Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis

Mohamed Elmogy; Azza M. Elgendy; Makio Takeda

doi:doi:10.11648/j.ajbio.20140203.15

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Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis

Mohamed Elmogy, Azza M. Elgendy, Makio Takeda

Received: 12 April 2014 Accepted: 28 April 2014 Published: 30 May 2014

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Abstract

In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.

DOI	10.11648/j.ajbio.20140203.15
Published in	American Journal of BioScience (Volume 2, Issue 3, May 2014)
Page(s)	106-110
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

GTP/ATP Binding, GTPase, Vitellogenesis, Endocytosis, American Cockroach

References

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APA Style

Mohamed Elmogy, Azza M. Elgendy, Makio Takeda. (2014). Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. American Journal of BioScience, 2(3), 106-110. https://doi.org/10.11648/j.ajbio.20140203.15

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ACS Style

Mohamed Elmogy; Azza M. Elgendy; Makio Takeda. Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. Am. J. BioScience 2014, 2(3), 106-110. doi: 10.11648/j.ajbio.20140203.15

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AMA Style

Mohamed Elmogy, Azza M. Elgendy, Makio Takeda. Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. Am J BioScience. 2014;2(3):106-110. doi: 10.11648/j.ajbio.20140203.15

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@article{10.11648/j.ajbio.20140203.15,
  author = {Mohamed Elmogy and Azza M. Elgendy and Makio Takeda},
  title = {Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {3},
  pages = {106-110},
  doi = {10.11648/j.ajbio.20140203.15},
  url = {https://doi.org/10.11648/j.ajbio.20140203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140203.15},
  abstract = {In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.},
 year = {2014}
}

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TY  - JOUR
T1  - Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis
AU  - Mohamed Elmogy
AU  - Azza M. Elgendy
AU  - Makio Takeda
Y1  - 2014/05/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140203.15
DO  - 10.11648/j.ajbio.20140203.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 106
EP  - 110
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140203.15
AB  - In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.
VL  - 2
IS  - 3
ER  -

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Author Information

Mohamed Elmogy

Department of Entomology, Faculty of Science, Biotechnology program, Cairo University, Giza, Egypt; Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia
Azza M. Elgendy

Department of Entomology, Faculty of Science, Biotechnology program, Cairo University, Giza, Egypt
Makio Takeda

Insect Science Laboratory, Graduate School of Agricultural, Kobe University, Kobe, Japan

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Mohamed Elmogy, Azza M. Elgendy, Makio Takeda. (2014). Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. American Journal of BioScience, 2(3), 106-110. https://doi.org/10.11648/j.ajbio.20140203.15

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ACS Style

Mohamed Elmogy; Azza M. Elgendy; Makio Takeda. Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. Am. J. BioScience 2014, 2(3), 106-110. doi: 10.11648/j.ajbio.20140203.15

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AMA Style

Mohamed Elmogy, Azza M. Elgendy, Makio Takeda. Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis. Am J BioScience. 2014;2(3):106-110. doi: 10.11648/j.ajbio.20140203.15

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@article{10.11648/j.ajbio.20140203.15,
  author = {Mohamed Elmogy and Azza M. Elgendy and Makio Takeda},
  title = {Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis},
  journal = {American Journal of BioScience},
  volume = {2},
  number = {3},
  pages = {106-110},
  doi = {10.11648/j.ajbio.20140203.15},
  url = {https://doi.org/10.11648/j.ajbio.20140203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20140203.15},
  abstract = {In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.},
 year = {2014}
}

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TY  - JOUR
T1  - Detection of Small GTP Binding Proteins Showing GTPase and GTP/ATP Binding Activities in the Ovary of the American Cockroach, Periplaneta Americana, during Oogenesis
AU  - Mohamed Elmogy
AU  - Azza M. Elgendy
AU  - Makio Takeda
Y1  - 2014/05/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbio.20140203.15
DO  - 10.11648/j.ajbio.20140203.15
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 106
EP  - 110
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20140203.15
AB  - In the present study, the small GTP binding proteins involved in the regulatory mechanism of vitellogenin (Vg) endocytotic vesicles trafficking were detected, for the first time, in ovaries of the most basal hemimetabolus insect, Periplaneta americana during oogenesis. The ovarian GTPase activities were peaked during previtellogenic and early vitellogenic periods. Such activity coincides with vitellogenin receptors (VgRs) and clathrin early expression during these developmental periods, suggesting the importance of GTPases not only in the process of vesicle formation and fusion but also in the process of early fluid phase endocytosis. Two small peaks of activities were monitored during the late vitellogenic period (days 8 and 10), suggesting a possible role of GTPases in VgRs and clathrin recycling process. The [α32P]-GTP binding assay analysis in different tissues revealed the presence of small GTP binding proteins of molecular weights 25, 23 and 21 kDa in ovaries and head. However, a single binding signal band of 21 and 25 kDa was each detected in the fat bodies and muscles, respectively. No binding was detected in the midgut and Malpighian tubules. However, the 23 kDa protein detected was suggested as a probable cytosolic form of the 25 kDa protein. The competition assay results indicated that the small ovarian GTP binding proteins could also bind ATP, suggesting that like GTP, ATP is a regulatory nucleotide for the ovarian small proteins detected during oogenesis. The present study will pave the way for more understanding of the mechanisms that regulate Vg transport machinery in hemimetabolous insects.
VL  - 2
IS  - 3
ER  -

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