Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis

Aiman Al-Qtaitat; Said Al-Dalaen; Samir Mahgoub; Mohammad Al-Rawashdeh; Jean E. Aaron

doi:doi:10.11648/j.ajbio.20140206.15

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Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis

Aiman Al-Qtaitat, Said Al-Dalaen, Samir Mahgoub, Mohammad Al-Rawashdeh, Jean E. Aaron

Published in American Journal of BioScience (Volume 2, Issue 6)

Received: 13 November 2014 Accepted: 28 November 2014 Published: 2 December 2014

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Abstract

Osteoporosis particularly affects post-menopausal women for which the ovariectomized (OVX) rat is an established model and validation tool for agents of bone loss prevention. Objective: The potency of the natural substance propolis was investigated with regard to bone loss in ovariectomized animals. Methods: The complex chemical nature of propolis extract was confirmed by HPLC. Adult female albino rats (n=70; weight 150 – 200 gm), all ovariectomized at 24 weeks were randomly divided into controls (Group I, n=35; OVX/ H2O-supplement), for comparison with treatment (Group II, n=35; OVX/ propolis-supplement) administered as 400 mg of propolis/kg daily for 9 weeks. Blood biochemical analysis included serum calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), with serum osteocalcin (OC) determined by enzyme-linked immunosorbent assay (ELISA). Urine biochemical analysis measured Ca, P and creatinine (Cr). In addition, the corresponding densitometry of bone status comprised the bone mineral content (BMC) and density (BMD) at the proximal, distal and total femur by dual energy X-ray absorptiometry (DEXA; PIXImus), while the morphometry of the femoral shaft thickness was determined in longitudinal sections using an automated image analyzer. Results: A total of 27 compounds including flavonoids was identified in the propolis extract by HPLC. In comparison with OVX controls the skeleton was more substantial morphometrically in the animals receiving propolis, in terms of femoral shaft width (p<0.01) and BMD and BMC (p< 0.01). Simultaneously, the urine biochemical indices of Ca, P, Cr and Ca:Cr were significantly reduced (p<0.01) by propolis, while serum TRAP (an index of bone resorption) was also significantly lower (p<0.05), as were serum OC and ALP (indices of bone formation; p<0.05). Conclusion: Propolis is chemically complex and statistically bioactive in the oestrogen-deficient rodent, maintaining bone mass by reducing remodeling possibly by interaction with oestrogen receptors.

Published in	American Journal of BioScience (Volume 2, Issue 6)
DOI	10.11648/j.ajbio.20140206.15
Page(s)	217-221
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Ovariectomy-Induced Osteoporosis, Rat Femoral Densitometry, Biochemical Remodelling Indices, Propolis Constituents, Bone Loss Reduction, Bone Turnover Reduction

References

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APA Style

Aiman Al-Qtaitat, Said Al-Dalaen, Samir Mahgoub, Mohammad Al-Rawashdeh, Jean E. Aaron. (2014). Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. American Journal of BioScience, 2(6), 217-221. https://doi.org/10.11648/j.ajbio.20140206.15

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ACS Style

Aiman Al-Qtaitat; Said Al-Dalaen; Samir Mahgoub; Mohammad Al-Rawashdeh; Jean E. Aaron. Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. Am. J. BioScience 2014, 2(6), 217-221. doi: 10.11648/j.ajbio.20140206.15

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AMA Style

Aiman Al-Qtaitat, Said Al-Dalaen, Samir Mahgoub, Mohammad Al-Rawashdeh, Jean E. Aaron. Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. Am J BioScience. 2014;2(6):217-221. doi: 10.11648/j.ajbio.20140206.15

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@article{10.11648/j.ajbio.20140206.15,
  author = {Aiman Al-Qtaitat and Said Al-Dalaen and Samir Mahgoub and Mohammad Al-Rawashdeh and Jean E. Aaron},
  title = {Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {6},
  pages = {217-221},
  doi = {10.11648/j.ajbio.20140206.15},
  url = {https://doi.org/10.11648/j.ajbio.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140206.15},
  abstract = {Osteoporosis particularly affects post-menopausal women for which the ovariectomized (OVX) rat is an established model and validation tool for agents of bone loss prevention. Objective: The potency of the natural substance propolis was investigated with regard to bone loss in ovariectomized animals. Methods: The complex chemical nature of propolis extract was confirmed by HPLC. Adult female albino rats (n=70; weight 150 – 200 gm), all ovariectomized at 24 weeks were randomly divided into controls (Group I, n=35; OVX/ H2O-supplement), for comparison with treatment (Group II, n=35; OVX/ propolis-supplement) administered as 400 mg of propolis/kg daily for 9 weeks. Blood biochemical analysis included serum calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), with serum osteocalcin (OC) determined by enzyme-linked immunosorbent assay (ELISA). Urine biochemical analysis measured Ca, P and creatinine (Cr). In addition, the corresponding densitometry of bone status comprised the bone mineral content (BMC) and density (BMD) at the proximal, distal and total femur by dual energy X-ray absorptiometry (DEXA; PIXImus), while the morphometry of the femoral shaft thickness was determined in longitudinal sections using an automated image analyzer. Results: A total of 27 compounds including flavonoids was identified in the propolis extract by HPLC. In comparison with OVX controls the skeleton was more substantial morphometrically in the animals receiving propolis, in terms of femoral shaft width (p<0.01) and BMD and BMC (p< 0.01). Simultaneously, the urine biochemical indices of Ca, P, Cr and Ca:Cr were significantly reduced (p<0.01) by propolis, while serum TRAP (an index of bone resorption) was also significantly lower (p<0.05), as were serum OC and ALP (indices of bone formation; p<0.05). Conclusion: Propolis is chemically complex and statistically bioactive in the oestrogen-deficient rodent, maintaining bone mass by reducing remodeling possibly by interaction with oestrogen receptors.},
 year = {2014}
}

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TY  - JOUR
T1  - Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis
AU  - Aiman Al-Qtaitat
AU  - Said Al-Dalaen
AU  - Samir Mahgoub
AU  - Mohammad Al-Rawashdeh
AU  - Jean E. Aaron
Y1  - 2014/12/02
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140206.15
DO  - 10.11648/j.ajbio.20140206.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 217
EP  - 221
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140206.15
AB  - Osteoporosis particularly affects post-menopausal women for which the ovariectomized (OVX) rat is an established model and validation tool for agents of bone loss prevention. Objective: The potency of the natural substance propolis was investigated with regard to bone loss in ovariectomized animals. Methods: The complex chemical nature of propolis extract was confirmed by HPLC. Adult female albino rats (n=70; weight 150 – 200 gm), all ovariectomized at 24 weeks were randomly divided into controls (Group I, n=35; OVX/ H2O-supplement), for comparison with treatment (Group II, n=35; OVX/ propolis-supplement) administered as 400 mg of propolis/kg daily for 9 weeks. Blood biochemical analysis included serum calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), with serum osteocalcin (OC) determined by enzyme-linked immunosorbent assay (ELISA). Urine biochemical analysis measured Ca, P and creatinine (Cr). In addition, the corresponding densitometry of bone status comprised the bone mineral content (BMC) and density (BMD) at the proximal, distal and total femur by dual energy X-ray absorptiometry (DEXA; PIXImus), while the morphometry of the femoral shaft thickness was determined in longitudinal sections using an automated image analyzer. Results: A total of 27 compounds including flavonoids was identified in the propolis extract by HPLC. In comparison with OVX controls the skeleton was more substantial morphometrically in the animals receiving propolis, in terms of femoral shaft width (p<0.01) and BMD and BMC (p< 0.01). Simultaneously, the urine biochemical indices of Ca, P, Cr and Ca:Cr were significantly reduced (p<0.01) by propolis, while serum TRAP (an index of bone resorption) was also significantly lower (p<0.05), as were serum OC and ALP (indices of bone formation; p<0.05). Conclusion: Propolis is chemically complex and statistically bioactive in the oestrogen-deficient rodent, maintaining bone mass by reducing remodeling possibly by interaction with oestrogen receptors.
VL  - 2
IS  - 6
ER  -

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Author Information

Aiman Al-Qtaitat

Department of Anatomy and Histology, Faculty of Medicine, Mutah University, Karak, Jordan
Said Al-Dalaen

Department of Pharmacology, Faculty of Medicine, Mutah University, Karak, Jordan
Samir Mahgoub

Department of Biochemistry and Molecular Biology, Faculty of Medicine, Mutah University, Karak, Jordan
Mohammad Al-Rawashdeh

Department of Orthopaedic Surgery, Alkarak Governmental Hospital, Karak, Jordan
Jean E. Aaron

School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK

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Aiman Al-Qtaitat, Said Al-Dalaen, Samir Mahgoub, Mohammad Al-Rawashdeh, Jean E. Aaron. (2014). Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. American Journal of BioScience, 2(6), 217-221. https://doi.org/10.11648/j.ajbio.20140206.15

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ACS Style

Aiman Al-Qtaitat; Said Al-Dalaen; Samir Mahgoub; Mohammad Al-Rawashdeh; Jean E. Aaron. Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. Am. J. BioScience 2014, 2(6), 217-221. doi: 10.11648/j.ajbio.20140206.15

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AMA Style

Aiman Al-Qtaitat, Said Al-Dalaen, Samir Mahgoub, Mohammad Al-Rawashdeh, Jean E. Aaron. Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis. Am J BioScience. 2014;2(6):217-221. doi: 10.11648/j.ajbio.20140206.15

Copy | Download

@article{10.11648/j.ajbio.20140206.15,
  author = {Aiman Al-Qtaitat and Said Al-Dalaen and Samir Mahgoub and Mohammad Al-Rawashdeh and Jean E. Aaron},
  title = {Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {6},
  pages = {217-221},
  doi = {10.11648/j.ajbio.20140206.15},
  url = {https://doi.org/10.11648/j.ajbio.20140206.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140206.15},
  abstract = {Osteoporosis particularly affects post-menopausal women for which the ovariectomized (OVX) rat is an established model and validation tool for agents of bone loss prevention. Objective: The potency of the natural substance propolis was investigated with regard to bone loss in ovariectomized animals. Methods: The complex chemical nature of propolis extract was confirmed by HPLC. Adult female albino rats (n=70; weight 150 – 200 gm), all ovariectomized at 24 weeks were randomly divided into controls (Group I, n=35; OVX/ H2O-supplement), for comparison with treatment (Group II, n=35; OVX/ propolis-supplement) administered as 400 mg of propolis/kg daily for 9 weeks. Blood biochemical analysis included serum calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), with serum osteocalcin (OC) determined by enzyme-linked immunosorbent assay (ELISA). Urine biochemical analysis measured Ca, P and creatinine (Cr). In addition, the corresponding densitometry of bone status comprised the bone mineral content (BMC) and density (BMD) at the proximal, distal and total femur by dual energy X-ray absorptiometry (DEXA; PIXImus), while the morphometry of the femoral shaft thickness was determined in longitudinal sections using an automated image analyzer. Results: A total of 27 compounds including flavonoids was identified in the propolis extract by HPLC. In comparison with OVX controls the skeleton was more substantial morphometrically in the animals receiving propolis, in terms of femoral shaft width (p<0.01) and BMD and BMC (p< 0.01). Simultaneously, the urine biochemical indices of Ca, P, Cr and Ca:Cr were significantly reduced (p<0.01) by propolis, while serum TRAP (an index of bone resorption) was also significantly lower (p<0.05), as were serum OC and ALP (indices of bone formation; p<0.05). Conclusion: Propolis is chemically complex and statistically bioactive in the oestrogen-deficient rodent, maintaining bone mass by reducing remodeling possibly by interaction with oestrogen receptors.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Bioactive Propolis and Bone Loss Reduction in an Ovariectomized Rat Model of Hypogonadal Osteoporosis
AU  - Aiman Al-Qtaitat
AU  - Said Al-Dalaen
AU  - Samir Mahgoub
AU  - Mohammad Al-Rawashdeh
AU  - Jean E. Aaron
Y1  - 2014/12/02
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140206.15
DO  - 10.11648/j.ajbio.20140206.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 217
EP  - 221
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140206.15
AB  - Osteoporosis particularly affects post-menopausal women for which the ovariectomized (OVX) rat is an established model and validation tool for agents of bone loss prevention. Objective: The potency of the natural substance propolis was investigated with regard to bone loss in ovariectomized animals. Methods: The complex chemical nature of propolis extract was confirmed by HPLC. Adult female albino rats (n=70; weight 150 – 200 gm), all ovariectomized at 24 weeks were randomly divided into controls (Group I, n=35; OVX/ H2O-supplement), for comparison with treatment (Group II, n=35; OVX/ propolis-supplement) administered as 400 mg of propolis/kg daily for 9 weeks. Blood biochemical analysis included serum calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), with serum osteocalcin (OC) determined by enzyme-linked immunosorbent assay (ELISA). Urine biochemical analysis measured Ca, P and creatinine (Cr). In addition, the corresponding densitometry of bone status comprised the bone mineral content (BMC) and density (BMD) at the proximal, distal and total femur by dual energy X-ray absorptiometry (DEXA; PIXImus), while the morphometry of the femoral shaft thickness was determined in longitudinal sections using an automated image analyzer. Results: A total of 27 compounds including flavonoids was identified in the propolis extract by HPLC. In comparison with OVX controls the skeleton was more substantial morphometrically in the animals receiving propolis, in terms of femoral shaft width (p<0.01) and BMD and BMC (p< 0.01). Simultaneously, the urine biochemical indices of Ca, P, Cr and Ca:Cr were significantly reduced (p<0.01) by propolis, while serum TRAP (an index of bone resorption) was also significantly lower (p<0.05), as were serum OC and ALP (indices of bone formation; p<0.05). Conclusion: Propolis is chemically complex and statistically bioactive in the oestrogen-deficient rodent, maintaining bone mass by reducing remodeling possibly by interaction with oestrogen receptors.
VL  - 2
IS  - 6
ER  -

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