Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia
American Journal of BioScience
Volume 4, Issue 2, March 2016, Pages: 9-15
Received: Jun. 11, 2015; Accepted: Jun. 19, 2015; Published: Mar. 19, 2016
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Fekadu Alemu, Department of Biology, Dilla University, College of Natural and Computational Sciences, Dilla, Ethiopia
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The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.
Faba Bean, Indole Acetic Acid, Phytohormone, Plant Growth Promoting Rhizobacteria, Pseudomonas fluorescens
To cite this article
Fekadu Alemu, Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia, American Journal of BioScience. Vol. 4, No. 2, 2016, pp. 9-15. doi: 10.11648/j.ajbio.20160402.11
Ahemad, M. and Khan, M. S. (2010b). Ameliorative effects of Mesorhizobium sp.MRC4 on chickpea yield and yield components under different doses of herbicide stress. Pestc. Biochem. Physiol., 98: 183-190.
Barazani, O. and Friedman, J. (1999). Is IAA the major root growth factor secreted from plant growth mediated bacteria? J. Chem. Ecol., 25: 2397-2407.
Barker, S. J. and Tagu, D. (2000). The roles of auxins and cytokinins in mycorrhizal symbiosis. J. Plant Growth Regul., 19: 144-154.
Benizri, E., Courtade, A., Picard, C. and Guckert, A. (1998). Role of maize root exudates in the production of auxins by Pseudomonas fluorescens M.3.1: Short communication. Soil Biol. Biochem., 30: 1481-1484.
Bloemberg, G. V. and Lugtenberg, B. J. (2001). Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr. Opinion Plant Biol., 4: 343–350.
Bric, J. M., Bostock, R. M. and Silverstone, S. E. (1991). Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Environ. Microbiol., 57: 535-538.
Davies, P. (1995). The plant hormone concept: concentration, sensitivity, and transport. In: (Davies, P., ed) Hormones: Physiology, Biochemistry, and Molecular Biology, Plant. Kluwer Academic Publishers, Dordrecht, pp.13–18.
Davies, P. J. (2010). The Hormones: Their Nature, Occurrence and Functions. Kluwer Academic, New York.
De Freitas, J. R. and Germide, J. J. (1990). Plant growth promoting rhizobacteria for winter wheat. Can. J. Microbiol., 36: 265-272.
Dubeikovsky, A. N., Mordukhova, E. A., Kochetkov, V. V., Polikarpova, F. Y. and Boronin, A. M. (1993). Growth promotion of blackcurrant softwood cuttings by recombinant strain Pseudomonas fluorescens BSP53a synthesizing an increased an increased amount of indole-3-acetic acid. Soil Biol. Biochem., 25: 1277-1281.
Frankenberger, J. W. T. and Arshad, M. (1991). Microbial production of plant growth regulating substances in soil. In: (Keel, C., Koller, B. and Defago, G., eds) Plant Growth-Promoting Rhizobacteria, Progress and Prospects. The Second International Workshop on PGPR, Interlaken, pp.162-171.
Glick, B. R. (1995). The enhancement of plant growth by free living bacteria. Can. J. Microbiol., 41: 109-117.
Gray, E. J. and Smith, D. L. (2005). Intracellular and extracellular PGPR: commonalities and distinctions in the plant-bacterium signaling processes. Soil Biol. Biochem., 37: 395-412.
Gupta, A., Meyer, J. M. and Goel, R. (2002). Development of heavy metal resistant mutants of phosphate solubilizing Pseudomonas sp. NBR14014 and their characterization. Curr. Microbiol., 45: 323-327.
Hirsch, A. M. and Fang, Y. (1994). Plant hormones and nodulation: What’s the connection. Plant Mol. Biol., 26: 5-9.
Kiely, P. D., Haynes, J. M., Higgins, C. H., Franks, A., Mark, G. L., Morrissey, J. P. and O’Gara, F. (2006). Exploiting new systems-based strategies to elucidate plant-bacterial interactions in the rhizosphere. Microbiol. Ecol., 51: 257–266.
King, E. O., Ward, M. K. and Raney, D. E. (1954). Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med., 44: 301-307.
Lalande, R., Bissonnette, N. Coutlée, D. and Antoun, H. (1989). Identification of rhizobacteria from maize and determination of their plant-growth promoting potential. Plant Soil, 115: 7-11.
Lifshitz, R., Klopper, J. W., Kozlowshi, M., Simonson, C., Carlson, J., Tipping, M. and Zalesha, I. (1987). Growth promation of Canola (rapeseed) seedling by a strain of Pseudomonas putida under gnotobiotic conditions. Can. J. Microbiol., 33: 390-395.
Loper, J. E. and Schroth, M. N. (1986). Influence of bacterial source of indole-3-acetic acid of root elongation of sugar beet. Phytopathol., 76: 386-389.
Malamy, J. E. and Benfry, P.N. (1997). Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development, 124: 33-44.
Mirza, M. S., Ahmad, W., Latif, F., Haurat, J., Bally, R., Normand, P. and Malik, K. A. (2001). Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro. Plant Soil, 237: 47-54.
Moeinzadeh, A., Sharif-Zadeh, F., Ahmadzadeh, M. and Heidari, T. F. (2010). Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Aust. J. Crop Sci., 4: 564-570.
Nagarajkumar, M., Bhaskaran, R. and Velazhahan, R. (2004). Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen. Microbiol. Res., 159: 73-81.
Nasr, N., Hovhannisyan, H. G., Pourkazemi, M., Azizzadeh, L.(2014). Molecular Characterization and Phylogenetic Analysis of Growth Hormone cDNA Sequence from the Acipenser Persicus, American Journal of BioScience. 2 (2): 79-83. doi: 10.11648/j.ajbio.20140202.20.
Oberha’nsli, T., De’fago, G. and Haas, D., (1991). Indole-3-acetic acid (IAA) synthesis in the biocontrol strain CHAO of Pseudomonas fluorescens: role of tryptophan side chain oxidase. J. Gen. Microbiol., 137: 2273-2279.
Okon, Y. and Kapulnik, Y. (1986). Development and function of Azospirillum inoculated roots. Plant and Soil, 90: 3-16.
Okon, Y. and vanderleyden, J. (1997). Root-associated Azospirillum species can stimulates plants. Am. Soc. Microbiol., 63: 366-370.
Pattern, C. L. and Glick, B. R. (2002). Bacterial biosynthesis of indole-3-acetic acid. Can. J. Microbiol., 42: 207-220.
Ramyasmruthi, S., Pallavi, O., Pallavi, S., Tilak, K. and Srividya, S. (2012). Chitinolytic and secondary metabolite producing Pseudomonas fluorescens isolated from Solanaceae rhizosphere effective against broad spectrum fungal phytopathogens. Asian J. Plant Sci. Res., 2: 16-24.
Rangajaran, S., Saleena, L.M., Vasudevan, P. and Nair, S. (2003). Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Plant Soil, 251: 73–82.
Salisbury, F. B. (1994). The Role of Plant Hormones in Plant Environment Interactions. Marcel Dekker, New York.
Spaepen, S., Vanderleyden, J. and Remans, R. (2007). Indole-3-acetic acid in microbial and microorganism-plant signaling. Microbiol. Rev., 31: 425-448.
Suresh, A., Pallavi, P., Srinivas, P., Kumar, V.P., Chandra, S.J. and Reddy, S.R. (2010). Plant growth promoting activities of fluorescent Pseudomonads associated with some crop plants. Afr. J. Microbiol. Res., 4: 1491-1494.
Suzuki, S., HE, Y. and Oyaizu, H. (2003). Indole-3-acetic acid production in Pseudomonas fluorescens HP72 and it’s associated with suppression of Creeping Bent grass (brown patch). Curr. Microbiol., 47: 138-143.
Taghavi, S., Garafola, C., Monchy, S., Newman, L. and Hoffman, A. (2009). Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees. Appl. Environ. Microbiol., 75: 748-757.
Yang, T., Law, D. M. and Davies, P. J. (1993). Magnitude and kinetics of stem elongation induced by exogenous indole-3-acetic acid in intact light-growth pea seedlings. Plant Physiol., 102: 717-724.
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