Plant Growth Regulators Affect Germination and Main Carbon-Nitrogen Metabolites of Potato Tubers
American Journal of Agriculture and Forestry
Volume 7, Issue 1, January 2019, Pages: 10-16
Received: Nov. 6, 2018;
Accepted: Dec. 29, 2018;
Published: Jan. 30, 2019
Views 355 Downloads 54
Zhong Lei, Key Laboratory of Crop Ecophysiology and Farming System in Southwest of China, Ministry of Agriculture/College of Agronomy, Sichuan Agricultural University, Chengdu, China
Wang Liangjun, Agricultural Extension Station of Dongpo District of Meishan City in Sichuan, Meishan, China
Yuan Jichao, Key Laboratory of Crop Ecophysiology and Farming System in Southwest of China, Ministry of Agriculture/College of Agronomy, Sichuan Agricultural University, Chengdu, China
Zheng Shunlin, Key Laboratory of Crop Ecophysiology and Farming System in Southwest of China, Ministry of Agriculture/College of Agronomy, Sichuan Agricultural University, Chengdu, China; Ministry of Agriculture Key Laboratory of Tuber Crop Genetics and Breeding, Chengdu Jiusen Agricultural, Chengdu, China
Hu Jianjun, Crop Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
Assessing the effects of different growth regulators on sprout growth and tuber carbon-nitrogen metabolism during the storage of potato tubers is helpful to understand the physiological changes of dormancy in potatoes. In this study, a major potato cultivar ‘Chuanyu-117’ of Southwestern China was used as the experimental material. The tubers were immersed in sprouting inhibitors chlorpropham (CIPC), abscisic acid (ABA), and gibberellin A3 (GA3) to study sprout growth and changes in the carbon-nitrogen metabolism during tuber storage. The results showed that GA3 treatment shortened the dormancy intensity and amplitude by 17 days and 11 days, respectively, and that the sprouts grew faster after germination. Compared to the control treatment, ABA prolonged dormancy and increased its intensity by 6 days but shortened the amplitude by 11 days. After dormancy, the sprouts grew rapidly and uniformly. The CIPC treatment had a more significant dormancy prolonging effect. The stems germinated 70 days post treatment, and the germination period was extended by at least 40 days compared with the control. Tubers treated with different growth regulators showed similar carbon-nitrogen metabolism during the storage period. However, the rate of variation was significantly different. After 12 weeks, the starch content of tubers treated with GA3, ABA, and CIPC decreased by 13.36%, 11.30%, and 5.93%, respectively, while soluble sugar content decreased by 48.3%, 58.9%, and 56.1%, respectively. Soluble protein and crude protein content in the treated tubers first increased and then decreased. Soluble protein content in the tubers treated with GA3, ABA, and CIPC increased during storage but decreased in the later period. Crude protein content also increased the first time, and then decreased in the later period. Changes in the carbon-nitrogen ratio of tubers in different treatments during storage were also different. The carbon-nitrogen ratio of tubers treated with sprouting promotors was high, while that of the tubers treated with sprouting inhibitors was low and showed a gradual decreasing trend.
Plant Growth Regulators Affect Germination and Main Carbon-Nitrogen Metabolites of Potato Tubers, American Journal of Agriculture and Forestry.
Vol. 7, No. 1,
2019, pp. 10-16.
Chen Y Y. Research of the content change of dry matter, reducing sugar and starch in potato in the storage period. Chinese Agricultural Science Bulletin, 2006.22(4):84-87. (In Chinese with English abstract).
Zhang W, Bai Y J, Li X Z, et al. Status quo and development trend of seed potato quality control. Chinese Potato Journal, 2010,24(3):186-189. (In Chinese with English abstract).
Kenneth W, Knutson. Implication of new technologies for seed potato certification programs and seed growers. American Potato Journal. 1988,65(4):229-235.
Yi J, Zhang X J, Ji L J, et al. How to realize potato’s safe storage and appreciation of production of our country. (In Chinese).
Zheng X H. Study on Mini Potato different storage method on seed vigor and physical and chemical properties. Gansu Agricultural University, 2010. (In Chinese with English abstract).
Deng C L. Potato dormancy and its breaking methods. Chinese Potato Journal, 2010,24(3):151-152. (In Chinese with English abstract).
Yang W L, Sui Q J. The dormancy characteristics of different gene type mini-tuber stuffs in potato breeding and their controlling. Southwest China Journal of Agricultural sciences, 2004,17(1):84-87. (In Chinese with English abstract).
Li P H. Effects of different GA3 concentration on potato dormancy. Guizhou Agricultural Sciences, 2008,36(3):34-36. (In Chinese with English abstract).
Zhong L, Deng J C, Wang L J, et al．Effect of plant growth regulators on germination and oxidase activity during storage of potato tubers. Acta Prataculturae Sinica,2017,(7):147-157. (In Chinese).
Gale E, Kleinkopf, Nathan A. Oberg, et al. Sprout inhibition in storage: current status, new chemistries and natural compounds. American Journal Of Potato Research,2003,80:317-327.
Elizabeth C. Schramm, Sven K. et al. Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar ‘Zak’. Theoretical and Applied Genetics,2013,126:791-803.
Chen Q B, Hou X L, Wang J J, et al. Effect of exogenous abscisic acid on the dormancy of onion (Allium cepa). Journal of Nanjing Agricultural University, 2007,30(1):30-33. (In Chinese with English abstract).
WANG C C，HU J J，JIA W W, et al. Effects of Gibberellin and Thiourea on Dormancy and Bud Morphogenesis of Pre-basic Potato under Different Tempertures. Southwest China Journal of Agricultural Sciences,2017,5:1035-1041. (In Chinese).
Liu F, Yang Y J, Dong D F, et al. Study on dormancy characteristics of potato with different generations and weight. Shandong Agricultural Sciences, 2011,11:32-34. (In Chinese with English abstract).
Xiong Q E. Plant physiology. Sichuan Science and Technology Press, 2003,81,82. (In Chinese).
Zhou W X, Dong P F, Wang X P, et al. Effect of low-light stress on kernel setting and metabolism of carbon and nitrogen in different maize (Zea mays L.) genotypes. Acta Agronomica Sinica, 2013,39(10):1826-1834. (In Chinese with English abstract).
Wu D P, Song M, Liu W B, et al. Advance in study on methods of maintaining and improving seed vigor. Seed, 2006,25(6):55-59. (In Chinese with English abstract).
Zhang L L, Chen Y L, Lian Y. The dormancy of potato tuber and the advances in study on dormancy regulation. Chinese Potato Journal, 2003,17(3):352-356. (In Chinese).
Luis Destefano-Beltran. Effects of postharvest storage and dormancy status on ABA content, metabolism and expression of genes involved in ABA biosynthesis and metabolism in potato tuber tissues. Plant Molecular Biology,2006,61:687-697.
Corsini D, Stallknecht G, Spark W. Changes in chlorpropham residues in stored potatoes. American Potato Journal.1979,56(1):43-50.
Huang Z, Tian S L, Ge X, , et al. Complexation of chlorpropham with hydroxypropyl-β-cyclodextrin and its application in potato sprout inhibition .Carbohydrate Polymers, Volume 107, 17 July 2014, Pages 241-246.
Liu L L, Li J, Li C H, et al. Evaluation of resistance level to late blight in the populations derived from the horizontal resistant crosses of potatoes. Chinese Potato Journal, 2004,18(4):201-204. (In Chinese with English abstract).
Sun M L, Yang W L, Li S L, et al. Study on potato dormancy characteristics and control of sprout. Chinese Agricultural Science Bulletin, 2004,20(6):81-84. (In Chinese with English abstract).
Sue Damon, John Hewitt, Matt Nieder et al. Sink Metabolism in tomato fruit. Plant Physiol,1988,87:731~736.
Zhu Z, Jiang J Y, Jiang C J, et al. Effects of low temperature stress on SOD activity, soluble protein content and soluble sugar content in Camellia sinensis leaves. Journal of Anhui Agricultural University, 2011,38(1):24-26. (In Chinese with English abstract).
Jiang Z J, Huang X P, Zhang J P. Effect of environmental stress on non-structural carbohydrates reserves and transfer in seagrasses. Acta Ecological Sinica, 2012,32(19):6242-6250. (In Chinese with English abstract).
Yang J, Shi Y W, Li Z W, et al. Advance in study on the carbon and nitrogen metabolism of rice. Crop Research, 2011,25(4):383-387. (In Chinese).
Hu Y Q, Zhan S, Ku W Z, et al. The Roles of ABA and GA3 in the Joint Regulation of Potato Tuber Formation and Starch Accumulation in Vitro. Molecular Plant Breeding, 2017,Vol.15, No.10, 4210-4214. (In Chinese with English abstract).