Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes
American Journal of BioScience
Volume 3, Issue 6, November 2015, Pages: 249-255
Received: Dec. 25, 2014; Accepted: Jan. 5, 2015; Published: Nov. 13, 2015
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Author
Narinder Kumar Sharma, Radiation Biology and Health Sciences Division, Bio-Sciences Group, Bhabha Atomic Research Centre, Mumbai, India
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Abstract
Histamine H2 receptor antagonist are used in the clinical treatment of peptic ulcer. In vitro metaphase analysis and micronucleus assay were used to test the effect of Famotidine & Ranitidine on 60Cobalt gamma-ray induced clastogenic effects. Heparinised whole blood was obtained from 6 healthy volunteers and was gamma irradiated with 3Gy. Lymphocyte cultures were initiated and aqueous solution of Famotidine (150µg/ml) & Ranitidine (500µg/ml) was added at 0h and 24h. Cultures were harvested & processed at 48h & 72h for chromosome aberrations and micronucleus analysis respectively. At 0h & 24h after 3Gy gamma irradiation, cultures treated with Famotidine & Ranitidine independently showed significant decrease (p < 0.0001) in the frequency of chromosome aberration. At 0h & 24h Famotidine induced 60.91% & 56.42% inhibition in dicentrics & 59.39% & 56.21% inhibition was observed in total aberrations where as Ranitidine induce 52.11% & 43.54% inhibition in dicentrics and 53.06% & 46.66% inhibition in total aberrations at 0h & 24h. Significant decrease in the frequency of micronuclei was observed with Famotidine treatment after 3Gy of gamma irradiation, which induced inhibition of 48.83% (p < 0.0001) at 0hr & 5.02% (p < 0.016) at 24h. However, Ranitidine induced significant decrease (p < 0.0001) in frequency of micronuclei of 28.85% at 0h where as a decrease in frequency was observed of 2.88% at 24h although not significant when compared with 3Gy gamma irradiation alone. In conclusion radio protective effects of Histamine H2 receptor antagonists Famotidine and Ranitidine was observed on exposure togamma-ray.
Keywords
Ionizing Radiation, Radioprotection, Chromosomal Aberration, Cytochalasin-B Blocked Micronuclei, Histamine H2 Receptor Antagonist, Famotidine, Ranitidine, Human Lymphocytes
To cite this article
Narinder Kumar Sharma, Radioprotective Effects of Histamine H2 Receptor Antagonists Famotidine and Ranitidine on Gamma-Ray Induced Chromosome Aberration and Micronuclei in vitro in Human Lymphocytes, American Journal of BioScience. Vol. 3, No. 6, 2015, pp. 249-255. doi: 10.11648/j.ajbio.20150306.17
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