American Journal of BioScience

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Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology

Received: 23 October 2013    Accepted:     Published: 10 November 2013
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Abstract

The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.

DOI 10.11648/j.ajbio.20130104.12
Published in American Journal of BioScience (Volume 1, Issue 4, November 2013)
Page(s) 59-62
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Escherichia Coli, Oxidative Stress, Hydrogen Peroxide (H2O2), Cell Culturability, Cell Death

References
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Author Information
  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

Cite This Article
  • APA Style

    Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. (2013). Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. American Journal of BioScience, 1(4), 59-62. https://doi.org/10.11648/j.ajbio.20130104.12

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    ACS Style

    Md. Sakil Munna; Ifra Tun NUR; Tasmina Rahman; Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am. J. BioScience 2013, 1(4), 59-62. doi: 10.11648/j.ajbio.20130104.12

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    AMA Style

    Md. Sakil Munna, Ifra Tun NUR, Tasmina Rahman, Rashed Noor. Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology. Am J BioScience. 2013;1(4):59-62. doi: 10.11648/j.ajbio.20130104.12

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  • @article{10.11648/j.ajbio.20130104.12,
      author = {Md. Sakil Munna and Ifra Tun NUR and Tasmina Rahman and Rashed Noor},
      title = {Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology},
      journal = {American Journal of BioScience},
      volume = {1},
      number = {4},
      pages = {59-62},
      doi = {10.11648/j.ajbio.20130104.12},
      url = {https://doi.org/10.11648/j.ajbio.20130104.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbio.20130104.12},
      abstract = {The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.},
     year = {2013}
    }
    

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    T1  - Influence of Exogenous Oxidative Stress on Escherichia Coli Cell Growth, Viability and Morphology
    AU  - Md. Sakil Munna
    AU  - Ifra Tun NUR
    AU  - Tasmina Rahman
    AU  - Rashed Noor
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    DO  - 10.11648/j.ajbio.20130104.12
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 59
    EP  - 62
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20130104.12
    AB  - The present study attempted to determine the influence of exogenous oxidative stress on the cell viability of Escherichia coli. In this regard, 3mM hydrogen peroxide (H2O2) was added to the late log phase of E. coli culture, and afterwards the phenotype, cell morphology and the ability to form colony forming units (CFU) on agar plates were examined. As expected, a quick phenotypic suppression as well as a rapid decline in viable and culturable cell numbers was observed at the mid-stationary phase as compared to control. Interestingly, a large mass of cell aggregates was noticed upon addition of H2O2. Thus the current investigation corroborated the previous findings and further added to the existing knowledge on oxidative stress events in E. coli.
    VL  - 1
    IS  - 4
    ER  - 

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