Rock Phosphate Solubilisation by Strains of Penicillium Spp. Isolated from Farm and Forest Soils of three Agro Ecological Zones of Cameroon
American Journal of Agriculture and Forestry
Volume 2, Issue 2, March 2014, Pages: 25-32
Received: Jan. 24, 2014;
Published: Feb. 28, 2014
Views 3141 Downloads 213
Fankem Henri, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon
Ngo Nkot Laurette, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon
Nguesseu Njanjouo Ghislain, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon
Tchuisseu Tchakounte Gylaine Vanessa, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon
Tchiazé Ifoué Alice Virginie, Department of Plant Biology, Faculty of Science, University of Douala, Douala, Cameroon
Nwaga Dieudonné, Department of Plant Biology and Physiology, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon
Rock Phosphate (RP) is theoretically the cheapest fertilizer and most abundant, but its direct application in the soils is not always agronomically effective due to its low phosphorus (P) availability. An environmentally friendly and economically alternative to traditional processing of these deposits is the use of Phosphate Solubilising Microorganisms (PSM). Three Penicillium strains were screened for their aptitude in solubilising sparingly soluble phosphates as well as phosphate rock on plates and in broth supplemented with the different phosphate types. The three strains show the ability in solubilising at least two from the sparingly soluble phosphates types, with the solubilisation index (SI) varying from 1.44 to 4.74. All the strains were able to show halo zone on plates supplemented with Malian and Algerian rock phosphates with solubilisation index ranging from 1.2 to 1.8. No halo zone was detected on plates with Moroccan rock phosphate as sole phosphate source. However, significant amount of phosphorus were mobilised by all strains in broth with the different rock phosphates, including the Moroccan rock phosphate. The amount of solved P varied from 257.46 mgP.L-1 (Penicillium sp. 43) to 326.65 mgP.L-1 (Penicillium sp. 109) for Malian rock phosphate, from 152.61 mgP.L-1 (Penicillium sp. 43) to 337.56 mgP.L-1 (Penicillium sp. 27) for Algerian rock phosphate, and from 283.79 mgP.L-1 (Penicillium sp. 43) to 347.16 mgP.L-1 (Penicillium sp. 109) for Moroccan rock phosphate. The rock phosphate solubilisation was associated with pH media drop and both parameters were strongly correlated.
Ngo Nkot Laurette,
Nguesseu Njanjouo Ghislain,
Tchuisseu Tchakounte Gylaine Vanessa,
Tchiazé Ifoué Alice Virginie,
Rock Phosphate Solubilisation by Strains of Penicillium Spp. Isolated from Farm and Forest Soils of three Agro Ecological Zones of Cameroon, American Journal of Agriculture and Forestry.
Vol. 2, No. 2,
2014, pp. 25-32.
S.S.S. Rajan, J.H. Watkinson, A.G. Sinclair, Phosphate rocks for direct application to soils, vol. 57. Adv. Agron. 1996, pp. 77-159.
H.T. Rogers, R.W. Pearson, L.E. Ensminger, Comparative efficiency of various phosphate fertilizers, vol. 4. Agronomy, 1953, pp. 189-242.
G.W. Cooke, The value of rock phosphate for direct application, vol. 24. Empire Journal of Experimental Agriculture 1956, pp. 295-306.
A.H. Babana, A. H. Dicko, K. Maïga and D. Traoré, Characterization of rock phosphate-solubilizing microorganisms isolated from wheat (Triticum aestivum L.) rhizosphere in Mali, vol. 1(1). Journal of Mıcrobıology and Mıcrobıal Research 2013, pp. 1-6.
A.C. Gaur, & K.P. Ostwall, Infuence of phosphate dissolving bacilli on yield and phosphate uptake of wheat crop, vol. 10. Indian Journal of Experimental Biology 1972, pp. 393-394.
A.H. Babana, H. Antoun, Effect of Tilemsi phosphate rock solubilizing microorganisms on phosphorus-uptake and yield of field grown wheat in Mali, vol. 287. Plant and Soil, 2006, pp. 51-58.
H. Hamdali, K. Moursalou, G. Tchangbedji, Y. Ouhdouch, H. Mohamed, Isolation and characterization of rock phosphate solubilizing actinobacteria from a Togolese phosphate mine, vol. 11(2). Afr. J. Biotechnol. 2012, pp. 312-320.
Varsha Narsian, H.H. Patel, Aspergillus aculeatus as a rock phosphate solubilizer, vol. 32. Soil Biology & Biochemistry, 2000, pp. 559-565.
R.S. Gadagi, T. Sa, New Isolation Method for Microorganisms Solubilizing Iron and Aluminium Phosphates Using Dyes, vol. 48(4). Soil Science and Plant Nutrition, 2002, pp. 615-618.
M.A. Qureshi, Z.A. Ahmad, N. Akhtar, A. Iqbal, F. Mujeeb, M.A. Shakir, Role of phosphate solubilizing bacteria (PSB) in enhancing P availability and promoting cotton growth, vol. 22. The journal of animal & plant sciences, 2012, pp. 204-210.
C.S. Nautiyal, An efficient microbiological growth medium for screening phosphate solubilizing microorganisms, vol. 170. FEMS Microbiology Letters, 1999, pp. 265-270.
S. Mehta, S.C. Nautiyal, An efficient method for qualitative screening of phosphate-solubilizing bacteria, vol. 43. Current Microbiol., 2001, pp. 51-56.
H. Fankem, M. Abba, L. Ngo Nkot, A. Deubel, W. Merbach, F.X. Etoa and D. Nwaga, "Selecting indigenous P solubilizing bacteria for cowpea and millet improvement in nutrient deficient acidic soils of southern Cameroon". In Innovations for a green revolution in Africa: Exploring the Scientific Facts. A. Bationo, B. Waswa, J. Okeyo, F. Maina (Eds.). Springer Science+Business Media B.V. 2011 (DOI 10.1007/978-90-481-2543-2_40), pp. 391-398.
L.A. Fernandez, P. Zalba, M.A. Gomez, M.A. Sagardoy, Phosphate solubilization activity of bacterial strains in soil and their effect on soybean growth under greenhouse conditions, vol. 43. Biol. Fert. Soils, 2007, pp. 805-809.
Reyes, R. Baziramakenga, L. Bernier, and H. Antoun, Solubilization of phosphate rocks and minerals by a wild-type strain and two UV-induced mutants of Penicillium rugulosum, vol. 33. Soil Biology and Biochemistry, 2001, pp. 1741-1747.
Reyes, L. Bernier, H. Antoun, Rock phosphate solubilization and colonization of maize rhizosphere by wild and genetically modified strains of Penicillum rigulosum, vol. 44. Microbial Ecology, 2002, pp. 39-48.
S.A. Wakelin, R.A. Warren, P.R. Harvey, M.H. Ryder, Phosphate solubilization by Penicillium spp. closely associated with wheat roots, vol. 40. Biology and Fertility of Soils, 2004, pp. 36-43.
A. Deubel, W. Merbach, "Influence of microorganisms on phosphorus bioavailability in soils". In Microorganisms in soils: roles in genesis and functions. F. Buscot, A. Varma (eds). Springer, Berlin Heidelberg, 2005, pp. 177–191.
P.E.A. Asea, R.M.N. Kucey, J.W.B. Stewart, Inorganic phosphate solubilization by two Penicillium species in solution culture and in soil, vol. 20. Soil Biology & Biochemistry, 1988, pp. 459-464.
S.A. Omar, The role of rock-phosphate-solubilizing fungi and vesicular-arbusular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate vol. 14. World Journal of Microbiology & Biotechnology, 1988, pp. 211-218.
S.L. Yu, Y.N. Liu, G.L. Jing, B.J. Zhao, S.Y. Guo, Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase reaction-denaturing gradient gel electrophoresis assay, vol. 17. J. Environ. Sci., 2005, pp. 611–614.
G.N. Silva, C. Vidor, Phosphate solubilising activity of microorganisms in the presence of nitrogen, iron, calcium and potassium, vol. 36. Pestic Agro Bras., 2001, pp. 1495–1508