Phycoremediation of Pb+2, Cd+2, Cu+2, and Cr+3 by Spirulina platensis (Gomont) Geitler
Phycoremediation is an application of algae for remediate environmental pollution by reducing or eliminating toxicity of such components in waste water. One of environmental problem in Indonesia is heavy metals pollution. Heavy metals are required by microalgae in a trace concentration, but in a high concentration heavy metals are toxic. Spirulina platensis is a microalgae belong to Cyanobacteria that has been used as a single cell protein, feed fish, and supplement. Many researchs on S. platensis had been done to optimize the product. Research on the use of S. platensis for remediation environmental pollution were also had been conducted. But, research on the use of local S. platensis to reduce the concentration of Pb2+, Cd2+, Cu2+ and Cr2+, had not yet well developed. Therefore, this research was conducted in order to find out the effect of 1, 3, and 5 mg/L concentrations of Pb2+, Cd2+, Cu2+ and Cr2+ on the S. platensis population growth and its accumulation. A laboratory experiment was developed with those different concentrations with 3 replications and control. The initial concentration of heavy metals were measured as well as the 7 and 15 days treatment to determine the bioaccumulation of heavy metals by S.platensis . Based on this research, the concentration of 1 mg/L heavy metals may induced the population growth of S. platensis, due to improvement enzymatic process, the concentration of 3 mg/L heavy metals inhibit population growth, and the concentration of 5 mg/L heavy metals was toxic for S. platensis. Since S. platensis was found high tolerance to Pb2+, Cd2+, Cu2+ and Cr2+at low concentration, it was recommended that for phycoremediation process using S. platensis more appropriate to be applied for low concentration contaminated water. It was suggested that S. platensis can be used as a metal absorbent and can be cultivated from wastewaters after phycoremediation process for other uses such as fertilizer, with the note that heavy metal contamination in waste water should not exceed 1 mg/L. S. platensis will be better if apply 15 days in contaminated wastewater to get the highest removal heavy metals.
Tri Retnaningsih Soeprobowati,
Phycoremediation of Pb+2, Cd+2, Cu+2, and Cr+3 by Spirulina platensis (Gomont) Geitler, American Journal of BioScience.
Vol. 2, No. 4,
2014, pp. 165-170.
T.R. Soeprobowati, H. Sugondo, I.B. Hendrarto, I. Sumantri and B. Toha, “Diatom and Ecological Changes of the River”, Seri Penelitian Fakultas Biologi, edisi khusus Prosiding Seminar Nasional Peranan Fungsi Ekologis dalam Pengelolaan Lingkungan. Universitas Satya Wacana, Salatiga, vol 4 no 2, 2001, 72-97
T.R. Soeprobowati, S. Hadisusanto and P. Gell, “The diatom stratigraphy of Rawapening Lake, Implying Eutrophication History”, Am. J. Env Sci vol 8 no 3, 2012, 334-344.
G. Banvalvi, “Cellular effects of heavy metals”, Springer, London, 201, 364.
J. Girard, “ Principles of environmental chemistry”, 2nd ed. Jones & Bartlett Publishers, LLC, 2010
C. Reynold, “Ecology of phytoplankton”, Cambrdige University Press. NY, 2006.
B. Volesky. “Biosorption and me”, Wat Res, vol 41, 2007, 4017-4029.
E.I. Olguin, “Phycoremediation: Key issues for cost-effective nutrient removal process”, Biotechnol. Adv. Vol 22, 2003, 81–91.
T.R. Soeprobowati and R. Hariyati, “Bioaccumulation of Pb, Cd, Cu, and Cr by Porphyridium cruentum (S.F. Gray) Nägeli”, Int J Mar Sci vol 3, no 27, 2013, 212-218.
K. Chojnacka, “Biosorption and Bioaccumulation in Practice”, Nova Science Publishers, Inc. New York, 2009, 149.
M.M.Emienour, S.M. Phang, and W.L. Chu, “Use of an algal consortium of five algae in the treatment of landfill leachate using the high-rate algal pond system” , J. Appl Phycol, vol 24, 2012, pp 953-963,
S. Bhatnagar, and R. Kumari, “Bioremediation: A Sustainable Tool for Environmental Management – A Review”, An Rev & Res in Bio vol 3 no 4, 2013, 974-993.
R. Hariyati, “ Pertumbuhan dan Biomassa Spirulina sp. dalam Skala Laboratoris”, BIOMA , vol 10 no 1, 2008, 19-22.
LM. Moreira, A.C. Ribeiro, F.A.Duarte, M.G.de Morais, and A.de.s. Soares, “Spirulina platensis biomass cultivated in Southern Brazil as a source of essential minerals and other nutrients”, Af. J. Food Scie vo 7, no 2, 2013, 451-455
A.C.A. Costa and F.P. Franca, “Cadmium Interaction with Microalgal Cells, Cyanobacterial Cells, and Seaweeds; Toxicology and Biotechnological Potential for Wastewater Treatment”, Mar Biotech vol 5, 2003, 149-156.
J.Z. Chen, X.C. Tao., J. Xu , T. Zhang , and Z.L. Liu., “Biosorption of lead, cadmium and mercury by immobilized Microcystis aeruginosa in a column”, Proc Biochem vol 40 no 12, 2005, 3675-3679.
K. Chojnacka, A. Chojnacka, and H. Gorecka, “Biosorption of Cr3+, Cd2+ and Cu2+ ions by blue–green algae Spirulina sp: kinetics, equilibrium and the mechanism of the process”, Chemosphere, vol 59, 2005, 75-84.
J. Miranda, G. Krishnakumar, and R. Gonsalves, “Cr6+ bioremediation efficiency of Oscillatoria laete-virens (Crouan and Crouan) Gomont and Oscillatoria trichoides Szafer: kinetics and equilibrium study”, Journal of Applied Phycology, vol 24, 2012, 1439-1454
J.A. Arnot, and F.A.P.C. Gobas, “A Review of Bioconcentration Factor (BCF) and Bioaccumulation Factor (BAF) Assessments for Organic Chemicals in Aquatic Organisms” Environ. Rev vol 14, 2006, 257-297.
A.I. Belokobylsky, E.I. Ginturi, N.E. Kuchava, E.I. Kirkesali, L. Mosulishvili, M.V. Frontasyeva, M.V. Pavlov, and N.G. Aksenova, “Accumulation of selenium and chromium in the growth dynamics of Spirulina platensis”. J. Radioanal. Nucl.Chem, 259 vol 1, 2004, 65-68.
T.R. Soeprobowati and R. Hariyati, “The Potential Used Of Microalgae For Heavy Metals Remediation”. Proceeding The 2nd International Seminar on New Paradigm and Innovation on natural Sciences and Its Application, Diponegoro University, Semarang Indonesia, 2012, 72-87.
T. Ivanciuc, O. Ivanciuc, and D.J. Klein, “Modeling The Bioconcentration Factors and Bioaccumulation Factors of Polychlorinated Biphenyls with Posetic Quantitative Super Structure/Activity Relationship (QSSAR)”, Mol Div, vol 10, 2006, 133-145.
T.R. Soeprobowati and R. Hariyati,” Phycoremediation of Pb, Cd, Cu, and Cr by Chaetoceros calcitrans (Paulsen) Takano”, Int’l Journal of Advances in Chemical Eng., & Biological Sciences (IJACEBS) Vol. 1, No 1, 2014, 37-40.
K.K.I.U. Arunakumara, Zhang, X., and Song, X., “ Bioaccumulation of Pb2+ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis”, J. Ocean Univ. Chin, vol 7, no 4, 2008, 397-403.
R.R. Siva Kiran, G.M. Madhu, S.V. Satyanarayana, and P. Bindiya, “Bioaccumulation of Cadmium in Blue Green Algae Spirulina (Arthrospira) indica”, J Bioremed Biodegrad vol 3, no 3, 2012, 141.
G. Disyawongs, “ Accumulation of Copper, Mercury and Lead in Spirulina platensis studied in Zarrouk’s medium”, The Journal of KMITNB, vol 12, no 4, 2002, 33.35.
M. Burzynski, and A. Zurek, “ Effects of copper and cadmium on photosynthesis in cucumber cotyledons”, Photosynthetica vol 45, 2007, 239–244.
L.R. Andrade, M. Farina, and G.M. Amado, “Effects of copper on Enteromorpha flexuosa Chlorophyta) in vitro”, Ecotoxicol. Environ. Safe vol 58, 2004, 117–125.
M. Baron, J.B. Arellano, and J.L. Gorge, “Copper and photosystem-II—a controversial relationship”, Physiol. Plant. 94, 1995, 174–180.
G.N. Babu, A.P. Sarma, and S.D.S. Murphy, “Effect of selected heavy metal ion on the photosynthetic electron transport and energy transfer in the thylakoid membrane of the Cyanobacterium, Spirulina platensis”, Af. J. Plant Scie. Vol 3 no 3, 2010, 118-121.
C. Solisio, A. Lodi, D. Soletto, A. Converti, “Cadmium biosorption on Spirulina platensis biomass”, Bioresource Tech vol 99, 2008, 5933-5937.
N. Roosens, N. Verbruggen, P. Meerts, P. Ximenez-Embun, J. Smith, “Natural variation in cadmium tolerance and its relationship to metal hyperaccumulation for seven populations of Thlaspi caerulescens from western Europe”, Plant Cell Env, vol 26, 2003, 1657-1672.