Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers
American Journal of Agriculture and Forestry
Volume 4, Issue 4, July 2016, Pages: 86-96
Received: May 25, 2016; Accepted: Jun. 12, 2016; Published: Jul. 23, 2016
Views 3624      Downloads 135
Cosmas Sorngmenenye Abengmeneng, Department of Silviculture and Forest Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Daniel Ofori, Forestry Research Institute of Ghana, Council for Scientific and Industrial Research, Kumasi, Ghana
Philomena Kumapley, Department of Silviculture and Forest Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Richard Akromah, Department of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Ramni Jamnadass, International Centre for Research into Agroforestry, Nairobi, Kenya
Marian Quain, Crop Research Institute, Council for Scientific and Industrial Research, Kumasi, Ghana
Article Tools
Follow on us
Ceiba pentandra (L.) Gaertn (trade name Ceiba) of the family Bombacaceae is an important multi-purpose tree species in Ghana and demand for it is rising daily. As a result, it has been included as one of the species for the National Forest Plantation Development Programme of Ghana as part of the efforts towards its restoration. Seedlings for the programme are being raised from seed collected from the wild without regard for their genetic quality and its future adverse effects. The absence of adequate information on the genetic diversity within the species therefore serves as a potential threat to its long term sustainable management and efficient genetic conversation. The phylogenetic relationships of 36 genotypes of C. pentandra from natural range of the species in five ecological zones (populations) of Ghana were therefore analyzed using 10 polymerase chain reaction (PCR) markers (5 random amplified polymorphic DNAs (RAPDs) and 5 inter simple sequence repeats (ISSRs)). The principal component analysis (PcoA) defined by axis 1 and 2 accounted for 67.15% of the variation observed. Cluster analysis using GeneStat Discovery showed that, 14 (38.89%), 21 (58.33%) and 4 (11.11%) of the accessions had a coefficient of similarity of 1 from the RAPD, ISSR and the combined RAPD and ISSR polymorphism respectively. The most distantly related accessions from the RAPD polymorphism analysis were BAW 1 and KON 8 with a similarity coefficient of 0.06 whereas those from the ISSR polymorphism were BAW 10 and ASE 1 with 21% similarity between them. Similarly, BUF 1 and KUE 1 were the most dissimilar accessions from the combined RAPD and ISSR fragments analysis with a similarity coefficient of 0.23. Nine (25%) accessions with high degree of dissimilarity between them were identified in the study. These accessions could serve as good candidates for conservation as seed trees and in breeding programs of C. pentandra in Ghana. Implications of the findings for effective management of the genetic resources of the species were also discussed.
Ceiba pentandra (L.), ISSR, RAPD, Genetic Diversity, Population, Genotypes
To cite this article
Cosmas Sorngmenenye Abengmeneng, Daniel Ofori, Philomena Kumapley, Richard Akromah, Ramni Jamnadass, Marian Quain, Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers, American Journal of Agriculture and Forestry. Vol. 4, No. 4, 2016, pp. 86-96. doi: 10.11648/j.ajaf.20160404.13
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Burkill HM, 1985 The Useful Plants of West Africa. Second edition. Royal Botanic Gardens, Kew. Pp. 278-283.
Irvine FR, 1961 Woody Plants of Ghana with Special Reference to their Uses. Oxford University Press. Pg. 191.
Siepel A, Poorter L, Hawthorne WD, 2004 Ecological Profiles of large timber species. In: Biodiversity of West African Forests. Poorter L, Bongers F, Kouame FN, Hawthorne WD (editors). CABI Publishing, Wallingford, UK. Pp 403.
Lobo JA, Quesada M, Stoner KE, 2005 Effects of pollination by bats on the mating system of Ceiba pentandra (Bombacaceae) populations in two tropical life zones in Costa Rica. American Journal of Botany 92: 370-376. DOI: 10.3732/ajb.92.2.370
Dick CW, Birmingham E, Lemes MR, Gribel R, 2007 Extreme long-distance dispersal of the lowland tropical rainforest tree Ceiba pentandra L. (Malvaceae) in Africa and the Neotropics. Molecular Ecology 16: 3039-3049.
Brondani RPV, Gaiotto FA, Missiaggia AA, Kirst M, Gribel R, Grattapaglia D, 2003 Microsatellite markers for Ceiba pentandra (Bombacaceae), an endangered tree species of the Amazon forest. Molecular Ecology Notes 3: 177-179. DOI: 10.1046/j.1471-8286.2003.00389.x
Williams JW, Jackson ST, Kutzbach JE, 2007 Projected distributions of novel and disappearing climates by 2100 AD. Proceedings of the National Academy of Science, USA 104: 5738-5742.
Gillet E, Gomory D, Paule L, 2005 Measuring genetic variation within and among populations at marker loci. In Conservation and Management of Forest Genetic Resources in Europe. Geburek T, Turok J (editors). Arbora Publishers, Zvolen. Pp 237-273.
Vendramin GG, Hansen OK, 2005 Molecular markers for characterizing diversity in forest trees. In Conservation and Management of Forest Genetic Resources in Europe. Geburek T, Turok J (editors). Arbora Publishers, Zvolen. Pp 337-368
Archak S, Gaikwad AB, Gautam D, Rao EVVB, Swamy KRM, Karihaloo JL, 2003 Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India. Genome 46: 362-369.
Ofori DA, 2001 Genetic diversity and its implications for the management and conservation of Milicia species. PhD thesis, University of Aberdeen. Pp 47.
Enaberuel LO, Obisesan IO, Okolo EC, Akinwale RO, Aisueni NO, Ataga CD, 2014 Genetic Diversity of Shea Butter Tree (Vitellaria paradoxa C. F. Gaernt) in the Guinea Savanna of Nigeria Based on Morphological Markers. American-Eurasian J. Agric. & Environ. Sci., 14 (7): 615-623. DOI: 10.5829/idosi.aejaes.2014.14.07.12365
Grattapaglia D, 2007 Marker-assisted selection in Eucalyptus. In: Current status and future perspectives in crops, livestock, forestry and fish. Guimaraes EP, Ruane J, Scherf BD, Sonnino A, Dargie JD (editors). Food and Agriculture Organization of the United Nations, Rome. Pp 252-281.
Deng C, Zhou J, Gao W, Sun F, Qin R, Lu L, 2006 Assessment of genetic diversity of Lycoris longituba (Amaryllidaceae) detected by RAPDs. Journal of Genetics 85 (3): 205-207.
Monteleone I, Ferrazzini D, Belletti P, 2006 Effectiveness of neutral RAPD markers to detect genetic divergence between the subspecies uncinata and mugo of Pinus mugo Turra. Silva Fennica 40 (3): 391-406.
Brown JKM, 1996 The choice of molecular marker methods for population genetics studies of plant pathogens. New Phytopathology 133: 183-195.
Xia T, Meng L, Mao K, Tian B, Miehe G, Liu J, 2008. Genetic variation in the Qinghai-Tibetan plateau endemic and endangered conifer Cupressus gigantea, detected using RAPD and ISSR markers. Silvae Genetica 57 (2): 85-92.
Lee S, Kim Y, Kim W, 2003 Lack of allozyme and ISSR variation in the Rare endemic tree species, Berchemia berchemiaefolia (Rhamnaceae) in Korea. Annals of Forest Science 60: 357–360 357.
Gupta S, Srivastava M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, Kumar M, Singh R, 2008 Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. African Journal of Biotechnology 7 (23): 4230-4243.
Egnin M, Mora A, Prakash CS, 1998 Factors enhancing Agrobacterium tumefaciens – Mediated Gene Transfer in peanut (Arachis hypogeal L.). In vitro Cellualar and Developmental Biology of Plants, 34: 310-318.
Nei M, 1972 Genetic distance between populations. American Naturalist, 106: 283-292.
Nei M, Li W, 1979 Mathematical model for studying genetical variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76 (10): 5269-5273.
Abengmeneng C S, Ofori DA, Kumapley P, Akromah R, Jamnadass R, 2015 Estimation of heritability and genetic gain in height growth in Ceiba pentandra. African Journal of Biotechnology, 14 (22): 1880-1885. DOI: 10.5897/AJB2014.14287
Stacy EA, 2001 Cross-fertility in two tropical tree species: evidence of inbreeding depression within populations and genetic divergence among populations. American Journal of Botany, 88: 1041-1051.
El-Kassaby YA, Cook C, 1994 Female reproductive energy and reproductive success in a Douglas-fir seed orchard and its impact on genetic diversity. Silvae Genetica, 43: 243-246.
Boshier DH, 2000 Mating systems. In: Forest conservation genetics: principles and practice. Young A, Boshier D, Boyle T (editors). CSIRO Publishing/CABI Publishing, Melbourne/Wallingford. Pp 63-79
Hansson B, Westerberg L, 2002 On the correlation between heterozygosity and fitness in natural populations. Molecular Ecology 11: 2467-2474.
Uyoh EA, Umego C, Aikpokpodion PO, 2014 Genetic diversity in African Nutmeg (Monodora myristica) accessions from South Eastern Nigeria. African Journal of Biotechnology, 13 (42): 4105-4111. DOI: 10.5897/AJB2014.14075
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186