American Journal of BioScience

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Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat

Received: 01 February 2016    Accepted: 18 March 2016    Published: 03 May 2016
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Abstract

Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.

DOI 10.11648/j.ajbio.20160402.12
Published in American Journal of BioScience (Volume 4, Issue 2, March 2016)
Page(s) 16-19
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Methodology, Traditional Cure, Oral Glucose, Wistar Rat

References
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[3] Bauck. Basic anatomy, physiology, husbandry and clinical techniques. Part I. In Hillyer, Quesenberry. Ferrets, rabbits and roddents. Clin med and surg, saunders, 2004; 26, 291-297.
[4] Debuigue G. Larousse des plantes qui guérissent. Librairie Larousse, 2010; p. 5-6.
[5] Keita A, Mariko E, Haidara TK. Etude de l'activité hypoglycémiante des feuilles de Sclerocarya birrea (A. Rich) Hochst. (Anacardiaceae) Pharm. Méd. Trad. Afr. 1998, 10: 16-25.
[6] Kerharo J, Adam JC. La pharmacopée sénégalaise traditionnelle: plantes médicinales et tropicales. Paris, Ed Vigot et frères, 1974; 433-35.
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[8] Lokrou A. Guide de prise en charge des diabétiques. Collection santé, Editions Universitaires de Côte d’Ivoire, 2008; 212p.
[9] Mohamed A, Tanko Y, Okasha MA. Effects of aqueous leaves extract of Ocimum gratissimum on blood glucose levels of streptozocin-induced diabetic Wistar rats. Afr J Biotechnolog, 2007; 6, 18: 2087-90.
[10] Monteomo GF, Tehoua L, Ouattara H et al. Comparative antihyperglycemic activity of Sclerocarya birrea, Khaya senegalensis, Heliotropium indicum and Ocimum gratissimum to rats Wistar. Am J Biosciences, 2014; 2, 2: 60-63.
[11] Monteomo GF. Valorisation de la pharmacopée africaine: étude de l’activité antihyperglycémique d’une préparation traditionnelle améliorée composée de sclerocarya birrea (Anacardiacée), Khaya senegalensis (Meliacée), Heliotropium indicum (Borraginacée) et Ocimum gratissimum (Lamiacée). Doctorat des Universités, Option, Biologie Humaine, Université FHB-Abidjan, UFR-SM, 2015; N° 26, 172p.
[12] Nemlin J, Brunel JF. Travaux pratiques de matière médicale, 3ème année, Paris, Masson, 1995-1996: 39-43.
[13] Ojewole JAO. Laboratory evaluation of the hypoglycemic effect of anarcadium occidentale Linn. (Anarcadiaceae) stem-bark extracts in rats. Methods and findings in experimental and clinical, 2003; 25, 3: 199-204.
[14] Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychnos henningsii Gild in streptozotocin-nicotinamide types 2 diabetic rats. J of Pharmaceutical Research, 2012; 11, 1: 221-228.
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[16] Zhao VW, James MU, Schraw TD. Panic-Attac: A Mouse model for Inducible and Reversible β-Cell Ablation. Diab. 2008; 57, 8: 2137-2148.
Author Information
  • Endocrinology and Diabetology Service, CHU-Yopougon-Abidjan, Abidjan, C?te d’Ivoire

  • Laboratory of Animal Physiology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, C?te d’Ivoire

  • Laboratory of Embryol-Cytol-Cytogen UFR-Med. Sciences-Univ F Houphouēt-Boigny, Abidjan, C?te d’Ivoire

  • Laboratory of Animal Biology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, C?te d’Ivoire

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    Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. (2016). Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. American Journal of BioScience, 4(2), 16-19. https://doi.org/10.11648/j.ajbio.20160402.12

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    ACS Style

    Monteomo Gnate François; Koffi Dago Pierre; Atto Virginie; Deh Zhou Patricia; Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am. J. BioScience 2016, 4(2), 16-19. doi: 10.11648/j.ajbio.20160402.12

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    AMA Style

    Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am J BioScience. 2016;4(2):16-19. doi: 10.11648/j.ajbio.20160402.12

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  • @article{10.11648/j.ajbio.20160402.12,
      author = {Monteomo Gnate François and Koffi Dago Pierre and Atto Virginie and Deh Zhou Patricia and Yapi Ahoua},
      title = {Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat},
      journal = {American Journal of BioScience},
      volume = {4},
      number = {2},
      pages = {16-19},
      doi = {10.11648/j.ajbio.20160402.12},
      url = {https://doi.org/10.11648/j.ajbio.20160402.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbio.20160402.12},
      abstract = {Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.},
     year = {2016}
    }
    

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    AU  - Monteomo Gnate François
    AU  - Koffi Dago Pierre
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    AU  - Deh Zhou Patricia
    AU  - Yapi Ahoua
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    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
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    AB  - Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.
    VL  - 4
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