Production of Amylase from Bacteria Isolated from Tannery Effluent
American Journal of BioScience
Volume 8, Issue 2, March 2020, Pages: 41-44
Received: Nov. 6, 2019; Accepted: Nov. 23, 2019; Published: May 19, 2020
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Authors
Oyeleke Solomon Bankole, Department of Microbiology, Federal University of Technology, Minna, Nigeria
Erena Nuhu Bako, Integrated Science Department, College of Education, Minna, Nigeria
Manga Shuaibu Bala, Department of Microbiology, Usumanu Danfodiyo University, Sokoto, Nigeria
Manga Sahabi, Department of Biological Sciences, Kebbi State University of Technology, Alero, Nigeria
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Abstract
Bacteria isolated from tannery effluent collected from Erena local tannery in Niger State were examined for amylase production. Two of the isolates, Bacillus subtilis and Psuedomonas aeruginosa demonstrated ability of amylase production, where Bacillus subtilis has it highest amylase activity of 2.1mg/ml at 18hr, while Psuedomonas aerugionsa has highest activity of 1.7mg/ml at the same period. The optimum temperature and pH were also determined, Bacillus subtilis has highest amylase activity of 2.5mg/ml at 60°C, while Psuedomonas aeruginosa has highest activity of 2.3mg/ml at 80°C. The optimum pH for amylase produced by Bacillus subtilis and Psuedomonas aeruginosa at pH 7 were found to be 2.8mg/ml and 2.7mg/ml respectively. Streptococcus faecalis was not able to produce amylase. The research recommended that, production of the enzymes (amylase) from the two bacteria (Pseudomonas aeruginosa and Bacillus subtilis) will pave way for industrialization and reduced cost of production and procurement of this enzymes. The production of amylase by Psuedomonas aeruginosa and Bacillus subtilis will also go a long way to conserve our hard earn foreign exchange thereby enhancing the economy, if these orgasms are fully exploited for the production of the enzyme, as well as utilizing the waste effluent that can pollute the environment to a beneficial aspect.
Keywords
Tannery, Effluents, Amylase, Bacteria, Isolates
To cite this article
Oyeleke Solomon Bankole, Erena Nuhu Bako, Manga Shuaibu Bala, Manga Sahabi, Production of Amylase from Bacteria Isolated from Tannery Effluent, American Journal of BioScience. Vol. 8, No. 2, 2020, pp. 41-44. doi: 10.11648/j.ajbio.20200802.13
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Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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