Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry
American Journal of Agriculture and Forestry
Volume 4, Issue 6, November 2016, Pages: 156-162
Received: Dec. 6, 2016; Published: Dec. 7, 2016
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Authors
Xingjun Xi, Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, China
Shangzhen Xiao, Chemical Processing Engineering of Forest Products, Beijing Forestry University, Beijing, China
Fei Tang, Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, China
Qiao Chu, Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, China
Tao Lan, Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, China
Genlai Dong, Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, China
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Abstract
To obtain monosaccharide components information of Dendrobium moniliforme polysaccharides, a HPLC-ESI-MS method was proposed. The crude polysaccharides were extracted and purified from D. moniliforme as samples (DMP). Then, the polysaccharides were hydrolyzed with trifluoroacetic acid (TFA) and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-labeled mixture of monosaccharides were separated by a reverse-phase high performance liquid chromatography (HPLC) and detected by on-line electrospray ionization mass spectrometry (ESI-MS). With this method, the six monosaccharide derivatives have been well separated and the identification of the monosaccharides composition was carried out. The result showed that D. moniliforme polysaccharide consisted of D-mannose, D-ribose, D-glucose, D-galactose and L-arabinose, in a molar ratio of 51.83: 4.01: 38.90: 4.20: 1.65, respectively. Moreover, hydrolysis studies revealed that the monosaccharide concentrations reached the peak value at different time and it suggested that the release of DMP monosaccharides was not hydrolyzed at the same rate. These results indicated that the DMP may be a mixture of multiple polysaccharides. Finally, we used a high performance gel permeation chromatography (HPGPC) method proved that crude polysaccharides were composed by three kinds of polysaccharides (DMP-1, DMP-2 and DMP-3) with molecular weight of 153.1 kDa, 69.28 kDa and 50.14 kDa.
Keywords
Dendrobium moniliforme Polysaccharide, Monosaccharide Components, High Performance Gel Permeation Chromatography (HPGPC), High-Performance Liquid Chromatography (HPLC), Electronspray Ionization-Mass Spectroscopy (ESI-MS)
To cite this article
Xingjun Xi, Shangzhen Xiao, Fei Tang, Qiao Chu, Tao Lan, Genlai Dong, Monosaccharide Analysis of Dendrobium moniliforme Polysaccharides by High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry, American Journal of Agriculture and Forestry. Vol. 4, No. 6, 2016, pp. 156-162. doi: 10.11648/j.ajaf.20160406.13
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