Spore Culture of Nephrolepis falcate (Cav). C. Chr.
American Journal of Agriculture and Forestry
Volume 3, Issue 6-1, December 2015, Pages: 10-14
Received: Mar. 28, 2015; Accepted: Jun. 30, 2015; Published: Nov. 10, 2015
Views 4535      Downloads 127
Author
Farida D. Silverio, Science Laboratory, College of Education, Cotabato Foundation College of Science and Technology, Arakan, Cotabato Philippines
Article Tools
Follow on us
Abstract
Spore culture of Nephrolepis falcate (Cav). C. Chr. was undertaken to propagate Nephrolepis falcata in vitro using the spores. Specifically, it aimed to find out the duration of the germination of Nephrolepis falcate spores and observe spore germination and prothallial development. Nephrolepis falcate is a fern plant under family Lomariopsidaceae which is found on shaded areas (Smith et al., 2006). Its rhizome is creeping, frond is simple pinnate, sori round and spores bilateral, monolete. This species was used as ornamental. Thus, there is a need to conduct an in vitro (spore) culture of this plant. In vitro culture of this species revealed a rapid spore germination of 3 days after inoculation. Germ filament formation of one to twelve cells was observed from 3rd to 16th day after inoculation, and prothallial plate formation was noticed 19th day after inoculation. Correspondingly, Nephrolepis falcate (Cav.) C. Chr. spore germination is of Vittaria type and its prothallial development follows Adiantum-type.
Keywords
Monolete, In Vitro, Germination, Prothallial Development, Inoculation, Spore Culture
To cite this article
Farida D. Silverio, Spore Culture of Nephrolepis falcate (Cav). C. Chr., American Journal of Agriculture and Forestry. Special Issue: Agro-Ecosystems. Vol. 3, No. 6-1, 2015, pp. 10-14. doi: 10.11648/j.ajaf.s.2015030601.13
References
[1]
Amoroso, V. B. 2006. Pteridophyte and Gymnosperm Diversity In Musuan, Bukidnon. Philippine Journal of Systematic Biology Vol. I, No.
[2]
Herrero, A., C. Prada and S. Pajarón. 2002. Gametophyte Morphology and Gametangial Ontogeny of Asplenium Foreziense and Related Taxa (Aspleniaceae: Pteridophyta). Botanical Journal of the Linnean Society, 139, 87–98.
[3]
Nayar, B. K. and S. Kaur. 1971. Gametophyte of Homosphorous ferns. Botanical Review. 37(3): 295 – 385.
[4]
Kramer, K. U., P. S. Green and E. Götz. 1990. Pteridophytes and Gymnosperms. 79 – 82.
[5]
Smith, A. R., K.M. Pryer, E. Schuettpelz, P. Korall, H. Schneider, and P.G. Wolf. 2006. A classification for extant ferns. TAXON 55(3): 705-731.
[6]
Amorsolo, C.B. 2009. Spore tissue culture of platycerium grande. Central Mindanao University Pamphlet. CMU University Town. Musuan, Bukidnon, Philippines.
[7]
Haddad, S. and R. Bayerly. 2014. In vitro propagation of ferns (Asplenium nidus) via spore culture. Jordan Journal of Agricultural Sciences. 10(1):144-153.
[8]
Bharati, S. K., D.C. Manabendra and M.P. Behari. 2013. In Vitro propagation in Pteridophytes: A Review. International J. Res. Ayurveda Pharm. 4(2):297-303.
[9]
Chang, H.C., D.C. Agrawal, C.L. Kuo, J.L. Wen, C. Chen and H.S. Tsay. In Vitro culture of Drynara fortune, a fern species source of chinese medicine “Gu-Sui-Bu”. In Vitro Cell. Dev. Biol. - Plant. 43(133-139.
[10]
Khan, S.M. Rasiq and H.A. Kagani. 2008. In Vitro propagation of birds Nest Fern (Asplenium nidus) from spores. Pak. J. Bot. 40(1): 91-97.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186