Formulation of Eco-friendly Management Package Against Seedling Disease Caused by Sclerotium rolfsii of Lentil
American Journal of BioScience
Volume 8, Issue 3, May 2020, Pages: 65-72
Received: Mar. 3, 2020; Accepted: Mar. 19, 2020; Published: Jun. 8, 2020
Views 67      Downloads 39
Md. Iqbal Faruk, Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Md. Monirul Islam, Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Firoza Khatun, Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
Article Tools
Follow on us
The experiments were conducted in the fields of Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur during 2014-15, 2015-16 and 2016-17 cropping years to observe the effect of formulated Trichoderma harzianum (Soil amendment with Tricho-compost and seed treatment with T. harzianum spore suspension) and organic soil amendment poultry refuse either singly or in combination with seed treatment with fungicide Provax 200 WP against soil-borne pathogens, Sclerotium rolfsii of lentil causing seedling disease. The partially decomposed poultry refuse was incorporated in the 2 weeks before seed sowing of lentil where Tricho-composts were incorporated in the soil 7 days before seed sowing. Seeds were treated with Trichoderma spore suspension and Provax 200 WP at the time of seed sowing. From this study it was revealed that soil amendment with Tricho-compost or integration poultry refuse with seed treatment by Provax 200 WP performed as the best treatments in reducing seedling mortality and increasing plant growth and yield of lentil which was significantly differed from the other treatments including control. Seed treatment with chemical fungicide provax showed better performance against the disease also seed treatments with Trichoderma spores suspension and soil amendment with poultry refuse which effect at per. Both of them reduced seedling mortality and increased plant growth and yield of lentil.
Trichoderma Harzianum, Sclerotium rolfsii, Lens Culinaris, Lentil, Seedling Disease
To cite this article
Md. Iqbal Faruk, Md. Monirul Islam, Firoza Khatun, Formulation of Eco-friendly Management Package Against Seedling Disease Caused by Sclerotium rolfsii of Lentil, American Journal of BioScience. Vol. 8, No. 3, 2020, pp. 65-72. doi: 10.11648/j.ajbio.20200803.13
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
BBS. 2011. Bangladesh Bureau of Statistics. Ministry of Planning. Dhaka, Bangladesh. 126.
Sattar MA Podder AR, Chandra MC., Rahman M. 1996. The most promising BNF technology for green legume production in Bangladesh. BNF Association, Dhaka, BD. 28, Nov, 1994 pp. 15-20.
Hossain I, Khan MAI, Podder AK. 1999. Seed treatment with Rhizobium in controlling Fusarium oxysporum and Sclerotium rolfsii for biomass and seed production of lentil (Lens cuilinaris M.). Bangladesh J. Environ. Sci. 5: 61-64.
Fakir GA. 1983. Status of research on pulse disease at the BAU, Department of Plant Pathology BAU, Mymensingh.
Begum F. 2003. Integrated control of seedling mortality of lentil caused by Sclerotium rolfsii. MS thesis submitted to the Department of Plant Pathology, Bangladesh Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh.
Ahmed HU. 1985. Disease problems of pulse and oil seed crops in Bangladesh. A paper presented in the 1st National Phytopathology. Conf. BARI Gazipur.
Gerhardson B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20: 338-343.
Larkin RP, Roberts D., Gracia-Garza JA. 1998. Biological control of fungal diseases. In Fungicidal activity, chemical and biological approaches. (pp 141- 191). New York, NY: Wiley.
Kirkegaard JA, Sarwar M, Wong PTW, Mead A, Howe G, Newell M. 2000. Field studies on the biofumigation of take-all by Brassica break crops. Australian Journal of Agricultural Research, 51 (4), 445-456. doi: 10.1071/AR99106.
Ryckeboer J. 2001. Biowaste and yard waste composts: Microbiological and hygienic aspects: Suppressiveness to plant diseases. Katholieke Universiteit Leuven, Faculteit Landbouwkundige en Toegepaste, Biologische Wetenschappen, Laboratorium voor Fytopathologie en Plantenbescherming.
Bailey KL, Lazarovits G. 2003. Suppressing soilborne diseases with residue management and organic amendments. Soil and Tillage Research, 72 (2), 169-180.
Louws FJ, Rivard CL, Kubota C. 2010. Grafting fruiting vegetables to manage soilborne pathogens, foliar pathogens, arthropods and weeds. Scientia Horticulturae, 127 (2), 127-146.
Harman GE. 2011. Multifunctional fungal plant symbionts: new tools to enhance plant growth and Productivity. New Phytologist Commentry, Forum (3): 647-649.
Singh BN, Singh A, Singh SP, Singh HB. 2011. Trichoderma harzianum-mediated reprogramming of oxidative stress response in root apoplast of sunflower enhances defense against Rhizoctonia solani. European Journal of Plant Pathology 131 (1): 121-134.
Benitez T, Rincon AM, Limon MC, Codon AC. 2004. Biocontrol mechanisms of Trichodermastrains, a review article. Intl. Microbiol. 7: 249-260.
Harman GE. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopathology, 96: 190-194.
Shalini KP, Lata Narayan, Kotasthane AS. 2006. Genetic relatedness among Trichoderma isolates inhibiting a pathogenic fungi Rhizoctonia solani, African Journal of Biotechnology, 5 (8): 580-584.
Das BC, Roy SK, Bora LC. 1997. Mass multiplication of Trichoderma species on different media. J. Agril. Sci. Society of North East India. 10 (1): 95-100.
Shamsuzzaman, Islam SMA, Hossain I. 2003. Trichoderma culture and germination of sweet gourd seed. Bangladesh J. Seed Sci. and Tech. 7 (1 and 2): 91-95.
Rettinassababady C, Ramadoss N. 2000. Effect of different substrates on the growth and sporulation of Trichoderma viride native isolates. Agril. Sci. Digest. 20 (3): 150-152.
Cotxarrera L, Trillas-Gay MI, Steinberg C, Alabouvette C. 2002. Use of sewage sludge compost and Trichoderma asperellum isolates to suppress Fusarium wilt of tomato. Soil Biology and Biochemistry, 34, 467–476.
Magid J, Henriksen O, Thorup-Kristensen K, Mueller T. 2001. Disproportionately high N-mineralisation rates from green manures at low temperatures – implications for modelling and management in cool temperate agro-ecosystems. Plant and Soil 228: 73-82.
Conklin AE, Erich MS, Liebman M, Lambert D, Gallandt ER, Halteman WA. 2002. Effects of red clover (Trifolium pratense) green manure and compost soil amendments on wild mustard (Brassica kaber) growth and incidence of disease. Plant and Soil 238: 245-256.
Cavigelli MA, Thien SJ. 2003. Phosphorus bioavailability following incorporation of green manure crops. Soil Science Society American Journal 67: 1186-1194.
Litterick AM, Harrier L, Wallace P, Watson CA, Wood M. 2004. The role of uncomposted materials, composts, manures, and compost extracts in reducing pest and disease incidence and severity in sustainable temperate agricultural and horticultural crop production: A review. Critical Reviews in Plant Sciences 23: 453-479.
Noble R, Coventry E. 2005. Suppression of soil-borne plant diseases with composts: a review. Biocontrol Science and Technology 15: 3-20.
Baxter AP, Rong IH, Roux C, Van der Linde EJ. 1999. Collecting and Preserving Fungi-A Manual for Mycology. Plant Protection Research Institute. Private Bag X134, Pretoria, 0001 South Africa.
Barnett HL, Hunter BB. 1972. Illustrated Genera of Imperfect Fungi. 3rd Ed. Burges Co., Minneapolis, USA.
Booth C. 1971. The Genus Fusarium. Commonwealth Mycology Institute Kew, Survey, England.
Wright RJ., (Ed.) 1998. Agricultural uses of municipal, animal, and industrial byproducts. USDA. Agricultural Research Service, Conservation Research Report, (44). Retrieved from
Mehta CM, Palni U, Franke-Whittle IH, Sharma AK. 2014. Compost: Its role, mechanism and impact on reducing soil-borne plant diseases. Waste Management, 34 (3), 607-22. pmid 24373678.
Ramette A, Frapolli M, Defago G, Moenne-Loccoz Y. 2003. Phylogeny of HCN synthase-encoding hcnBC genes in biocontrol fluorescent pseudomonads and its relationship with host plant species and HCN synthesis ability. Molecular Plant-Microbe Interactions, 16 (6), 525-35. pmid: 12795378.
Berg G, Kurze S, Buchner A, Wellington EM, Smalla K. 2000. Successful strategy for the selection of new strawberry-associated rhizobacteria antagonistic to Verticillium wilt. Canadian Journal of Microbiology, 46 (12), 1128-37. pmid: 11142403.
Mavrodi OV, Walter N, Elateek S, Taylor CG, Okubara PA. 2012. Suppression of Rhizoctonia and Pythium root rot of wheat by new strains of Pseudomonas. Biological Control, 62 (2), 93-102.
Bhattacharjee R, Dey U. 2014. An overview of fungal and bacterial biopesticides to control plant pathogens/diseases. African Journal of Microbiology Research, 8 (17), 1749-1762. doi 10.5897/AJMR2013.6356.
Mukry SN, Ahmad A, Khan SA. 2010. Screening and partial characterization of hemolysins from Bacillus sp.: Strain S128 & S144 are hemolysin B (HBL) producers. Pakistan Journal of Botany, 42 (1), 463-472.
Uzun I. 2004. Use of spent mushroom compost in sustainable fruit production. Journal of Fruit and Ornamental Plant Research. 12: 157-165.
Younis NA. 2005. Mycoparasitism of Trichoderma harzianum and Trichoderma longibrachiatum on Fusarium oxysporum f.sp. phaseoli the causal of bean wilt disease. Bull. Faculty Agric. Cairo Univ. 56: 201-219.
Katan J. 1999. The methyl bromide issue: Problems and potential solutions. Journal of Plant Pathology, 81, 153-159. doi 10.4454/jpp.v81i3.1071.
Omar I, O’neill TM, Rossall S. 2006. Biological control of fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim. Plant Pathology, 55 (1), 92-99. doi: 10.1111/j.1365-3059.2005.01315.x.
Klein E, Katan J, Austerweil M, Gamliel A. 2007. Controlled laboratory system to study soil solarization and organic amendment effects on plant pathogens. Phytopathology, 97 (11), 1476-1483. pmid: 18943518.
Gamliel A, Katan J. 2009. Control of plant disease through soil solarization. In D. Walters (Ed.), Disease Control in Crops. (pp 196-220). Edinburgh, UK: Wiley-Blackwell Publishing Ltd.
Slusarski C, Ciesielska J, Malusa E, Meszka B, Sobiczewski P. 2012. Metam sodium, metam potassium and dazomet. In Sustainable use of chemical fumigants for the control of soil-borne pathogens in the horticultural sector. Skierniewice, Poland: Research Insititute of Horticulture.
Deshmukh PP, Raut JG. 1992. Antagonism by Trichoderma spp. on five plant pathogenic fungi. New Agriculturist. 3 (2): 127-130.
Xu T, Zhong JP, Li DB. 1993. Antagonism of T. harzianum T82 and Trichoderma species NF9 against soil and seed borne pathogens. Acta. Phytopathol. Ca. Scinica, 23 (1) 63-67.
Askew DJ, Laing MD. 1994. The in-vitro screening of Trichoderma isolates for antagonism to Rhizoctonia solani and an evaluation of different environmental sites of Trichoderma as sources of aggressive strains. Plant and Soil 159 (2): 227-281.
Hossain I, Shamsuzzaman SM. 2003. Developing Trichoderma based bio-fungicide using agro-waste. BAU Res. Prog. 14: 49-50.
Hossain I., Naznin M. H. A. 2005. BAU biofungicide in controlling seedling disease of some summer vegetables. BAU Res. Progr. 15: 32-35.
Shaban WI, El-Bramawy MA. 2011. Impact of dual inoculation with Rhizobium and Trichoderma on damping off, root rot diseases and plant growth parameters of some legumes field crop under greenhouse conditions. Int. Res. J. Agric. Sci. Soil Sci. 1: 98-108.
Ristaino JB. 2002. Effect of synthetic and organic soil fertility amendments on southern blight, soil microbial communities, and yield of processing tomatoes. Phytopathology 92: 181-189.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186