Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women
American Journal of BioScience
Volume 3, Issue 5, September 2015, Pages: 190-196
Received: Aug. 12, 2015; Accepted: Aug. 17, 2015; Published: Aug. 29, 2015
Views 3227      Downloads 73
Rebelo Ana Cristina Silva, Department of Morphology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Brazil
Melo Aryanne Batista Soares, Center for Neuroscience and Cardiovascular Physiology Research, Department of Physiological Sciences, Biological Sciences Institute, Federal University of Goiás, Goiânia, Brazil
Salviati Mariana Rodrigues, Humana Performance Research Group - College of Health Sciences (FACIS), Methodist University of Piracicaba (UNIMEP), Piracicaba, Brazil
Verlengia Rozangela, Humana Performance Research Group - College of Health Sciences (FACIS), Methodist University of Piracicaba (UNIMEP), Piracicaba, Brazil
Vale Arthur Ferreira Do, Department of Morphology, Biological Sciences Institute, Federal University of Goiás, Goiânia, Brazil
Nogueira Yanley Lucio, Faculty of Medicine, Federal University of Goiás, Goiânia, Brazil
Tamburús Nayara Yamada, Department of Physiotherapy, Federal University of Sao Carlos, Sao Carlos, Brazil
Kunz Vandeni Clarice, Adventist University of Sao Paulo, Engenheiro Coelho, Sao Paulo, Brazil
Silva Ester Da, Department of Physiotherapy, Federal University of Sao Carlos, Sao Carlos, Brazil
Article Tools
Follow on us
Background: Studies have shown an interaction between the autonomic heart rate (HR) modulation and genetic polymorphisms. Nevertheless, it is yet unclear how a particular genetic polymorphism may determine a pattern of autonomic HR control, especially regarding the role of physical exercise. The objective was to investigate the influence of of Beta-2-Adrenergic (β2-AR) Gln27Glu polymorphisms and exercise on HRV of young Brazilian women. Methods: The group was selected as Sedentary Group (SG; n = 88) and physically Active Group (AG; n = 99). The β2-AR polymorphisms were analyzed using PCR-RFLP. The amplicons were subjected to electrophoresis on a 10% acrylamide gel and stained with ethidium bromide. The HR was registered in real time for 15 minutes, in the supine-position. The heart rate variability (HRV) was analysed using Shannon’s entropy (SE), conditional entropy (complexity index [CInd] and normalized CInd [NCI]), and symbolic analysis (0V%, 1V%, 2LV%, and 2ULV%). Statistical analysis: Analysis of variance (ANOVA) with Fisher's Least. Results: AG presented higher values for SE and 2ULV%, and lower values for 0V% when compared to SG (p<0.05). There was no significant difference of any index among genotypes both from the complexity and symbolic analysis. The interaction between genotype and exercise did not significantly affect HRV. Conclusion: These results do not support the concept that the β2-AR Gln27Glu polymorphisms affects the HRV indexes in physically active or sedentary women.
β2-Adrenergic Polymorphism, Heart Rate Variability, Non-Linear Analysis, Women, Exercise
To cite this article
Rebelo Ana Cristina Silva, Melo Aryanne Batista Soares, Salviati Mariana Rodrigues, Verlengia Rozangela, Vale Arthur Ferreira Do, Nogueira Yanley Lucio, Tamburús Nayara Yamada, Kunz Vandeni Clarice, Silva Ester Da, Influence of Beta-2-Adrenergic Receptor Gln27Glu Polymorphism on the Autonomic Heart Rate Modulation in Young Sedentary and Physically Active Women, American Journal of BioScience. Vol. 3, No. 5, 2015, pp. 190-196. doi: 10.11648/j.ajbio.20150305.15
Gribble, M. O.; Cheng, A.; Berger, R. D.; Rosman, L.; Guallar, E. Mercury exposure and heart rate variability: A systematic review. Current environmental health reports 2015, 2, 304-314.
Lakusic, N.; Mahovic, D.; Kruzliak, P. Changes in heart rate variability after coronary artery bypass grafting and clinical importance of these findings. 2015, 2015, 680515.
Montano, N.; Porta, A.; Cogliati, C.; Costantino, G.; Tobaldini, E.; Casali, K. R.; Iellamo, F. Heart rate variability explored in the frequency domain: A tool to investigate the link between heart and behavior. Neuroscience and biobehavioral reviews 2009, 33, 71-80.
Thayer, J. F.; Yamamoto, S. S.; Brosschot, J. F. The relationship of autonomic imbalance, heart rate variability and cardiovascular disease risk factors. International journal of cardiology 2010, 141, 122-131.
Yperzeele, L.; van Hooff, R.J.; Nagels, G.; De Smedt, A.; De Keyser, J.; Brouns, R. Heart rate variability and baroreceptor sensitivity in acute stroke: A systematic review. International journal of stroke : official journal of the International Stroke Society 2015, 10, 796-800.
Su, S.; Lampert, R.; Zhao, J.; Bremner, J. D.; Miller, A.; Snieder, H.; Lee, F.; Khan, D.; Goldberg, J.; Vaccarino, V. Pleiotropy of c-reactive protein gene polymorphisms with c-reactive protein levels and heart rate variability in healthy male twins. The American journal of cardiology 2009, 104, 1748-1754.
Yang, A. C.; Tsai, S.J.; Hong, C. J.; Wang, C.; Chen, T. J.; Liou, Y. J.; Peng, C. K. Clustering heart rate dynamics is associated with beta-adrenergic receptor polymorphisms: Analysis by information-based similarity index. PloS one 2011, 6, e19232.
Sarpeshkar, V.; Bentley, D. J. Adrenergic-beta(2) receptor polymorphism and athletic performance. Journal of human genetics 2010, 55, 479-485.
Wagoner, L. E.; Craft, L. L.; Singh, B.; Suresh, D. P.; Zengel, P. W.; McGuire, N.; Abraham, W. T.; Chenier, T. C.; Dorn, G. W., 2nd; Liggett, S. B. Polymorphisms of the beta(2)-adrenergic receptor determine exercise capacity in patients with heart failure. Circulation research 2000, 86, 834-840.
Pereira, A.C.; Floriano, M.S.; Mota, G.F.; Cunha, R.S.; Herkenhoff, F.L.; Mill, J.G.; Krieger, J.E. Beta2 adrenoceptor functional gene variants, obesity, and blood pressure level interactions in the general population. Hypertension 2003, 42, 685-692.
Villares, S. M.; Mancini, M. C.; Gomez, S.; Charf, A. M.; Frazzatto, E.; Halpern, A. Associação entre polimorfismo gln27glu do receptor b2-adrenérgico e hipertensão arterial sistêmica em obesos mórbidos. Arquivos Brasileiros de Endocrinologia & Metabologia 2000, 44, 72-80.
Dorn, G. W., 2nd. Adrenergic signaling polymorphisms and their impact on cardiovascular disease. Physiological reviews 2010, 90, 1013-1062.
Nakashima, H.; Omae, K.; Nomiyama, T.; Yamano, Y.; Takebayashi, T.; Sakurai, Y. Beta-3-adrenergic receptor trp64arg polymorphism: Does it modulate the relationship between exercise and percentage of body fat in young adult japanese males? Environmental health and preventive medicine 2013, 18, 323-329.
Masuo, K.; Lambert, G. W. Relationships of adrenoceptor polymorphisms with obesity. Journal of obesity 2011, 2011, 609485.
Matsunaga, T.; Yasuda, K.; Adachi, T.; Gu, N.; Yamamura, T.; Moritani, T.; Tsujimoto, G.; Tsuda, K. Association of beta-adrenoceptor polymorphisms with cardiac autonomic modulation in japanese males. American heart journal 2007, 154, 759-766.
Atala, M. M.; Goulart, A.; Guerra, G. M.; Mostarda, C.; Rodrigues, B.; Mello, P. R.; Casarine, D.E.; Irigoyen, M. C.; Pereira, A. C.; Consolim-Colombo, F.M. Arg16gly and gln27glu beta2 adrenergic polymorphisms influence cardiac autonomic modulation and baroreflex sensitivity in healthy young brazilians. American journal of translational research 2015, 7, 153-161.
Bouchard, C. Genomic predictors of trainability. Experimental physiology 2012, 97, 347-352.
Macho-Azcarate, T.; Marti, A.; Calabuig, J.; Martinez, J.A. Basal fat oxidation and after a peak oxygen consumption test in obese women with a beta2 adrenoceptor gene polymorphism. The Journal of nutritional biochemistry 2003, 14, 275-279.
Castellano, M.; Rossi, F.; Giacche, M.; Perani, C.; Rivadossi, F.; Muiesan, M.L.; Salvetti, M.; Beschi, M.; Rizzoni, D.; Agabiti-Rosei, E. Beta(2)-adrenergic receptor gene polymorphism, age, and cardiovascular phenotypes. Hypertension 2003, 41, 361-367.
Porta, A.; Tobaldini, E.; Guzzetti, S.; Furlan, R.; Montano, N.; Gnecchi-Ruscone, T. Assessment of cardiac autonomic modulation during graded head-up tilt by symbolic analysis of heart rate variability. American journal of physiology. Heart and circulatory physiology 2007, 293, H702-708.
Heart rate variability. Standards of measurement, physiological interpretation, and clinical use. Task force of the european society of cardiology and the north american society of pacing and electrophysiology. European heart journal 1996, 17, 354-381.
Porta, A.; Guzzetti, S.; Montano, N.; Furlan, R.; Pagani, M.; Malliani, A.; Cerutti, S. Entropy, entropy rate, and pattern classification as tools to typify complexity in short heart period variability series. IEEE transactions on bio-medical engineering 2001, 48, 1282-1291.
Porta, A.; Faes, L.; Mase, M.; D'Addio, G.; Pinna, G. D.; Maestri, R.; Montano, N.; Furlan, R.; Guzzetti, S.; Nollo, G., et al. An integrated approach based on uniform quantization for the evaluation of complexity of short-term heart period variability: Application to 24 h holter recordings in healthy and heart failure humans. Chaos (Woodbury, N.Y.) 2007, 17, 015117.
Porta, A.; Baselli, G.; Liberati, D.; Montano, N.; Cogliati, C.; Gnecchi-Ruscone, T.; Malliani, A.; Cerutti, S. Measuring regularity by means of a corrected conditional entropy in sympathetic outflow. Biological cybernetics 1998, 78, 71-78.
Salazar, L.A.; Hirata, M.H.; Cavalli, S.A.; Machado, M.O.; Hirata, R.D. Optimized procedure for DNA isolation from fresh and cryopreserved clotted human blood useful in clinical molecular testing. Clinical chemistry 1998, 44, 1748-1750.
Rebelo, A.C.; Verlengia, R.; Kunz, V.; Tamburus, N.; Cerda, A.; Hirata, R.; Hirata, M.; Silva, E. Lack association of estrogen receptor alpha gene polymorphisms with cardiorespiratory and metabolic variables in young women. International Journal of Molecular Sciences 2012, 13, 13691-13703.
Dias, R.G.; Pereira, A.d.C.; Negrão, C.E.; Krieger, J.E. Polimorfismos genéticos determinantes da performance física em atletas de elite. Revista Brasileira de Medicina do Esporte 2007, 13, 209-216.
Wolfarth, B.; Rankinen, T.; Muhlbauer, S.; Scherr, J.; Boulay, M.R.; Perusse, L.; Rauramaa, R.; Bouchard, C. Association between a beta2-adrenergic receptor polymorphism and elite endurance performance. Metabolism: clinical and experimental 2007, 56, 1649-1651.
Meirhaeghe, A.; Helbecque, N.; Cottel, D.; Amouyel, P. Beta2-adrenoceptor gene polymorphism, body weight, and physical activity. Lancet 1999, 353, 896.
Rebelo, A.C.; Tamburus, N.; Salviati, M.; Celante, V.; Takahashi, A.; de Sa, M.F.; Catai, A.; Silva, E. Influence of third-generation oral contraceptives on the complexity analysis and symbolic dynamics of heart rate variability. The European journal of contraception & reproductive health care : the official journal of the European Society of Contraception 2011, 16, 289-297.
Marães, V.R.F.S. Frequência cardíaca e sua variabilidade: Análises e aplicações. Revista Andaluza de Medicina del Deporte 2010, 03, 33-42.
Clemente-Suarez, V.J.; Fernandes, R.J.; Arroyo-Toledo, J.J.; Figueiredo, P.; Gonzalez-Rave, J.M.; Vilas-Boas, J.P. Autonomic adaptation after traditional and reverse swimming training periodizations. Acta physiologica Hungarica 2015, 102, 105-113.
Rezende Barbosa, M.P.; Netto Junior, J.; Cassemiro, B.M.; de Souza, N.M.; Bernardo, A.F.; da Silva, A.K.; Pastre, C.M.; Vanderlei, L.C. Impact of functional training on cardiac autonomic modulation, cardiopulmonary parameters and quality of life in healthy women. Clinical physiology and functional imaging 2015.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186