The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.
| Published in | American Journal of Agriculture and Forestry (Volume 2, Issue 5) |
| DOI | 10.11648/j.ajaf.20140205.11 |
| Page(s) | 209-218 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
PRSV-P, Coat protein, Phylogeny, Papaya, Squash, Symptom intensity
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APA Style
Douglas Rodríguez Martínez, Priscilla de Sousa Geraldino Duarte, Justo González Olmedo, Antonia dos Reis Figueira. (2014). Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. American Journal of Agriculture and Forestry, 2(5), 209-218. https://doi.org/10.11648/j.ajaf.20140205.11
ACS Style
Douglas Rodríguez Martínez; Priscilla de Sousa Geraldino Duarte; Justo González Olmedo; Antonia dos Reis Figueira. Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. Am. J. Agric. For. 2014, 2(5), 209-218. doi: 10.11648/j.ajaf.20140205.11
AMA Style
Douglas Rodríguez Martínez, Priscilla de Sousa Geraldino Duarte, Justo González Olmedo, Antonia dos Reis Figueira. Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba. Am J Agric For. 2014;2(5):209-218. doi: 10.11648/j.ajaf.20140205.11
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Download@article{10.11648/j.ajaf.20140205.11,
author = {Douglas Rodríguez Martínez and Priscilla de Sousa Geraldino Duarte and Justo González Olmedo and Antonia dos Reis Figueira},
title = {Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba},
journal = {American Journal of Agriculture and Forestry},
volume = {2},
number = {5},
pages = {209-218},
doi = {10.11648/j.ajaf.20140205.11},
url = {https://doi.org/10.11648/j.ajaf.20140205.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20140205.11},
abstract = {The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country.},
year = {2014}
}
TY - JOUR T1 - Molecular and Biological Studies of Papaya Ringspot Virus Isolates from Brazil and Cuba AU - Douglas Rodríguez Martínez AU - Priscilla de Sousa Geraldino Duarte AU - Justo González Olmedo AU - Antonia dos Reis Figueira Y1 - 2014/08/30 PY - 2014 N1 - https://doi.org/10.11648/j.ajaf.20140205.11 DO - 10.11648/j.ajaf.20140205.11 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 209 EP - 218 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20140205.11 AB - The coat protein genes of 21 Papaya ringspot virus (PRSV) isolates from Brazil and 7 isolates from Cuba were sequenced and analyzed, and the symptoms induced by the virus isolates were evaluated in papaya (Carica papaya L. cv. Solo) and squash (Cucurbita pepo L. cv. Caserta). The virus isolates were grouped in phylogenetic trees by geographic region, presenting the highest nucleotide and amino acid identities with those isolates collected in the same regions. The American and Indian isolates, from GenBank were grouped together in the tree, and the Brazilian isolates were grouped by state; the Cuban isolates from the eastern region were closer to the American isolates than to those from the central-west region. Typical mosaic symptoms, grouped according to intensity, were observed in the inoculated papaya and squash seedlings. The variability of the coat protein genes confirmed that virus control using cross-protection and transgenic plants requires the selection of region-specific virus isolates in each country. VL - 2 IS - 5 ER -
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