Structure and Physicochemical Properties of Nitrogenated Derivatives of Cellulose
American Journal of BioScience
Volume 2, Issue 6-1, November 2014, Pages: 6-12
Received: Jun. 28, 2014; Accepted: Jul. 10, 2014; Published: Jul. 13, 2014
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Michael Ioelovich, Designer Energy Ltd, Rehovot, Israel
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Nitrogenated derivatives of cellulose, and namely chitin and chitosan, are of commercial interest because of their antibacterial activity, biocompatibility, biodegradability and sorption ability. Structure and some physicochemical properties of these derivatives have been studied by methods of wide-angle X-ray scattering (WAXS), microcalorimetry and sorption. To determine the crystallinity degree of the samples, an improved WAXS method was used based on the calculation of the relationship between integrated intensities of X-ray diffraction from crystalline and amorphous domains. The calculations revealed that the actual degree of crystallinity of initial chitin was 0.72, and of initial chitosan 0.57. After ball-grinding for a short time, the decrease in the crystallinity degree was observed, whereas the prolonged grinding resulted in complete amorphization of the samples. The wetting enthalpy and sorption ability of the samples were inversely proportional to degree of crystallinity. Reduction of the crystallinity degree of the samples promoted increasing of the wetting enthalpy and water sorption. This evidences that mechanism of interaction between the nitrogenated polysaccharides and water is absorption of the water molecules into amorphous domains of the hydrophilic polymers. The crystallinity degree values calculated from results of water vapor sorption and wetting enthalpy were close to the crystallinity degree of the samples obtained by the WAXS method.
Chitin, Chitosan, Crystallinity Degree, Wetting Enthalpy, Sorption Ability
To cite this article
Michael Ioelovich, Structure and Physicochemical Properties of Nitrogenated Derivatives of Cellulose, American Journal of BioScience. Special Issue:Chemical Biology. Vol. 2, No. 6-1, 2014, pp. 6-12. doi: 10.11648/j.ajbio.s.2014020601.12
Rinaudo M. “Chitin and chitosan: properties and applications,” Progr. Polym. Sci., vol. 31, pp. 603-632, 2006.
Aranaz I., Mengibar M., Harris R., Panos I., Miralles B., Acosta N., Galed G., and Heras A. “Functional characterization of chitin and chitosan,” Current Chem. Biol., vol. 3, pp. 203-230, 2009.
Roberts G. Chitin Chemistry. London: MacMillan, 1998.
Struszczyk M.H. “Applications of chitosan,” Polymery, vol. 47, pp. 396-403, 2002
Muzzarelly R.A.A., and Muzzarelly C. “Chitosan chemistry: relevance to the biomedical sciences,” Adv. Polym. Sci., vol. 186, pp.151-209, 2005.
Diaz-Rojas E.I., Arguelles-Monal W.M., Higuera-Ciapara I., Hernandes J., Lizardi-Mendoza J., and Goycoolea F.M. “Determination of chitin and protein content during the isolation of chitin from shrimp waste,” Macromol. Biosci., vol. 6, pp. 340-347, 2006.
Dutta P.K, Dutta J., and. Tripathi V.S. “Chitin and chitosan: chemistry, properties and applications,” J. Sci. Ind. Res., vol. 63, pp. 20-31, 2004.
Blackwell J. Biopolymers. New York: Academic Press, 1973.
Watthanaphanit A., and Rujiravanit R. “Structural organization and biological activity of chitin nanofibrils,” in Handbook of Carbohydrate Polymers: Development, Properties and Applications. New York: Nova Publishers, 2010.
Minke R., and Blackwell J. “The structure of chitin,” J. Mil. Biol., vol. 120, pp. 167-181, 1978.
Rudall K.M., and Kenchington W. “The α-chitin system,” Biol. Rev., vol. 40, pp. 597-639, 1973.
Gardner K.H., and Blackwell J. “Refinement of the structure of β-chitin,” Biopolymers, vol. 14, pp. 1581-1595, 1975.
Saito Y., Okano T., Gaill F., Chanzy H., and Putaux J.-L. “Structural data on the intra-crystalline swelling of β-chitin,“ Int. J. Biol. Macromol., vol. 28, pp. 81-88, 2000.
Saito Y., Putaux J.-L., Okano T., Gaill F., and Chanzy H. “Structural aspects of the swelling of β-chitin in HCl and its conversion into α-chitin,” Macromolecules, vol. 30, pp. 3867-387, 1997.
Cartier N., Domand A., and Chanzy H. “Single crystals of chitosan,” Int. J. Biol. Macromol., vol. 12, pp. 289-294, 1990.
Zhang Y., Xue C., Xue Y., Gao R., and Zhang X. “Determination of the degree of deacetylation of chitin and chitosan by X-ray powder diffraction,” Carbohydr Res., vol. 340, pp. 1914-1917, 2005.
Kumirska J., Czerwicka M., Kaczyński Z., Bychowska A., Brzozowski K., Thöming J., and Stepnowski P. “Application of spectroscopic methods for structural analysis of chitin and chitosan,” Marine Drugs, vol. 8, pp.1567–1636, 2010.
Focher B., Beltranme P.L., Naggi A., and Torri G. “Alkaline N-deacetylation of chitin enhanced by flash treatments: reaction kinetics and structure modifications,” Carbohydr Polym., vol. 12, pp. 405–418, 1990.
Cruz M., Ravagnani S.P., Brogna F., Campana S.P., Triviño G.C., Lisboa A., and Mei L. “Evaluation of the diffusion coefficient for controlled release of oxytetracycline from alginate/chitosan/poly(ethylene glycol) microbeads in simulated gastrointestinal environments,” Biotechn. Appl. Biochem., vol. 40, pp. 243–253, 2004.
Yuan Y., Chesnutt B.M., Haggard W.O., and Bumgardner J.D. “Deacetylation of chitosan: material characterization and in vitro evaluation via albumin adsorption and preosteoblastic cell cultures,” Materials, vol. 4, pp. 1399-1416, 2011.
Struszczyk H. “Microcrystalline chitosan: preparation and properties of microcrystalline chitosan,” J. Appl. Polym. Sci., vol. 33, pp. 177–189, 1987.
Abdou E.S., Nagy K.S.A., and Elsabee M.Z. “Extraction and characterization of chitin and chitosan from local sources,” Biores. Technol., vol. 99, pp. 1359–1367, 2008.
Fan Y., Saito T., and Isogai A. “Chitin nanocrystals prepared by TEMPO-mediated oxidation of alpha chitin,” Biomacromolecules, vol. 9, pp. 192-198, 2008.
Fan Y., and Saito T. “Nano-fibrillation of chitins by TEMPO-mediated oxidation or protonation of amino groups,” Funct. Mater., vol. 29, pp.19-24, 2009.
Chebotok E.N., Novikov V.Y., and Konovalova I.N. “Effect of crystallinity of chitin and chitosan on kinetics of alkaline deacetylation,” J. Appl. Chem., vol. 80, pp. 1724-1729, 2007.
Vihoreva G.A., Gorbacheva I.N., and Galbraich L.S. “Chitin and chitosan,” Chem. Fibers, vol. 5, pp. 36-45, 1994.
Maryin A.P., Feophylova E.P., and Genin Y.V. “Effect of crystallinity on sorption and thermal properties of chitosan,” High-Mol. Polym. Comp., vol. 24b, pp. 658-662, 1982.
Ioelovich M. Cellulose: Nanostructured Natural Polymer. Saarbrucken: Lambert Academic Publishing, 2014.
Mucha M., and Balcerzak J. “Thermal effect accompanying water adsorption by chitosan with different deacetylation degrees,” Polish Chitin Soc., vol. 12, pp. 49-55, 2007.
Prusov A.N., Prusova S.M., Radugin M.V., and Zakharov A.G. “Interrelation between the crystallinity of polysaccharides and water absorption,” Russian Journal Phys. Chem., vol. 88, pp. 813-818, 2014.
Ioelovich M. “Heat effects of interaction between cellulose and various polar liquids,” SITA, vol. 13, pp. 35-44, 2011.
Ioelovich M., and Leykin A. “Study of sorption properties of cellulose and its derivatives,” Bioresources, vol. 6, pp.178-195, 2011.
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