Artificial Cultivation of Ganoderma lucidum (Reishi Medicinal Mushroom) Using Different Sawdusts as Substrates
American Journal of BioScience
Volume 3, Issue 5, September 2015, Pages: 178-182
Received: Jul. 12, 2015;
Accepted: Jul. 28, 2015;
Published: Aug. 5, 2015
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Subarna Roy, Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Miskat Ara Akhter Jahan, Plant Pathology Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhanmondi, Dhaka, Bangladesh
Kamal Kanta Das, Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Saurab Kishore Munshi, Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Rashed Noor, Department of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Ganoderma lucidum is a kind of medicinal mushroom possessing anti-tumor, anti-inflammatory, immune-modulatory, antioxidant and other biological traits which render it to be used as medicinal herbs to combat against variety of diseases. Present study was designed to implement a suitable method for artificial cultivation of G. lucidum in polypropylene bags with variety of cheap and readily available substrates. Sawdusts of five woods (Swietenia mahagoni, Dipterocarpus turbinatus, Tectona grandis, Gmelina arborea and Michelia champaca) were used as substrates and each was supplemented with calcium carbonate (CaCO3) and either rice or wheat bran for cultivation. T. grandis, G. arborea and M. champaca were not found to provoke the further extension of mycelial growth and hence the growth was stunted. On the contrary, S. mahagoni and D. turbinatus were noticed to impart comparatively good yield with biological efficiency. Wheat bran was found to be more efficient as supplement than rice bran. However, S. mahagoni supplemented with wheat bran provided the best yield of mushroom among the substrates which took 6 days, 33 days and 60 days for the mycelial growth, primordial formation and harvesting, consecutively with the subsequent yields of 235.2 g/kg and biological efficiency of 7.6%.
Miskat Ara Akhter Jahan,
Kamal Kanta Das,
Saurab Kishore Munshi,
Artificial Cultivation of Ganoderma lucidum (Reishi Medicinal Mushroom) Using Different Sawdusts as Substrates, American Journal of BioScience.
Vol. 3, No. 5,
2015, pp. 178-182.
F. C. Yang and C. B. Liau, “Effect of cultivatingconditions on the mycelial growth of Ganoderma lucidum in submerged flask cultures,” Bioprocess Engineering, vol.19, pp. 233-236, 1998.
R. Wagner, D. A. Mitchell, G. L. Sassaki, M. A. L. A. Amazonas and M. Berovic, “Current techniques for the cultivation of Ganoderma lucidum for the production of bimass, ganoderic acid and polysaccharides,” Food Technol Biotechnol, vol. 41, pp. 371-382, 2003.
M. Azizi, M. Tavana, M. Farsi and F. Oroojalian, “Yield performance of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (W.Curt.:Fr.) P. Karst. (higher basidiomycetes), using different waste materials as substrates,” Intl J Med Mushr, vol.14, pp. 521–527, 2012.
S. Wachtel-Galor, J. Yuen, J. A. Buswell and I. F. F. Benzie, Herbal Medicine: Biomolecular and Clinical Aspects, 2nd ed. Boca Raton: CRC Press, 2011.
E. I. Erkel, “The effect of different substrate mediums on yield of Ganoderma lucidum (Fr.) Karst,” J Food Agri Environ vol. 7, pp. 841-844, 2009.
Z. X. Ling, A. F. Chen and Z. B. Lin, “Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice” J Ethnopharmacol, vol. 111, pp. 219-226, 1997.
K. Deepalakshmi and S. Mirunalini, “Therapeutic properties and current medicinal usages of medicinal mushroom: Ganoderma lucidum,” Int J Pharm Pharm Sci, vol. 2, pp. 1922-1929, 2011.
A. Kamra and A. B. Bhatt, “First attempt of an organic cultivation of red Ganoderma lucidum under subtropical habitat and its economics,” Intl J Pharm Pharma Sci, vol. 5, pp. 94-98, 2013.
T. A. Ajith and K. K. Janardhanan, “Indian medicinal mushrooms as a source of antioxidant and antitumor agents,” J Clin Biochem Nutr, vol. 40, pp. 157-162, 2006.
C. Hsieh and F. Yang, “Reusing soy residue for the solid-state fermentation of Ganoderma lucidum,” Bioresour Technol, vol. 91, pp. 105-109, 2004.
S. T. Chang and J. A. Buswell, “Safety, quality control and regulational aspects relating to mushroom nutriceuticals,” Proceedings of 6th International Conference in Mushroom Biology and Mushroom Products. GAMU Gmbh, Krefeld, Germany, 2008.
S. P. Wasser, Reishi or ling zhi (Ganoderma lucidum). Encyclopedia of Dietary Supplements, 2005.
B. Boh, M. Berovic, J. Zhang and L. Zhi-Bin, “Ganoderma lucidum and its pharmaceutically active compounds,” Biotechnol Ann. Rev, vol. 13, pp. 265–301, 2007.
H. M. Chen, Reutilization of waste materials from a rice distillery for the cultivation of Ganoderma lucidum. Taiwan: MS thesis, Tunghai University, 1998.
M. X. Wang and X. L. Gao, “Study on substrates for Ganoderma lucidum Karst. and the key to high-yield cultivation management,” Edible Fungi China vol. 1, pp. 17–18, 1990.
H. Ji, Q. Wang, H. Wang, W. J. Chen, Z. H. Zhu, H. Hou and W. Zhang, “Preliminary research on Flammulina velutipes and Ganoderma lucidum cultivation using maize straw,” Edible Fungi China, vol. 20, pp. 11–12, 2011.
F. C. Yang, C. Hsieh and H. M. Chen, “Use of stillage grain from a rice-spirit distillery in the solid state fermentation of Ganoderma lucidum,” Process Biochem, vol. 39, pp. 21–26, 2003.
C. K. Tiwari, P. B. Meshram and A. K. Patra, “Artificial cultivation of Ganoderma lucidum,” Indian Forester, vol. 130, pp. 1057–1059, 2004.
A. J. Kakon, M. B. K. Choudhury and S. Saha, “Mushroom is an ideal food supplement,”.J Dhaka National Med Coll Hos, vol. 18, pp. 58-62, 2012.
M. N. Uddin, S. Yesmin, M. A. Khan, M. Tania, M. Moonmoon and S. Ahmed, “Production of oyster mushrooms in different seasonal conditions of Bangladesh,” J Sci Res, vol. 3, pp. 161-167, 2011.
A. W. Chen, Mushroom Growers Handbook. Seul, Korea: MushWorld-Heineart Inc., 2012.
P. Stamets, Growing Gourmet and Medicinal Mushrooms. Berkeley: Ten Speed Press, 2010.
S. Singh, N. S. K. Harsh and P. K. Gupta, “A novel method of economical cultivation of medicinally important mushroom, Ganoderma lucidum,” Intl J Pharm Sci Res, vol. 5, pp. 2033-2037, 2014.
D. J. Royse, “Effect of spawn run time and substrate nutrition on yield and size of the shiitake mushroom,” Mycologia, vol. 77, pp. 756–62, 1985.
G. M. Cragg and D. J. Newman, “Natural products: a continuing source of novel drug leads,” Biochim Biophys Acta, vol. 1830, pp. 3670-3695, 2013.
R. Noor, N. Huda, F. Rahman, T. Bashar and S. K. Munshi, “Microbial contamination in herbal medicines available in Bangladesh,” Bang Med Res Coun Bull, vol. 39, pp. 124-129, 2013.
T. Ahmed, N. J. Urmi, M. S. Munna, K. K. Das, M. Acharjee, M. M. Rahman and R. Noor, “Assessment of microbiological proliferation and in vitro demonstration of the antimicrobial activity of the commonly available salad vegetables within Dhaka metropolis, Bangladesh,” Am J Agri Forestr, vol. 2, pp. 55-60, 2014.
M. Sharmin, I. T. Nur, M. Acharjee, S. K. Munshi and R. Noor, “Microbiological profiling and the demonstration of in vitro anti-bacterial traits of the major oral herbal medicines used in Dhaka Metropolis,” SpringerPlus, vol. 3, pp. 739, 2014.
M. Sharmin, K. K. Das and M. Acharjee, “Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers,” Stamford J Microbiol, vol. 4, pp. 19-23, 2014.
S. Quaiyum, N. I. Tanu, M. Sharmin, L. Paul, S. Munna, K. K. Das, M. Acharjee and R. Noor, “Microbiological contamination and anti-bacterial traits of common oral herbal medicinal products within Dhaka metropolis,” Europian Journal of Medicinal Plant, vol. 4, pp. 872-881, 2014.
S. Somasundaram and K. Manivannan, “An overview of fluoroquinolones,” Annual Rev Res Biol, vol. 3, pp. 296-313, 2004.
World Health Organization, WHO Traditional Medicine Strategy: 2014-2023. Geneva, Switzerland: WHO Press, 2014.
S. Dutta, M. R. Hassan, F. Rahman, M. F. A. Jilani and R. Noor, “Study of antimicrobial susceptibility of clinically significant microorganims isolated from selected areas of Dhaka, Bangladesh,” Bang J Med Sci, vol. 12, pp. 34-42, 2013.
C. Veeresham, “Natural products derived from plants as a source of drugs,” J Adv Pharm Technol Res, vol. 3, pp. 200-201, 2012.
R. M. Gyasi, M. M. Charlotte, O. W. A. Prince and A. Seth, “Public perceptions of the role of traditional medicine in the health care delivery system in Ghana,” Global J Health Sci, vol. 3, pp. 40-49, 2011.
K. Kraft, “Complementary/alternative medicine in the context of prevention of disease and maintenance of health,” Prev Med, vol. 49, pp. 88-92, 2009.
A. A. Izzo and E. Ernst, “Interactions between herbal medicines and prescribed drugs: an updated systematic review,” Drugs vol. 69, pp. 1777-1798, 2009.
K. Malarvizhi, K. Murugesan and P. T. Kalaichelvan, “Xylamase production by Ganoderma lucidum on liquid and solid state culture,” Ind J Expl Biol, vol. 41, pp. 620-626, 2003.