American Journal of BioScience

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Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp

Received: 08 October 2019    Accepted: 26 October 2019    Published: 31 October 2019
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Abstract

Cellulases have diversity of industrial applications and their cost effective production using agroindustrial wastes by solid state fermentation poses an efficient method. Actinomycetes are considered highly valuable due to their secondary metabolites production and in this study, an attempt was made to optimize the use of ragi husk and refine the process of cellulase production by temperature mediated solid state fermentation. Actinomycetes were isolated from paper mill industry soil and cellulase producing Streptomyces sp was selected for the experiments. Ragi husk was used as substrate for solid state fermentation of cellulase and varying incubation temperatures (20°C, 25°C, 30°C, 35°C and 40°C) was considered to determine its effect on enzyme activity after 6th, 9th and 12th day of fermentation. The carboxymethyl cellualse (CMC-ase) activity was measured and the observations obtained were compared with the standard glucose curve to determine the amount of reducing sugar (µg ml-1) released. Enzyme activity was highest at 35°C and was recorded as 35.14, 45.90 and 59.56 IU ml-1 at the end of 6th, 9th and 12th day of fermentation. Highest amount of reducing sugars at a concentration of 322 µg ml-1 was released at the end of 12th day at 35°C. The results indicated that the enzyme activity was temperature dependent while using ragi husk as growth substrate under solid state fermentation.

DOI 10.11648/j.ajbio.20190704.11
Published in American Journal of BioScience (Volume 7, Issue 4, July 2019)
Page(s) 77-81
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cellulase, Streptomyces, Solid State Fermentation, Agroindustrial Wastes, Ragi Husk, Actinomycetes

References
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Author Information
  • Department of Biology, Education Faculty, Jawzjan University, Sheberghan City, Afghanistan

  • Department of Biotechnology, Indian Academy Degree College-Autonomous, Bengaluru, India

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    Totiya Ishchi, Sibi G. (2019). Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp. American Journal of BioScience, 7(4), 77-81. https://doi.org/10.11648/j.ajbio.20190704.11

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    Totiya Ishchi; Sibi G. Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp. Am. J. BioScience 2019, 7(4), 77-81. doi: 10.11648/j.ajbio.20190704.11

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    Totiya Ishchi, Sibi G. Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp. Am J BioScience. 2019;7(4):77-81. doi: 10.11648/j.ajbio.20190704.11

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  • @article{10.11648/j.ajbio.20190704.11,
      author = {Totiya Ishchi and Sibi G.},
      title = {Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp},
      journal = {American Journal of BioScience},
      volume = {7},
      number = {4},
      pages = {77-81},
      doi = {10.11648/j.ajbio.20190704.11},
      url = {https://doi.org/10.11648/j.ajbio.20190704.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbio.20190704.11},
      abstract = {Cellulases have diversity of industrial applications and their cost effective production using agroindustrial wastes by solid state fermentation poses an efficient method. Actinomycetes are considered highly valuable due to their secondary metabolites production and in this study, an attempt was made to optimize the use of ragi husk and refine the process of cellulase production by temperature mediated solid state fermentation. Actinomycetes were isolated from paper mill industry soil and cellulase producing Streptomyces sp was selected for the experiments. Ragi husk was used as substrate for solid state fermentation of cellulase and varying incubation temperatures (20°C, 25°C, 30°C, 35°C and 40°C) was considered to determine its effect on enzyme activity after 6th, 9th and 12th day of fermentation. The carboxymethyl cellualse (CMC-ase) activity was measured and the observations obtained were compared with the standard glucose curve to determine the amount of reducing sugar (µg ml-1) released. Enzyme activity was highest at 35°C and was recorded as 35.14, 45.90 and 59.56 IU ml-1 at the end of 6th, 9th and 12th day of fermentation. Highest amount of reducing sugars at a concentration of 322 µg ml-1 was released at the end of 12th day at 35°C. The results indicated that the enzyme activity was temperature dependent while using ragi husk as growth substrate under solid state fermentation.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Ragi Husk as Substrate for Cellulase Production Under Temperature Mediated Solid State Fermentation by Streptomyces Sp
    AU  - Totiya Ishchi
    AU  - Sibi G.
    Y1  - 2019/10/31
    PY  - 2019
    N1  - https://doi.org/10.11648/j.ajbio.20190704.11
    DO  - 10.11648/j.ajbio.20190704.11
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 77
    EP  - 81
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20190704.11
    AB  - Cellulases have diversity of industrial applications and their cost effective production using agroindustrial wastes by solid state fermentation poses an efficient method. Actinomycetes are considered highly valuable due to their secondary metabolites production and in this study, an attempt was made to optimize the use of ragi husk and refine the process of cellulase production by temperature mediated solid state fermentation. Actinomycetes were isolated from paper mill industry soil and cellulase producing Streptomyces sp was selected for the experiments. Ragi husk was used as substrate for solid state fermentation of cellulase and varying incubation temperatures (20°C, 25°C, 30°C, 35°C and 40°C) was considered to determine its effect on enzyme activity after 6th, 9th and 12th day of fermentation. The carboxymethyl cellualse (CMC-ase) activity was measured and the observations obtained were compared with the standard glucose curve to determine the amount of reducing sugar (µg ml-1) released. Enzyme activity was highest at 35°C and was recorded as 35.14, 45.90 and 59.56 IU ml-1 at the end of 6th, 9th and 12th day of fermentation. Highest amount of reducing sugars at a concentration of 322 µg ml-1 was released at the end of 12th day at 35°C. The results indicated that the enzyme activity was temperature dependent while using ragi husk as growth substrate under solid state fermentation.
    VL  - 7
    IS  - 4
    ER  - 

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