Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat
American Journal of BioScience
Volume 4, Issue 2, March 2016, Pages: 16-19
Received: Feb. 1, 2016; Accepted: Mar. 18, 2016; Published: May 3, 2016
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Monteomo Gnate François, Laboratory of Biochemistry, UFR-Med. Sciences, University Felix Houphouēt-Boigny, Abidjan, Côte d’Ivoire
Koffi Dago Pierre, Endocrinology and Diabetology Service, CHU-Yopougon-Abidjan, Abidjan, Côte d’Ivoire
Atto Virginie, Laboratory of Animal Physiology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, Côte d’Ivoire
Deh Zhou Patricia, Laboratory of Embryol-Cytol-Cytogen UFR-Med. Sciences-Univ F Houphouēt-Boigny, Abidjan, Côte d’Ivoire
Yapi Ahoua, Laboratory of Animal Biology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, Côte d’Ivoire
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Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.
Methodology, Traditional Cure, Oral Glucose, Wistar Rat
To cite this article
Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua, Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat, American Journal of BioScience. Vol. 4, No. 2, 2016, pp. 16-19. doi: 10.11648/j.ajbio.20160402.12
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Aké AL. Abrégés de médecine et pharmacopée ivoiriennes: quelques plantes employées, dans les soins de santé primaire. Editions NEI-CEDA. Abidjan, 2012, 86-94.
Anitha M, Sakthidevi G, Muthukumarasamy S, Mohan VR. Effect of cynoglossum zeylanicum (Vehl ex Hornem) Thinb. ex Lehm on oral glucose tolerance in rats. J of Applied pharmaceutical Science, 2012; 2, 11: 75-78.
Bauck. Basic anatomy, physiology, husbandry and clinical techniques. Part I. In Hillyer, Quesenberry. Ferrets, rabbits and roddents. Clin med and surg, saunders, 2004; 26, 291-297.
Debuigue G. Larousse des plantes qui guérissent. Librairie Larousse, 2010; p. 5-6.
Keita A, Mariko E, Haidara TK. Etude de l'activité hypoglycémiante des feuilles de Sclerocarya birrea (A. Rich) Hochst. (Anacardiaceae) Pharm. Méd. Trad. Afr. 1998, 10: 16-25.
Kerharo J, Adam JC. La pharmacopée sénégalaise traditionnelle: plantes médicinales et tropicales. Paris, Ed Vigot et frères, 1974; 433-35.
Konkon NG, Adjoungoua AL, Manda P. Toxicological and phytochemical screening study of Mitragyna inermis (Rubiaceae). Antidiabetic plant. J of medecinal plants research, 2008; 2, 10: 279-284.
Lokrou A. Guide de prise en charge des diabétiques. Collection santé, Editions Universitaires de Côte d’Ivoire, 2008; 212p.
Mohamed A, Tanko Y, Okasha MA. Effects of aqueous leaves extract of Ocimum gratissimum on blood glucose levels of streptozocin-induced diabetic Wistar rats. Afr J Biotechnolog, 2007; 6, 18: 2087-90.
Monteomo GF, Tehoua L, Ouattara H et al. Comparative antihyperglycemic activity of Sclerocarya birrea, Khaya senegalensis, Heliotropium indicum and Ocimum gratissimum to rats Wistar. Am J Biosciences, 2014; 2, 2: 60-63.
Monteomo GF. Valorisation de la pharmacopée africaine: étude de l’activité antihyperglycémique d’une préparation traditionnelle améliorée composée de sclerocarya birrea (Anacardiacée), Khaya senegalensis (Meliacée), Heliotropium indicum (Borraginacée) et Ocimum gratissimum (Lamiacée). Doctorat des Universités, Option, Biologie Humaine, Université FHB-Abidjan, UFR-SM, 2015; N° 26, 172p.
Nemlin J, Brunel JF. Travaux pratiques de matière médicale, 3ème année, Paris, Masson, 1995-1996: 39-43.
Ojewole JAO. Laboratory evaluation of the hypoglycemic effect of anarcadium occidentale Linn. (Anarcadiaceae) stem-bark extracts in rats. Methods and findings in experimental and clinical, 2003; 25, 3: 199-204.
Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychnos henningsii Gild in streptozotocin-nicotinamide types 2 diabetic rats. J of Pharmaceutical Research, 2012; 11, 1: 221-228.
Vilmer JB. Ethique animale. Que sais-je. Presses universitaires de France, Paris, 2015; 79-90p.
Zhao VW, James MU, Schraw TD. Panic-Attac: A Mouse model for Inducible and Reversible β-Cell Ablation. Diab. 2008; 57, 8: 2137-2148.
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