Production of Amylase from Bacteria Isolated from Tannery Effluent
American Journal of BioScience
Volume 8, Issue 2, March 2020, Pages: 41-44
Received: Nov. 6, 2019;
Accepted: Nov. 23, 2019;
Published: May 19, 2020
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Oyeleke Solomon Bankole, Department of Microbiology, Federal University of Technology, Minna, Nigeria
Erena Nuhu Bako, Integrated Science Department, College of Education, Minna, Nigeria
Manga Shuaibu Bala, Department of Microbiology, Usumanu Danfodiyo University, Sokoto, Nigeria
Manga Sahabi, Department of Biological Sciences, Kebbi State University of Technology, Alero, Nigeria
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Bacteria isolated from tannery effluent collected from Erena local tannery in Niger State were examined for amylase production. Two of the isolates, Bacillus subtilis and Psuedomonas aeruginosa demonstrated ability of amylase production, where Bacillus subtilis has it highest amylase activity of 2.1mg/ml at 18hr, while Psuedomonas aerugionsa has highest activity of 1.7mg/ml at the same period. The optimum temperature and pH were also determined, Bacillus subtilis has highest amylase activity of 2.5mg/ml at 60°C, while Psuedomonas aeruginosa has highest activity of 2.3mg/ml at 80°C. The optimum pH for amylase produced by Bacillus subtilis and Psuedomonas aeruginosa at pH 7 were found to be 2.8mg/ml and 2.7mg/ml respectively. Streptococcus faecalis was not able to produce amylase. The research recommended that, production of the enzymes (amylase) from the two bacteria (Pseudomonas aeruginosa and Bacillus subtilis) will pave way for industrialization and reduced cost of production and procurement of this enzymes. The production of amylase by Psuedomonas aeruginosa and Bacillus subtilis will also go a long way to conserve our hard earn foreign exchange thereby enhancing the economy, if these orgasms are fully exploited for the production of the enzyme, as well as utilizing the waste effluent that can pollute the environment to a beneficial aspect.
Tannery, Effluents, Amylase, Bacteria, Isolates
To cite this article
Oyeleke Solomon Bankole,
Erena Nuhu Bako,
Manga Shuaibu Bala,
Production of Amylase from Bacteria Isolated from Tannery Effluent, American Journal of BioScience.
Vol. 8, No. 2,
2020, pp. 41-44.
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
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Ajayi, A. O. and Fagade O. E. (2006) Growth pattern and structural nature of amylases produced by some Bacillus species in starchy substrate. Africa journal of Biotechnology. 5 (5); 440-444.
Ali G. A. O. (1992). Formation of protease by Aspergillums fumigates and Penicillium spp. Journal of King Saad University of science 4 (2); 127.
APHA (1995). Standard methods., standard method for the examination of water and wastewater. American public Health Association American water works Association and waters environment federation Eaton Ad, Clesceri LS, Greenberg AE.
Bertrand T. F., Fredric T. and Robert, N. (2004), Production and partial characterization of a thermostable amylase from Ascomycetes yeast strain isolated from starchy soil. McGraw Hill Inc. York Pp 53-55.
Burham, A., Nisa U., Gokhan C., Ashabil A., and Osmar G. (2003). Enzymatic properties of award thermostable thermophilic alkaline and chekato resistant amylase from an alkaphilic Bacillus spp isolate ANT-6. Proceedings of Biochemistry; (38) 1397-1403.
Cordeiro, C. A. M. Martinas M. I. L. and Lucaino A. (2003). Production and properties of alpha amylase from thermophylic Bacillus specie. Brazilian journal of microbiology (933) 1-3.
Daniel, R. M., Peterson M. E. and Dauson M. J. (2010). The molecular basis of the effect of temperature on enzyme activity. Journal of Biochemistry. 425 (2) 353-360.
Frolund, B. Palmgren R., Keiding K. and Nielsen PH. (1995) Enzymatic activity in the activity in the activated sludge floe matrix. Applied Microbial Biotechnology (43) 708–16.
Gupta A., Gupta V. K.., Modi D. R. and Yadava L. P. (1995). Production and characterization of a-amylase from Aspergillus niger, Journal of Biotechnology 7: 551-556.
Modi, H. A. Patel K. C., and Ray R. M. (1994). In solid state fermentation (ed pandey wiley Eastern publishes, New Delhi pp 137-141.
Oyeleke S. B and Manga S. B. (2008) Essential of laboratory practicals in Microbiology (1sted Tobest publisher, Niger State pp 36-58.
Oyeleke S. B. and Oduwale A. A. (2009) Production of Amylase by bacteria isolated form a cassava waste dumpsite in Minna, Niger, Nigeria. Africa Journal of Microbial Research 3 (4) 143-146.
Oyeleke S. B., Auta S. H, and Egwin E. C. (2010). Production and characterization of amylase produced by Bacillus megaterium, from a Local yam peel dumpsite in Minna, Niger State. Journal of microbiology and Antimicrobials 2 (7) Pp 88-92.
Oyeleke S. B., Auta S. H and Egwim E. C. (2011). Screening of Aspergillus flavus and Aspergillus fumigatus strains for extracellular protease enzyme production. journal of Microbiology and Antimicrobials 2 (7) Pp 83-87.