Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film
American Journal of BioScience
Volume 5, Issue 5, September 2017, Pages: 74-79
Received: Jun. 10, 2017; Accepted: Jun. 22, 2017; Published: Aug. 30, 2017
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Author
Temam Abrar Hamza, Department of Biotechnology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia
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Abstract
Proteases are multipurpose group of enzymes which mainly produced from microorganism and used in various industries such as detergent, silver recovery, food, pharmaceutical, leather and textile industries. This work aimed to isolate protease producing bacteria from local environment for use as silver recovery and reuse of polyester from waste X-ray film. Isolation of protease producer was undertaken using skim milk agar medium. A total of 36 protease positive bacteria were isolated from the study area (Arba Minch fish processing site). Five isolates were screened which had relatively higher clear zone and further examined gelatin removal efficiency of their crude enzymes. One isolate designated as Bacillus sp. THZ14 was selected on the basis of gelatin removal efficiency. The crude enzyme from this strain removed gelatin on waste X-ray film at pH 9, 40°C in 40 min. These properties suggest that protease from Bacillus sp. THZ14 could find potential application in silver recovery process and reuse of polyester sheet which have an economic implication.
Keywords
Bacillus sp. THZ14, Enzyme, Gelatin Hydrolysis, Protease, X-ray Films
To cite this article
Temam Abrar Hamza, Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film, American Journal of BioScience. Vol. 5, No. 5, 2017, pp. 74-79. doi: 10.11648/j.ajbio.20170505.11
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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