Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria
American Journal of BioScience
Volume 4, Issue 5, September 2016, Pages: 58-63
Received: Apr. 25, 2016; Accepted: Aug. 25, 2016; Published: Sep. 12, 2016
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Gidado Rose Suniso Maxwell, Agricultural Biotechnology Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria;Department of Biological Sciences, University of Abuja, Abuja, Nigeria
Etim Veronica Archibong, Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Nweke Ogechi, Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Iloh Andrew Chibuzor, Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria
Isu Rosemary Nennaya, Department of Biological Sciences, University of Abuja, Abuja, Nigeria
Solomon Bamidele Ogbe, Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria
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Two yeast strains referred to as OY and SY were isolated and characterised from local distillery and cereal milling sites. Isolation was done using potato dextrose media supplemented with 0.2% chloramphenicol. Morphological and biochemical results together with the rDNA internal transcribed spacer region (ITS) were identified as Pichia kudriavzevii strains GY1 and L9 respectively. OY and SY are ethanol tolerant strains, withstanding ethanol concentrations of up to 20% (v/v) in yeast extract, peptone, malt extract, glucose media. OY and SY displayed good growth in ethanol supplemented medium with pH ranging from 4.8-5.5 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The results obtained suggested that OY and SY demonstrated good parameters as ideal candidates for bioethanol production.
Ethanol, Ethanol Tolerance, Saccharomyces, Non-saccharomyces, Pichia kudriavzevii
To cite this article
Gidado Rose Suniso Maxwell, Etim Veronica Archibong, Nweke Ogechi, Iloh Andrew Chibuzor, Isu Rosemary Nennaya, Solomon Bamidele Ogbe, Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria, American Journal of BioScience. Vol. 4, No. 5, 2016, pp. 58-63. doi: 10.11648/j.ajbio.20160405.11
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Farrell, A. E., Plevin, R. J., Turner, B. T., Jones, A. D., O'Hare, M., and Kammen, D. M. (2006). Ethanol can contribute to energy and environmental goals. Science, 311: 506- 508.
Davis, L., Rogers, P., Pearce, J., and Peiris, P. (2006). Evaluation of Zymomonas based ethanol production from a hydrolysed waste starch stream. Biomass and Bioenergy, 30: 809–814.
Edgardo, A., Parra, C., Manuel, R., Juanita, F., Baeza, J. (2008). Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production. Enzyme and Microbial Technology, 43: 120-123.
Signori, L., Passolunghi, S., Ruohonen, L., Porro, D., and Branduardi P. (2014). Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain. Microbial Cell Factories, 13: 51-64.
Cakar Z., Seker U., Tamerler C., Sonderegger, M., and Sauer, U. (2005). Evolutionary engineering of multiple-stress resistant Saccharomyces cerevisiae. FEMS Yeast Research, 5: 569–578.
Jolly, N. P., Augustyn, O. P. H., and Pretorius, I. S. (2003). The effect of non-Saccharomyces yeasts on fermentation and wine quality. South African Journal of Enology and Viticulture, 24: 55-62.
Matsushika A., Inoue, H., Kodaki, T., and Sawayama, S. (2009). Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives. Applied Microbiology and Biotechnology, 84: 37–53.
Ciani, M., Comitini, F., Mannazzu, I., and Domizio, P. (2010). Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in win¬emaking. FEMS Yeast Research, 10: 123–133.
Li, J. R., and Cai, A. Q. (2007). Isolation and identification of main microorganisms in traditional distiller's yeast. Liquor-Making Science and Technology, 5: 111-115.
Tao N., Gao, Y., and Liu, Y., (2011). Isolation and characterization of a Pichia anomala strain: a promising candidate for bioethanol production. Brazilian Journal of Microbiology, 42: 668-675.
Cocolin, L., Bisson, L. F., and Mills, D. A. (2000). Direct profiling of the yeast dynamics in wine fermentations. FEMS Microbiology Letters, 189: 81-87.
Tofalo, R., Chaves-Lopez, C., Fabio, F. D., Schirone, M., Felis, G. E., Torriani, S., et al. (2009). Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must. International Journal of Food Microbiology, 130: 179-187.
Bhima, B., Marrivada, S. R., Devi, T. A., Reddy, Y. R., and Rao, L. V. (2010). Screening and characterization of stress tolerant Saccharomyces Cerevisiae isolated from brewery effluents for animal probiotic applications. IIOAB Research: Food Bio-technology, 1: 32-38.
Barnett, J. A., Payne, R. W., and Yarrow, D. (2000). Yeasts: Characteristics and identification. 3rd edn. Cambrie University Press, UK. ISBN-13: 978-0521573962.
Khaing, T. W., Weine, N., and Mya, MO. (2008). Isolation, Characterization and Screening of Thermo tolerant, Ethanol Tolerant Indigenous Yeasts and Study on the Effectiveness of Immobilized Cell for Ethanol Production. Journal of Science and Technology, 1: 12-14.
Caggia, C., Restuccia, C., Pulvirenti, A., and Giudici, P. (2001). Identification of Pichia anomala isolated from yoghurt by RFLP of the ITS region. International Journal of Food Microbiology, 71: 71-73.
Lee, Y. J., Choi, Y. R., Lee, S. Y., Park, J. T., Shim, J. H., Park, K. H., and Kim, J. W. (2011). Screening Wild Yeast Strains for Alcohol Fermentation from Various Fruits. Mycobiology 39: 33-39.
Barrio, E., González, S. S., Arias, A., Belloch, C., and Querol, A. (2006). Molecular mechanisms involved in the adaptive evolution of industrial yeasts. In: Yeasts in Food and Beverages (Eds: Querol A, Fleet G). Springer-Verlag, Berlin, Germany. pp 153-173.
Chen, Y. C., Eisner, J. D., Kattar, M. M., Rassoulian-Barrett, S. L., LaFe, K., Yarfitz, S. L., Limaye, A. P., and Cookson, B. T. (2000). Identification of medically important yeast using PCR-based detection of DNA sequence polymorphism in the internal transcribed spacer 2 region of the rRNA genes. Journal of Clinical Microbiology, 38: 2302-2310.
Desnos-Ollivier, M., Ragon, M., Robert, V., Raoux, D., Gantier, J. C., and Domer, F. (2008). Debaryomyces hansenii (Candida famata), a rare human fungal pathogen often misidentified as Pichia guilliermondii (Candida guilliermondii). Journal of Clinical Microbiology, 46: 3237-3242.
Mukherjee, V., Steensels, J., Lievens, B., et al. (2014). Phenotypic evaluation of natural and industrial Saccharomyces yeasts for different traits desirable in industrial bioethanol production. Applied Microbiology and Biotechnology, 98: 9483–9498.
Walker, L. P., Hii, H., and Wilson, D. B. (2006). Enzymatic hydrolysis of cellulose: An Overview. Bioresources Technology, 36: 3-14.
Lei, J. J., Zhao, X. Q., Ge, X. M., and Bai, F. W. (2007). Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution. Journal of Biotechnology, 131: 270-275.
Rajoka, M., Khalid A, and Ferhan, M. (2005). Kinetic and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae. Letters in Applied Microbiology, 40: 316–21.
Wang, Z. X., Zhuge, J., Fang, H., and Prior, B. A. (2001). Glycerol production by microbial fermentation: A review. Biotechnology Advances, 19: 201-223.
Thomas, K. C., Hynes, S. H. and Ingledew, W. M. (2002). Influence of medium buffering capacity on inhibition of Saccharomyces cerevisiae growth by acetic and lactic acids. Applied and Environmental Microbiology, 68: 1616-1623.
Ruyters, S., Mukherjee, V., Verstrepen, KJ., et al. (2015). Assessing the potential of wild yeasts for bioethanol production. Journal of Industrial Microbiology and Biotechnology, 42: 39–48.
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