Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats
American Journal of BioScience
Volume 3, Issue 5, September 2015, Pages: 183-189
Received: Jun. 19, 2015; Accepted: Jun. 29, 2015; Published: Aug. 19, 2015
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Authors
Charles Obiora Nwonuma, Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Emenike Onyebum Irokanulo, Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Owa Olugbenga Stephen, Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
Ucheabba Destinee Esther, Biochemistry Unit, Department of Biological Sciences, College of Science and Engineering, Landmark University, Omuaran Kwara State, Nigeria
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Abstract
Potassium bromate, a common ingredient in bakery industry, serves as leaven in flour dough. However previous studies have demonstrated that potassium bromate is toxic. This study evaluated the effect of eggshell powder solution (ESPS) on the testicular indices in Wistar rats administered with potassium bromate. Twenty Wistar rats weighing between 200-250 g were randomly grouped into 5: A, B, C, D and E; with 4 rats per group. Group A served as the positive control and was administered with 1ml of 10 mg/kg body weight of potassium bromate solution Group B served as the negative control which was administered with 1ml of distilled water. Groups C, D and E were administered with 1ml each of 200, 300 and 400 mg/kg body weight of ESPS respectively reconstituted in distilled water in addition to 1ml of 10 mg/kg body weight of potassium bromate. The treatment was carried out successively for 28 days before the animals were sacrificed 24 hours after the last day of treatment and their testes, harvested. Biochemical tests to evaluate the testicular function using UV/VIS spectrometer and histological tests on the testicular homogenate and tissues respectively were carried out. The result showed significant decrease (P<0.05) in the total protein, the superoxide dismutase activity (SOD) and malondialdhyde (MDA). Contrary, there was a significant increase (P<0.05) in the Reduced glutathione (GSH) and testicular glycogen of the test rats. The histopathology result showed normal germ cells at lower doses of the ESPS. This experiment showed marked reduction in the lipid peroxidation in rats’ testes given ESPS at a lower dose
Keywords
Testes, Brown Eggshell, Potassium Bromate, Histopathology
To cite this article
Charles Obiora Nwonuma, Emenike Onyebum Irokanulo, Owa Olugbenga Stephen, Ucheabba Destinee Esther, Protective Potentials of Brown Chicken Eggshell against Potassium Bromate Effect on Testicular Functional Indices in Wistar Rats, American Journal of BioScience. Vol. 3, No. 5, 2015, pp. 183-189. doi: 10.11648/j.ajbio.20150305.14
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