Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture
American Journal of BioScience
Volume 8, Issue 3, May 2020, Pages: 57-64
Received: Sep. 18, 2019; Accepted: Oct. 4, 2019; Published: May 28, 2020
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Authors
Sandanakirouchenane Aroumougame, Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, India
Thirumangai Mannan Geetha, Department of Agricultural Microbiology, Adhiparasakthi Agricultural College, Kalavai, India
Muthu Thangaraju, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, India
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Abstract
Although many bacterial species have been isolated from the rhizosphere of various crop plants, the recent discovery is Burkholderia sp., an endophytic bacterium. In this study, the Burkholderia isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were enumerated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and black gram using N-free BAz (Burkholderia Azelaic acid) medium, in which black gram roots were observed higher population. Further, growth promoting activities of the Burkholderia isolates were examined, the maximum production of IAA and GA was noticed on the BB4 as compared to other isolates and the cytokinin production was recorded more in isolates SB3 followed by BB4. Among the four isolates, maximum amount of salicylate type was noticed in RB1 and catechol type was recorded higher in BB4 which showed that these isolates were capable to produce Siderophore. The ACC deaminase activity of the isolates were exhibited, the BB4 was recorded more followed by SB3. Therefore, the endophytic Burkholderia isolates also the important contributor to the crop growth through secretion of growth promoting substances, production of siderophore and ACC deaminase activities may improve the Agriculture production.
Keywords
Burkholderia Isolates, Endophytes, Indole Acetic Acid, Gibberellic Acid, Cytokinin, Siderophore, ACC Deaminase
To cite this article
Sandanakirouchenane Aroumougame, Thirumangai Mannan Geetha, Muthu Thangaraju, Exploitation of PGPR Endophytic Burkholderia Isolates to Enhance Organic Agriculture, American Journal of BioScience. Vol. 8, No. 3, 2020, pp. 57-64. doi: 10.11648/j.ajbio.20200803.12
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Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Hiltner, L. Iiber neuere Erfahrungen und unter besonderer Berucksichtingung der Grundunging und Brache. Arb. Dtsch. Landw. Ges, 1904, 98; 59-78.
[2]
Smalla, K., Sessitsch, A., and Hartmann, A. The rhizosphere: soil compartment influenced by the root. Fed. Eur. Microbiol. Soc, 2006, 56; 165–172.
[3]
Davison, J. Plant beneficial bacteria. Biotechnol, 1988, 6; 282-286.
[4]
Kloepper, J. W., Lifshitz, R., and Zablotowicz, R. M. Free-living bacterial inocula for enhancing crop productivity. Trends Sci, 1989, 1; 60-64.
[5]
Weller, D. M., and Thomashow, L. S. Current challenges in introducing beneficial microorganisms into the rhizosphere. In: Molecular Ecology of Rhizosphere Microorganisms (eds.) F. Dowling, D. N. and B. Boesten, VCH Verlagsgesellschaft mbH, Weinheim, Germany, 1994, pp. 10-18.
[6]
Glick, B. R. The enhancement of free-living bacteria. Can. J. Microbiol, 1995, 41; 109-117.
[7]
Probanza, A., Lucas, J. A., Acero, N., and Gutierrez-Manero, F. J. The influence of native bacteria on European alder (Alnus glutinosa (L.) Gaertn.) growth. Characterization of growth promoting and growth inhibiting bacterial strains. Plant Soil, 1996, 182; 59-66.
[8]
Suresh, A., Pallavi, P., Srinivas, P., Praveen Kumar, V., Jeevan Chandra, S., R et al. Plant growth promoting activities of fluorescent pseudomonads associated with some crop plants. Afr. J. Microbiol. Res, 2010, 4 (14); 1491-1494.
[9]
Bernard Glick, R. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res, 2014, 169; 30– 39.
[10]
Sang Hye Jia, Mayank Anand Gururanib, and Se-Chul, C. Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars. Microbiol. Res, 2014, 169; 83–98.
[11]
Premachandra, D., Hudek, L., and Brau, L. Bacterial Modes of Action for Enhancing of Plant Growth. J. Biotech. Biomater, 2016, 6; 3.
[12]
Burkholder, W. Sour skin, a bacterial rot of onion bulbs. Phytopathol, 1950, 40; 115-118.
[13]
Barraquio, W. L., L. Sevilla and J. K. Ladha. Isolation of endophytic diazotrophic bacteria from wetland rice. Plant Soil, 1997, 194; 15-24.
[14]
Baldani, J. I., L. Caruso, V. L. D Baldani, S. R. Goi and J. Dobereiner. Recent advances in BNF with non-legume plants. Soil. Biol. Biochem, 1997, 29; 911-922.
[15]
Vandamme, P., Henry, D., Coenye, T., Nzula, S., Vancanneyt, M., L. et al. Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound test results of new molecular diagnostic tools. FEMS Immunol. Med. Microbiol, 2002, 33; 143-149.
[16]
Estrada., De-Los-Santos, E., R. Bustillos-Cristales and J. Caballero-Mellado. Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl. Environ. Microbiol, 2001, 67; 2790-2798.
[17]
Arora, N. K., Kang, S. C., and Maheshwari, D. K. Isolation of siderophore-producing strains of Rhizobium meliloti and their biocontrol potential against Marcrophomina phaseolina that causes charcoal rot of groundnut. Curr. Sci, 2001, 81; 673–7.
[18]
Persello-Cartieaux, F., Nussaume, L., and Robaglia, C. Tales from the underground: molecular plant-rhizobacteria interactions. Plant Cell Environ, 2003, 26; 189-199.
[19]
Kuklinsky-Sobral, J., Araujo, W. L., Mendes, R., Geraldi, I. O., Pizzirani-Kleiner, A. A., A. et al. Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. Envirion. Microbiol, 2004, 6; 1244–51.
[20]
Frey-Klett, P., Chavatte, M., Clausse, M. L., Courrier, S., Roux, C. L., R. et al. Ecto-mycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads. New Phytol, 2005, 165; 317–28.
[21]
Hameeda, B., Rupela, O. P., Reddy, G., and Satyavani, K. Application of plant growth promoting bacteria associated with composts and macro fauna for growth promotion of pearl millet (Pennisetum glaucum L.). Biol. Fertil. Soils, 2006, 43; 221–227.
[22]
Caballero-Mellado, J., L. Martinez-Aguilar, G. Paredes-Valdez and P. Estrada de- lost Santos. Burkholderia unamae sp. nov., a N2 fixing rhizospheric and endophytic species. Int. J. Syst. Environ. Microbiol, 2004, 10; 1-12.
[23]
Honma, M., and Shimomura, T. Metabolism of 1-aminocyclopropane-1-carboxylic acid. Agric. Biol. Chem, 1978, 42; 341-346.
[24]
Bakshi, A., Shemansky J. M., Chang, C., and Binder, B. M. History of research on the plant hormone ethylene. J. Plant Growth Regul, 2015, 34; 809–827.
[25]
Shrivastava, P., and Kumar, R. Soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi J. Biol. Sci, 2015, 22; 123–131.
[26]
Sandanakirouchenane, A., Ekramul Haque, and Geetha, T. Recent Studies on N2 Fixing Burkholderia Isolates as a Biofertilizer for the Sustainable Agriculture. Int. J. Curr. Microbiol. App. Sci, 2017, 6 (11); 2780-2796.
[27]
Hallmann, J., Quadt-Hallmann, A., Mahafee, W. F., and Kloepper, J. W. Bacterial endophytes in agricultural crops. Can. J. Microbiol, 1997, 43; 895-914.
[28]
Tien, T. M., M. H. Gasking and D. H. Hubbel. Plant growth substances produced by A. brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl. Environ. Microbiol, 1979, 37; 1016-1024.
[29]
Gorden, S. A., and Paleg, L. G. Quantitative measurements of indole acetic acid. Physiol. Pl, 1957, 4; 24-27.
[30]
Zeigler, R., Powell, L., and Thurston, H. Gibberellin A4 Production by Sphaceloma manihoticola, Causal Agent of Cassava Super Elongation Disease. Physio. Biochem, 1980, 70; 589-593.
[31]
Reeves, M. O., Neilands, P. L., and Ballows, A. Absorption of siderophore activity Legionella sp. in iron deficient media. J. Bacteriol, 1983, 154; 324-329.
[32]
Penrose, D. M. and B. R. Glick. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol. Pl, 2003, 118; 10-15.
[33]
Estrada, P., P. Mavingui, B. Cournoyer, F. Fontaine, J. Balandreau,. C. et al. Nitrogen fixing endophytic Burkholderia sp. associated with maize plants cultivated in Mexico. Can. J. Microbiol, 2002, 48; 285-294.
[34]
Vogel, J. L, Balandreau, J., Van Antwerpen, T., Dana, P., and Cadet, P. Isolation and characterization of sugarcane rhizobacteria and their effect on nematodes. Proc. S. Afr. Sug. Technol. Ass, 2002, 76; 305-309.
[35]
Tran Van, V., P. Mavingui, O. berge, J. Balandreau and T. Heulin. Promotion de croissance du riz inocule par une bacterie flxatrice d’azote, Burkholderia vietnamiensis, isolee d’un sol sulfate acide duviet-nam. Agronomic, 2002, 14; 697-707.
[36]
Neto, R., Malavolta, J. V. A., and Vicot, O. Suma Phytopatologica, 1986, 12; 16.
[37]
Tisha, P., and Meenu, S. Biosynthesis of phytohormones from novel rhizobacterial isolates and their in vitro plant growth-promoting efficacy. J. Pl. Interac, 2017, 12 (1); 480–487.
[38]
Nita, A. Z. The activity of metabolic products of microorganisms on higher plants. Paper presented at the VIII. Intern. Congr. Soil. Sci. Commission III. Bucharest, 1964, p. 106-107.
[39]
Eklund, E. Secondary effects of some pseudomonads in the rhizoplane of peatgrown cucumber plants. Acta. Agr. Second Supple, 1970, 17; 1-57.
[40]
Jain, D. K., and Patriquin, D. G. Characterization of a substance produced by a Azospirillum which causes branching of wheat root hair. Can. J. Microbiol, 1985, 31; 206-210.
[41]
Esparza, M. A., Villa-Gonzalez, R., and Caballero-Mellado, J. Acetylene reduction and indoleacetic acid production by Azospirillum isolates from cactaceous plants. Plant Soil, 1988, 106; 91-95.
[42]
Eman, A. A., Enas, A. H., El Tobgy, K. M. K., and Ramadan, E. M. Evaluation of rhizobacteria of some medicinal plants for plant growth promotion and biological control. Ann. Agri. Sci, 2014, 59 (2); 273–280.
[43]
Sagar A. Desai, Isolation and characterization of gibberellic acid (GA3) producing rhizobacteria from sugarcane roots. Biosci. Discovery, 2017, 8 (3); 488-494.
[44]
Arthee, R., and Marimuthu, P. Studies on Endophytic Burkholderia Sp. from sugarcane and its screening for Plant Growth Promoting potential. J. Exp. Biol. Agri. Sci, 2017, 5 (2); 242-257.
[45]
Shamakharora, N. M. Alfalfa root hair deformation caused by growth substances and Rhizobium meliloti culture fluid extracts. Microbiology. 1979, 48; 276-279.
[46]
Barea, J. M., and Brown, M. E. Effects on plant growth produced by Azotobacter paspali related to synthesis of plant growth regulating substances. J. Appl. Bacteriol, 1974, 37 (4); 583-93.
[47]
Reynders, L., and Vlassak, K. Conversion of tryptophan to indole acetic acid by Azospirillum brasilense. Soil. Biol. Biochem, 1979, 11; 547-548.
[48]
Azcon, G., Agullar, C., and Barea, J. M. Effects of interactions between different culture fractions of phosphobacteria and Rhizobium on mycorrhizal infection, growth and nodulation of Medicago sativa. Can. J. Microbiol, 1978, 24; 520-524.
[49]
Brown, M. E. Plant growth substances produced by microorganisms of soil and rhizosphere. J. Appl. Bacteriol, 1972, 68; 1377-1383.
[50]
Mordukhova, E. A., Skvortsova, N. P., Kochetkov, V. V., Dubeikovsky, A. N., and Boronin, A. M. Indole-3-acetic acid, a phytohormone synthesized by the rhizosphere bacteria of the Pseudomonas genus. Mikrobiologia, 1991, 60; 494-500.
[51]
Govind, G., Shailendra, S. P., Narendra Kumar, A., Sunil Kumar, S., and Vinod Singh. Plant Growth Promoting Rhizobacteria (PGPR): Current and Future Prospects for Development of Sustainable Agriculture. Microbiol. Biochem. Technol, 2015, 7 (2); 96-102.
[52]
Boukhalfa, H., and Crumbliss, A. L. Chemical aspects of siderophore in iron transport. Biometals, 2002, 15; 325-339.
[53]
Darling, P., Chan, M., Cox, A. D., and Sokol, P. A. Siderophore production by cystic fibrosis isolates of Burkholderia cepacia. Infect Immun, 1998, 66; 874-877.
[54]
Stephan, H., Freund, S., Beck, W., Jung, G., Meyer, J. M., W. et al. Ornibactins-a few family of siderophores from Pseudomonas. Biometals, 1993, 6; 93-100.
[55]
Saxena, B., Modi, M., and Modi, V. V. Isolation and characterization of siderophores from Azospirillum lipoferum D-2. J. Gen. Microbiol, 1986, 132; 2219-2224.
[56]
Neilands, J. B. Microbial iron compounds, Ann. Rev. Biochem, 1981, 50; 715.
[57]
Bevivino, A., Tabacchioni, S., Chiarini, L., Carusi, M. V., Del Gallo, M., V. et. al. Phenotypic comparison between rhizosphere and clinical isolates of Burkholderia cepacia. Microbiol, 1994, 140; 1069-1077.
[58]
Shimaila, R., Trevor, C. C., and Bernard R. G. Isolation and characterization of new plant growth-promoting bacterial Endophytes. Appl. Soil Ecology, 2012, 61; 217–224.
[59]
Glick, B. R., Penrose, D. M., and Li, J. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. J. Theor. Biol, 1998, 190; 63-68.
[60]
Esashi, Y. Ethylene and seed germination. In: The plant hormone ethylene (eds.) A. K. Matoo and J. C. Suttle, CRC press, Boca Raton, FL, 1991, pp. 133-157.
[61]
Abdul, L. K., Boshra, A. H., Ali, E., Sajid Ali, Khadija, Al. et al. Indole acetic acid and ACC deaminase fromendophytic bacteria improves the growth of Solanum lycopersicum. Electr. J. Biotechnol, 2016, 21; 58–64.
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