Hepatoprotective Effect of Sesame Oil against Lead Induced Liver Damage in Albino Mice: Histological and Biochemical Studies
The liver performs many vital functions to eliminate toxins and harmful substances from the body. Hepatotoxic agents can react with the basic cellular components and consequently induce almost all types of liver lesions. The aim of this study was to investigate the possible hepatoprotective role of sesame oil against lead acetate induced hepatotoxicity in albino mice from the histological and biochemical aspects. In this study, thirty two adult male albino mice were used for this study and divided into four groups. The first group was control group, the 2nd was the sesame oil group orally received sesame oil (5 ml/kg body wt.), the 3rd group was the experimental and received lead acetate (500 mg /kg diet), the 4th one co-administered lead acetate (500 mg/kg diet) with sesame oil (5 ml/kg body wt.) daily for 30 days. The livers were dissected out, weighted and specimens were taken and processed for light microscopic examinations. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, serum total proteins and albumin. Results indicate that, in lead treated animals, there were severe structural damage in the liver. The hepatocytes appeared irregularly arranged with disorganization of hepatic architecture. The hepatocytes appeared large with light and foamy cytoplasm filled with numerous vacuole-like spaces. The nuclei appeared with pyknotic nuclei. The central vein appeared dilated and congested with massive hemorrhage extending to the nearby cells. Also, there were focal degenerative and necrotic changes along with inflammatory cell infiltration. Decrease in body weight and increase in liver weight were observed. Biochemically, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ- glutamyltransferase activities were increased and serum total proteins and albumin were decreased. Co-administration of sesame oil significantly improved the structural changes in the liver and also the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities were significantly declined and serum total proteins and albumin were elevated. Conclusion: It can be concluded that, the lead had adverse effects on the liver. Sesame oil showed effective hepatoprotective action against lead acetate induced hepatotoxicity in albino mice. So, the populations of high risk to lead should be advised to take sesame oil.
Azab El-Saied Azab,
Hepatoprotective Effect of Sesame Oil against Lead Induced Liver Damage in Albino Mice: Histological and Biochemical Studies, American Journal of BioScience. Special Issue:Natural Products: Health and Disease.
Vol. 2, No. 6-2,
2014, pp. 1-11.
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