Diversity and Anti-microbial Activities of Actinomycetes Associated with Three Species of Lichens
American Journal of BioScience
Volume 3, Issue 5, September 2015, Pages: 171-177
Received: Jul. 6, 2015; Accepted: Jul. 14, 2015; Published: Jul. 28, 2015
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Authors
Yi Jiang, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China
Xinyu Wang, Key Lab for Plant Diversity & Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
Guiding Li, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China
Qinyuan Li, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China; Life Sciences Lab Center, School of Life Sciences, Yunnan University, Kunming, P. R. China
Chengbin Liu, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China; Institute of Microbial pharmaceuticals, College of Life and Health Science, Northeastern University, Shenyang, P. R. China
Xiu Chen, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China; Institute of Microbial pharmaceuticals, College of Life and Health Science, Northeastern University, Shenyang, P. R. China
Lisong Wang, Key Lab for Plant Diversity & Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
Yong Li, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China
Chenglin Jiang, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, P. R. China
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Abstract
In order to find new actinomycete resources for discovering new drug leads, the actinomycete associated with lichens was studied. Three species of lichen samples were collected from 3 locations of Yunnan. Actinomycetes in the samples were isolated with 11 media, and identified with 16S rRNA gene sequence procedures. Bacteria of two of the three species were sequenced by using 454 pyrosequencing. The results of both pure culture and 454 pyrosequencing were analyzed and compared. Anti-microbial activities of pure cultural strains were determined with agar diffusion methods. 17 genera of actinobacteria were isolated and identified from Lepraria yunnaniana, and 10 genera of them were exclusive. 12 genera were isolated and identified from Punctelia borreria, and 4 genera of them were exclusive. 11 genera were isolated and identified from Parmotrema austrosinense, and 5 genera were exclusive. Total 28 genera were isolated and identified from the three lichens. Streptomyces, Rhodococcus and Nocardia were distributed widely in the three species of lichens. The results from 454 pyrosequencing revealed total 567 taxa of bacteria were detected; the phylum actinobacteria of them had 107, and was 19%. The phylum Actinobacteria from Lepraria yunnaniana had 99, and from Punctelia borreri had 92. Taxonomic positions of 33 taxa belonging to the phylum Actinobacteria were not identified by using the method. These results showed that the diversity of actinomycetes associated with the three lichens was complex and different from each other. 20%, 19%, 12%, 17%, 9%, and 11 % actinomycete strains had anti-microbial activities against Bacillus subtilis subsp. Subtilis, Staphylococcus aureus subsp. Aureus, Escherichia coli, Pseudomonas aeruginosa, Mycobacterium tuberculosis and Candida albicans respectively. There are 30,000 species of lichens on the earth. Diversity of lichen-associated actinomycetes was very rich, and the anti-microbial activities were higher. Therefore lichen-associated actinomycetes are an important source for discovering new drug leads.
Keywords
Actinomycetes, Diversity, Antimicrobial Activity, Pyrosequencing, 16S rRNA, Lichen
To cite this article
Yi Jiang, Xinyu Wang, Guiding Li, Qinyuan Li, Chengbin Liu, Xiu Chen, Lisong Wang, Yong Li, Chenglin Jiang, Diversity and Anti-microbial Activities of Actinomycetes Associated with Three Species of Lichens, American Journal of BioScience. Vol. 3, No. 5, 2015, pp. 171-177. doi: 10.11648/j.ajbio.20150305.12
References
[1]
Alexander F. Auch, Mathias von Jan, Hans-Peter Klenk, Markus Göker Digita (2010) DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Standards in Genomic Sciences 2:117-134
[2]
An SY, Xiao T, Yokota A (2009) Leifsonia lichenia sp. nov., isolated from lichen in Japan. J Gen Appl Microbiol 55: 339–343
[3]
Bérdy J (2005) Bioactive Microbial Metabolites. A Personal View. J Antibiotics 58(1): 1–26
[4]
Bérdy J (2012) Thoughts and facts about antibiotics: Where we are now and where we are heading. J Antibiotics 65:385–395
[5]
Cui XL, Mao PH, Zeng M, Xu LH and Jiang CL 2001 Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51: 357- 363
[6]
Feuerer T, Hawksworth DL (2007) Biodiversity of lichens, including a world-wide analysis of checklist data based on Takhtajan’s floristic regions. Biodiversity and Conservation 16 (1): 85-98
[7]
Goodfellow M, Kämpfer P, Busse HJ, Trujillo ME, Suzuki K, Ludwig W, and Whitman WB (2012) Begey's Manual of Systematic Bacteriology. 2nd eds. Vol 5, The Actinobacteria, Part A, B. Springer, New York
[8]
González I, Ayuso-Sacido A, Anderson A, Genilloud O (2005) Actinomycetes isolated from lichens: evaluation of their diversity and detection of biosynthetic gene sequences. FEMS Microbiol Ecology 54(3):401-15
[9]
Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Fermentation Technology 65:501-509
[10]
Hideki Y, Haruna A, Youji N, Moriyuki H, Yuumi I, Misa O, Tomohiko T, Masayuki H (2011) Actinomycetospora iriomotensis sp. nov., a novel actinomycete isolated from a lichen sample. J Antibiotics 64:289-292
[11]
Jiang Y, Cao YR, Zhao LX, Wang Q, Jin RX, He WX, Xue QH (2010) Treatment of ultrasonic to soil sample for increase of the kind of rare actinomycetes. Acta Microbiol Sinica 50(8):1094-1097, 2010
[12]
Jiang Y, Cao YR, Zhao LX, Tang SK, Wang Y, Li WJ, Xu P, Lou K, Mao PH, Xu LH (2011) Large numbers of new bacterial taxa found by Yunnan Institute of Microbiology. Chinese Science Bulletin 56(8): 709-712
[13]
Kim SJ, Lee SS (2011) Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae. Int J Syst Evol Microbiol 61: 155-159
[14]
Kupchan SM, Kopperman HL (1975) l-Usnic acid: Tumor inhibitor isolated from lichens. Experientia 31(6): 625-631
[15]
Li B, Xie CH, Yokota A (2007) Nocardioides exalbidus sp. nov., a novel actinomycete isolated from lichen in Izu-Oshima Island, Japan. Actinomycetologica 21:22–26
[16]
Li H (2009) Co Variation analysis of human gut microbial structure and host global metabolism. Ph. D. thesis, Shanghai Jiao Tong University
[17]
Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemen LA, Berka J, Braverman AM, Chen YJ, Chen ZT, Dewell SB, Du L, Fierro JM, Gomes XV, Godwin BC, He W, Helgesen S, Ho CH, Irzyk GP, Jando SC, Lanza JR, Leamon JH, Lefkowitz SM, Lei M, Li J, Lohman KL, Lu H, Makhijani VB, McDade KE, Mckenna MP, Myers EW, Nickerson E, Nobile JR, Plant R, Puc BP, Ronan MT, Roth GT, Sarkis GJ, Simons JF, Simpson JW, Srinivasan M, Tartaro KR, Tomasz A, Vogt KA, Volkmer GA, Wang SH, Wang Y, Weiner MP, Yu PG, Berley RF, Rothberg JM (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437(7057): 376-380
[18]
Matsumoyo A, Bakir MA, Kudo T, Omura S, Takahashi Y (2008) Pseudosporangium ferrugineum gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 58: 1644-1652
[19]
Murray RGE, Schleifer KH (1994) Taxonomic Notes: A Proposal for Recording the Properties of Putative Taxa of Procaryotes. Internationajol Urnal of Systematibca Cteriologyj a n P. 174-176
[20]
Olga G, Ignacio G, Oscar S, Jesus M, José RT (2011) Current approaches to exploit actinomycetes as a source of novel natural products. Francisca Vicente J Industrial Microbiology & Biotechnology 38(3):375-389
[21]
Orsini M and Romano-Spica V 2001 A micrwave-based method for nucleic acid isolation from environmental samples. Lett Appl Microbiol 33:17- 20
[22]
Pankratov TA (2012) Acidobacteria in microbial communities of the bog and tundra lichens. Microbiology 81(1): 51-58
[23]
Santiago KAA, Sangvichien E, Boonpragob K, dela Cruz TEE (2013) Secondary metabolic profiling and antibacterial activities of different species of Usnea collected in Northern Philippines. Mycosphere 4(2):267–280
[24]
Sinem A, Kadir K (2013) The Investigation of Antibacterial Activities of Ethanol and Methanol Extracts of Flavoparmelia caperata (L.) Hale (Parmeliaceae) and Roccella phycopsis Ach. (Roccellaceae) Lichens Collected from Eastern. J App Pharmaceutical Science 3 (2):143-147
[25]
Sunil KKC, Klaus M (1999) Lichen Metabolites. 2. Antiproliferative and Cytotoxic Activity of Gyrophoric, Usnic, and Diffractaic Acid on Human Keratinocyte Growth. J Natural Products 62(6):821-823
[26]
Vertika S, Geeta PJ, Rawat MSM (2010) Lichens as a potential natural source of bioactive compounds: a review. Phytochemistry Reviews 9(2): 303-314
[27]
Wang LS (2012) Lichens in Yunnan of China. Shanghai Science and Technology Publishers, Shanghai
[28]
Wang LS, Qian ZG (2013) Pictorial handbook to medicinal lichens in Chin. Yunnan Provincial Science and Technology Publishers, Kunming
[29]
Wang TT, Cai GX, Qiu YP, Na F, Zhang MH, Pang XY, Jia W, Cai SJ, Zhao LP (2012) Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. The ISME journal 6(2): 320-329
[30]
Wei JC (1991) Overviews of Chinese lichens. International Academic Publishers, Beijing
[31]
Xu LH, Li WJ, LIU ZH, Jiang CL (2007) Actinomycete Systematic——principle, practice and Methods. Academic Press, Beijing
[32]
Xu LH, Lou K, Zhang H, Zhang LP, Xue QH, Xiong Z (2010) Microbial resources science 2nd edn. Academic Press, Beijing
[33]
Yassin AF, Hupfer HW (2006) Williamsia deligens sp. nov., isolated from human blood. I Int J Syst Evol Microbiol 56:193–197
[34]
Zhang HH, Chen L (2010) Phylogenetic analysis of 16S rRNA gene sequences reveals distal gut bacterial diversity in wild wolves (Canis lupus). Molecular Biology Reports 37: 4013-4022
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