A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis
American Journal of BioScience
Volume 2, Issue 3, May 2014, Pages: 84-88
Received: Apr. 7, 2014; Accepted: Apr. 15, 2014; Published: Apr. 30, 2014
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Hrachya Garegin Hovhannisyan, “Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia
Gohar Gagik Grigoryan, “Armbiotechnology” S&P Center of National Academy of Sciences of Armenia, Yerevan, Armenia
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Various factors; nutrition, hygiene, stress, infections, low immunity, menses, frequent sexual intercourses, pregnancy etc., destroyed vaginal microbial balance, which reflected in the number of lactobacilli decrease and increase the number of pathogenic microorganisms, as well as the pH-value. All this in turn causes vaginosis. The aim of this study is creation of symbiotic association of probiotic bacteria (SAPB) with high colonization/recolonization properties for rapid response to vaginal acute and chronic challenges. It is expected that LABs symbiotic association will be more beneficial for vaginal colonization/recoclonization, because the lactococci are growth well at elevate pH 9.0-5.0 and by reducing of vaginal pH promote the growth of lactobacilli, adapted to low pH 6.5-3.5. In this study used lactobacilli biocompatible with cocci possessing high antimicrobial activity from laboratory LABs collection, isolated earlier from 20-45 years old healthy Ar-menian woman volunteers (n=40). And it is the first time that lactococci were used as probiotic in vaginal coloniza-tion/recolonization practice. The symbiotic association of Lactobacillus plantarum (L. plantarum) GH 202 and Lactococcus lactis (L. lactis) GH 204 strains shows higher inhibitory activities against bacterial pathogens and Candida albicans (C. albicans). The SAPB growth rate is more intensive and biomass accumulation is higher, than the mono cultures. It is sustainable at different pH conditions of growth and during multiple subculturing imitating up and down changes of vaginal pH. The SAPB strains have high hydrophobicity evaluated by MATS test and high coaggregation properties essential for increasing their colonization potential, when they used in mixed culture.
LABs Association, Antimicrobial Activity, Adhesion, Coaggregation
To cite this article
Hrachya Garegin Hovhannisyan, Gohar Gagik Grigoryan, A New Sustainable Symbiotic Association of Lactic Acid Cocci and Bacilli for Colonization/Recolonization of Vagina and Prevention of Bacterial Vaginosis, American Journal of BioScience. Vol. 2, No. 3, 2014, pp. 84-88. doi: 10.11648/j.ajbio.20140203.11
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