Exponential Growth and Solvents-Production of <i>Clostridium acetobutylicum</i> ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources

Elizabeth Omolola Oladapo; Enimie Endurance Oaikhena; Mohammed Sani Abdulsalami

doi:doi:10.11648/j.ajbio.20170504.12

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Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources

Elizabeth Omolola Oladapo, Enimie Endurance Oaikhena, Mohammed Sani Abdulsalami

Published in American Journal of BioScience (Volume 5, Issue 4)

Received: 26 March 2017 Accepted: 19 April 2017 Published: 19 June 2017

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Abstract

Clostridium acetobutylicum ATCC 824 is a solventogenic, obligate anaerobic bacterium that can grow on various types of carbohydrates and are capable of producing spores. In the present study, Clostridium acetobutylicum was successfully grown on TYA medium (tryptone, yeast, acetate medium) containing two different carbon sources, glucose and sucrose coupled with the production of acids (butyric and acetic acid) and solvents, ABE (acetone, butanol and ethanol). An investigation was undertaken to determine the impact of the two types of carbon sources on the solvent production and growth of Clostridium acetobutylicum. HPLC and GC analysis revealed the amount of acids and solvents produced respectively, as well as the amount of unutilized sugars. The amount of combined ABE produced on glucose (0.19g/l A, 0.39g/l B, 0.06g/l E) was higher than on sucrose as carbon source (0.15g/l A, 0.30g/l B, 0.03g/l E). The colony forming units of Clostridium acetobutylicum grown on glucose (4.70 x 10⁵ units/ml) was higher than on sucrose (0.1 x 10⁵) as judged by dilution spread plating on agar. Hence, Glucose was confirmed as the carbon source characterized by the best performance for solvents production and growth of the bacterium. The whole production process on both glucose and sucrose was observed to mainly influence the production of butanol with the concentration of 0.39g/l and 0.30g/l respectively, over the production of other solvents. Higher amount of solvents was produced at lower pH in both cultures with the different carbon sources. Wet-mounts, gram stain and endospore stain were used to determine the motility, type and sporulation of Clostridium acetobutylicum respectively. Acidogenic phase which seems to couple with the growth of vegetative cells, results into production of acetic and butyric acids. Solventogenic phase commences with a drop in pH and is accompanied by the onset of sporulation.

Published in	American Journal of BioScience (Volume 5, Issue 4)
DOI	10.11648/j.ajbio.20170504.12
Page(s)	64-69
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Clostridium acetobutylicum ATCC 824, Sucrose, Glucose, ABE

References

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[5]	Sandoval, N., Venkataramanan, K., Groth, T. and Papoutsakis, E. (2015). Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth. Biotechnology for Biofuels. 8: 1.
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Elizabeth Omolola Oladapo, Enimie Endurance Oaikhena, Mohammed Sani Abdulsalami. (2017). Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. American Journal of BioScience, 5(4), 64-69. https://doi.org/10.11648/j.ajbio.20170504.12

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ACS Style

Elizabeth Omolola Oladapo; Enimie Endurance Oaikhena; Mohammed Sani Abdulsalami. Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. Am. J. BioScience 2017, 5(4), 64-69. doi: 10.11648/j.ajbio.20170504.12

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AMA Style

Elizabeth Omolola Oladapo, Enimie Endurance Oaikhena, Mohammed Sani Abdulsalami. Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. Am J BioScience. 2017;5(4):64-69. doi: 10.11648/j.ajbio.20170504.12

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@article{10.11648/j.ajbio.20170504.12,
  author = {Elizabeth Omolola Oladapo and Enimie Endurance Oaikhena and Mohammed Sani Abdulsalami},
  title = {Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources},
  journal = {American Journal of BioScience},
  volume = {5},
  number = {4},
  pages = {64-69},
  doi = {10.11648/j.ajbio.20170504.12},
  url = {https://doi.org/10.11648/j.ajbio.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20170504.12},
  abstract = {Clostridium acetobutylicum ATCC 824 is a solventogenic, obligate anaerobic bacterium that can grow on various types of carbohydrates and are capable of producing spores. In the present study, Clostridium acetobutylicum was successfully grown on TYA medium (tryptone, yeast, acetate medium) containing two different carbon sources, glucose and sucrose coupled with the production of acids (butyric and acetic acid) and solvents, ABE (acetone, butanol and ethanol). An investigation was undertaken to determine the impact of the two types of carbon sources on the solvent production and growth of Clostridium acetobutylicum. HPLC and GC analysis revealed the amount of acids and solvents produced respectively, as well as the amount of unutilized sugars. The amount of combined ABE produced on glucose (0.19g/l A, 0.39g/l B, 0.06g/l E) was higher than on sucrose as carbon source (0.15g/l A, 0.30g/l B, 0.03g/l E). The colony forming units of Clostridium acetobutylicum grown on glucose (4.70 x 105 units/ml) was higher than on sucrose (0.1 x 105) as judged by dilution spread plating on agar. Hence, Glucose was confirmed as the carbon source characterized by the best performance for solvents production and growth of the bacterium. The whole production process on both glucose and sucrose was observed to mainly influence the production of butanol with the concentration of 0.39g/l and 0.30g/l respectively, over the production of other solvents. Higher amount of solvents was produced at lower pH in both cultures with the different carbon sources. Wet-mounts, gram stain and endospore stain were used to determine the motility, type and sporulation of Clostridium acetobutylicum respectively. Acidogenic phase which seems to couple with the growth of vegetative cells, results into production of acetic and butyric acids. Solventogenic phase commences with a drop in pH and is accompanied by the onset of sporulation.},
 year = {2017}
}

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TY  - JOUR
T1  - Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources
AU  - Elizabeth Omolola Oladapo
AU  - Enimie Endurance Oaikhena
AU  - Mohammed Sani Abdulsalami
Y1  - 2017/06/19
PY  - 2017
N1  - https://doi.org/10.11648/j.ajbio.20170504.12
DO  - 10.11648/j.ajbio.20170504.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 64
EP  - 69
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20170504.12
AB  - Clostridium acetobutylicum ATCC 824 is a solventogenic, obligate anaerobic bacterium that can grow on various types of carbohydrates and are capable of producing spores. In the present study, Clostridium acetobutylicum was successfully grown on TYA medium (tryptone, yeast, acetate medium) containing two different carbon sources, glucose and sucrose coupled with the production of acids (butyric and acetic acid) and solvents, ABE (acetone, butanol and ethanol). An investigation was undertaken to determine the impact of the two types of carbon sources on the solvent production and growth of Clostridium acetobutylicum. HPLC and GC analysis revealed the amount of acids and solvents produced respectively, as well as the amount of unutilized sugars. The amount of combined ABE produced on glucose (0.19g/l A, 0.39g/l B, 0.06g/l E) was higher than on sucrose as carbon source (0.15g/l A, 0.30g/l B, 0.03g/l E). The colony forming units of Clostridium acetobutylicum grown on glucose (4.70 x 105 units/ml) was higher than on sucrose (0.1 x 105) as judged by dilution spread plating on agar. Hence, Glucose was confirmed as the carbon source characterized by the best performance for solvents production and growth of the bacterium. The whole production process on both glucose and sucrose was observed to mainly influence the production of butanol with the concentration of 0.39g/l and 0.30g/l respectively, over the production of other solvents. Higher amount of solvents was produced at lower pH in both cultures with the different carbon sources. Wet-mounts, gram stain and endospore stain were used to determine the motility, type and sporulation of Clostridium acetobutylicum respectively. Acidogenic phase which seems to couple with the growth of vegetative cells, results into production of acetic and butyric acids. Solventogenic phase commences with a drop in pH and is accompanied by the onset of sporulation.
VL  - 5
IS  - 4
ER  -

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Author Information

Elizabeth Omolola Oladapo

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Enimie Endurance Oaikhena

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Mohammed Sani Abdulsalami

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria

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APA Style

Elizabeth Omolola Oladapo, Enimie Endurance Oaikhena, Mohammed Sani Abdulsalami. (2017). Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. American Journal of BioScience, 5(4), 64-69. https://doi.org/10.11648/j.ajbio.20170504.12

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ACS Style

Elizabeth Omolola Oladapo; Enimie Endurance Oaikhena; Mohammed Sani Abdulsalami. Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. Am. J. BioScience 2017, 5(4), 64-69. doi: 10.11648/j.ajbio.20170504.12

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AMA Style

Elizabeth Omolola Oladapo, Enimie Endurance Oaikhena, Mohammed Sani Abdulsalami. Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources. Am J BioScience. 2017;5(4):64-69. doi: 10.11648/j.ajbio.20170504.12

Copy | Download

@article{10.11648/j.ajbio.20170504.12,
  author = {Elizabeth Omolola Oladapo and Enimie Endurance Oaikhena and Mohammed Sani Abdulsalami},
  title = {Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources},
  journal = {American Journal of BioScience},
  volume = {5},
  number = {4},
  pages = {64-69},
  doi = {10.11648/j.ajbio.20170504.12},
  url = {https://doi.org/10.11648/j.ajbio.20170504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20170504.12},
  abstract = {Clostridium acetobutylicum ATCC 824 is a solventogenic, obligate anaerobic bacterium that can grow on various types of carbohydrates and are capable of producing spores. In the present study, Clostridium acetobutylicum was successfully grown on TYA medium (tryptone, yeast, acetate medium) containing two different carbon sources, glucose and sucrose coupled with the production of acids (butyric and acetic acid) and solvents, ABE (acetone, butanol and ethanol). An investigation was undertaken to determine the impact of the two types of carbon sources on the solvent production and growth of Clostridium acetobutylicum. HPLC and GC analysis revealed the amount of acids and solvents produced respectively, as well as the amount of unutilized sugars. The amount of combined ABE produced on glucose (0.19g/l A, 0.39g/l B, 0.06g/l E) was higher than on sucrose as carbon source (0.15g/l A, 0.30g/l B, 0.03g/l E). The colony forming units of Clostridium acetobutylicum grown on glucose (4.70 x 105 units/ml) was higher than on sucrose (0.1 x 105) as judged by dilution spread plating on agar. Hence, Glucose was confirmed as the carbon source characterized by the best performance for solvents production and growth of the bacterium. The whole production process on both glucose and sucrose was observed to mainly influence the production of butanol with the concentration of 0.39g/l and 0.30g/l respectively, over the production of other solvents. Higher amount of solvents was produced at lower pH in both cultures with the different carbon sources. Wet-mounts, gram stain and endospore stain were used to determine the motility, type and sporulation of Clostridium acetobutylicum respectively. Acidogenic phase which seems to couple with the growth of vegetative cells, results into production of acetic and butyric acids. Solventogenic phase commences with a drop in pH and is accompanied by the onset of sporulation.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Exponential Growth and Solvents-Production of Clostridium acetobutylicum ATCC 824 on TYA Media Containing Sucrose and Glucose as Different Sole Carbon Sources
AU  - Elizabeth Omolola Oladapo
AU  - Enimie Endurance Oaikhena
AU  - Mohammed Sani Abdulsalami
Y1  - 2017/06/19
PY  - 2017
N1  - https://doi.org/10.11648/j.ajbio.20170504.12
DO  - 10.11648/j.ajbio.20170504.12
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 64
EP  - 69
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20170504.12
AB  - Clostridium acetobutylicum ATCC 824 is a solventogenic, obligate anaerobic bacterium that can grow on various types of carbohydrates and are capable of producing spores. In the present study, Clostridium acetobutylicum was successfully grown on TYA medium (tryptone, yeast, acetate medium) containing two different carbon sources, glucose and sucrose coupled with the production of acids (butyric and acetic acid) and solvents, ABE (acetone, butanol and ethanol). An investigation was undertaken to determine the impact of the two types of carbon sources on the solvent production and growth of Clostridium acetobutylicum. HPLC and GC analysis revealed the amount of acids and solvents produced respectively, as well as the amount of unutilized sugars. The amount of combined ABE produced on glucose (0.19g/l A, 0.39g/l B, 0.06g/l E) was higher than on sucrose as carbon source (0.15g/l A, 0.30g/l B, 0.03g/l E). The colony forming units of Clostridium acetobutylicum grown on glucose (4.70 x 105 units/ml) was higher than on sucrose (0.1 x 105) as judged by dilution spread plating on agar. Hence, Glucose was confirmed as the carbon source characterized by the best performance for solvents production and growth of the bacterium. The whole production process on both glucose and sucrose was observed to mainly influence the production of butanol with the concentration of 0.39g/l and 0.30g/l respectively, over the production of other solvents. Higher amount of solvents was produced at lower pH in both cultures with the different carbon sources. Wet-mounts, gram stain and endospore stain were used to determine the motility, type and sporulation of Clostridium acetobutylicum respectively. Acidogenic phase which seems to couple with the growth of vegetative cells, results into production of acetic and butyric acids. Solventogenic phase commences with a drop in pH and is accompanied by the onset of sporulation.
VL  - 5
IS  - 4
ER  -

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