Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening
American Journal of BioScience
Volume 8, Issue 1, January 2020, Pages: 15-19
Received: Mar. 6, 2020;
Accepted: Mar. 31, 2020;
Published: Apr. 13, 2020
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Genco Francesca, Microbiology and Virology Unit, Fondazione Istituto di Ricerca e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
Meroni Valeria, Department of Internal Medicine and Medical Therapy, University of Pavia, Pavia, Italy
De Silvestri Annalisa, Clinical Epidemiology and Biostatistics Unit, Fondazione Istituto di Ricerca e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
Bouthry Elise, Lab. CERBA Saint-Ouen-l'Aumône, Paris, France
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Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON® XL DiaSorin, Abbott Architect and Roche Cobas®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON® assay was better than both the Cobas® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas®.
Toxoplasma gondii, Cytomegalovirus, Prenatal Screening, Anti-cytomegalovirus IgG Antibodies, Anti-toxoplasma IgG Antibodies
To cite this article
De Silvestri Annalisa,
Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening, American Journal of BioScience.
Vol. 8, No. 1,
2020, pp. 15-19.
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Hide G. Role of vertical transmission of Toxoplasma gondii in prevalence of infection. Expert Rev Anti Infect Ther 2016.
Kenneson A and Cannon MJ. Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection. Rev Med Virol 2007; 17 (4): 253-276.
Lewis JM, Clifford S, et al. Toxoplasmosis in immunosuppressed patients. Rheumatology (Oxford) 2015; 54 (11): 1939-1940.
Sissons JG and Wills MR. How understanding immunology contributes to managing CMV disease in immunosuppressed patients: now and in future. Med Microbiol Immunol 2015; 204 (3): 307-316.
Neu N, Duchon J, et al. TORCH infections. Clin Perinatol 2015; 42 (1): 77-103, viii.
McAuley JB. Congenital Toxoplasmosis. J Pediatric Infect Dis Soc 2014; 3 Suppl 1: S30-35.
Sterkers Y, Pratlong F, et al. Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection. J Clin Microbiol 2012; 50 (12): 3944-3951.
Adams Waldorf KM and McAdams RM. Influence of infection during pregnancy on fetal development. Reproduction 2013; 146 (5): R151-162.
Fowler KB and Boppana SB. Congenital cytomegalovirus (CMV) infection and hearing deficit. J Clin Virol 2006; 35 (2): 226-231.
Delforge ML, Desomberg L, et al. Evaluation of the new LIAISON ((R)) CMV IgG, IgM and IgG Avidity II assays. J Clin Virol 2015; 72: 42-45.
Montoya JG. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 2002; 185 Suppl 1: S73-82.
Wu D, Wu Y, et al. Evaluation of a novel array-based toxoplasma, rubella, cytomegalovirus, and herpes simplex virus IgG enzyme linked immunosorbent assay and its comparison with virion/serion enzyme linked immunosorbent assays. Ann Lab Med 2014; 34 (1): 38-42.
Petersen E, Borobio MV, et al. European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol 2005; 43 (4): 1570-1574.
Khan K, Khan W. 2018. Congenital toxoplasmosis: an overview of the neurological and ocular manifestations. Parasitology International, 67, 715–721.