gentamycin (33.3%). E. coli showed the highest susceptibility percentage to gentamycin and streptomycin with 100% activity. The antibiotics with reasonable resistant profile was observed in 10 isolates (5 S. aureus, 5 P. aeruginosa and 1 E. coli) with isolate code S1, S2, S3, S4, S5, Ps1, Ps2, Ps3, Ps4, Ps5 and Ec1 which showed resistance to atleast 5 antibiotics, hence this isolates were subjected to plasmid profile analysis. Only three isolates (S1, S4 and Ec1) showed the presence of plasmids within the range of 1.8 kbp to 10.4 kbp. Hence antibiotic resistance of an organism does not always confer the presence of plasmid.},
 year = {2013}
}

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