Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide
American Journal of BioScience
Volume 7, Issue 1, January 2019, Pages: 25-30
Received: Jun. 13, 2018; Accepted: Jul. 7, 2018; Published: Apr. 13, 2019
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Odo Joel Inya, Department of Fisheries and Aquaculture, University of Agriculture, Makurdi, Nigeria
Amuta Elizabeth, Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Umeh Ebele, Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Azua Emmanuel, Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Odo Edna, Department of Fisheries and Aquaculture, University of Agriculture, Makurdi, Nigeria
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The study was carried out to determine the effects of sub lethal concentrations of vestaline® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P< 0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P<0.05) in amount of HCT, Hb and RBC with increase in concentration an indication of Anaemia. While WBC increased significantly (P<0.05) with increase in concentration. All were dose dependant. The results of Serum Biochemistry showed significant difference (P<0.05) in all the parameters determined. There was significant (P<0.05) decrease in Total protein and Albumin while Cholesterol, Alanine aminoTransferase, Aspartate aminoTransferase and Creatine all increased significantly (P<0.05) with increase in concentration. Vestaline® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.
Clarias gariepinus, Vestalin® (Pendimethalin), Hematology
To cite this article
Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna, Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide, American Journal of BioScience. Vol. 7, No. 1, 2019, pp. 25-30. doi: 10.11648/j.ajbio.20190701.14
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Adedeji O. B., Adeyemo O. K., Agbede S. A. (2009). Effects of diazinon on blood parameters in the African catfish (Clarias gariepinus). African J. Biotech. 6: 3940-3946.
Adeyemo, O. K. (2005). Haemological and histopathological effects of cassava mill Effluent in Clarias gariepinus. African Journal of Biomedicine Research. 8:179-183.
Adhikari, S., Sarkar, B., Chatterjee, A., Mahapatra, CT. and Ayyappan, S. (2004). Effects of cypermethrin and carbofuran on certain haematological parameters and prediction of their recovery in a freshwater teleost, Labeorohita (Hamilton). Ecotoxicol Environ Saf, 58 (2): 220-226.
Aguigwo JN (1998). Studies on acute toxicity of Cassava leaf extracts on African catfish Clarias angullaris. J. Aquat. Sci. 13: 29-32.
Ahmed SM, Hussein SY, El-Nasser MA. (1996). Comparative studies on the effect of herbicide atrazine on freshwater fish Oreochromis niloticus and Chrysichthye auratus at Assuit. Egypt Bull Envion Contam Toxicol 1996; 57: 503–510.
Annune P A, Ebele S O, Olademeji A A (1994). Acute toxicity of cadmium to juveniles of Clarias gariepinus (Teugels) and Oreochromis niloticus (Trewawas). J. Environ. Sci. Health. A29: 1357-1365.
Awachie JBE, Ezenwaji HMG (1998). The importance of Clarias species in the fishery development of Anambra river basin, Nigeria. CIFA
Ayoola, S. O. (2008). Toxicity of Glyphosate Herbicide on Nile Tilapia (Oreochromis niloticus) juvenile. African Journal of Agricultural Research, 3, 825-834.
Baskaran, P. (1991). Aspects of the metabolism of Mytilus edulis L. during starvation. Netherlands Journal of Sea Research, 7:399-410.
Bhatkar, N. and Dhande, R. R. (2000). Furadan induced haematological changes in the freshwater fish (Labeo rohita). Environmental Monitoring and Assessment, 10(3):193-196.
Bojarski B., Ludwikowska A., Kurek A., Pawlak K., Tombarkiewicz B., Lutnicka H. (2015). Hematological alterations in common carp (Cyprinus carpio L.) exposed to herbicides: pendimethalin and ethofumesate tested separately and in mixture. Folia Biologica (Kraków) 63: 167-174.
Engel LS, O’Meara ES, Schwartz SM. (2000). Maternal occupation in agriculture and risk of limb defects in Washington State,
Fafioye 0. 0., Adebisi A. A. and Fagade S. 0 (2004). Toxicity of Parkia biglobosa and Raphia vinfera extracts on Clarias gariepinus juveniles, African Journal of Biotechnology. 3(1 1):627-630
Heath, A. G. (1995). Water Pollution and Fish Physiology, second ed. Lewis Publishers, CRC Boca Raton Florida.; 359.
Joshi PK, Bose M, Harish D (2002). Changes in certain hematological parameters in a siluroid cat fish, Clarias baterchus (L.) exposed to cadmium chloride. Pollut. Res. 21 (2): 129-131.
Karuppasamy, R, Subathra, S. And puvaneswari, S.(2005). Haematological responses to sublethal concentrations of cadmium in air breathing fish, channa punctatus. Journal of environmenta1l9 biol9ogy s26:a1s2d3-8
Kreutz L. C., Barcellos L. J. G., DE Faria Valle S., DE Oliveira Silva T., Anziliero D., Dos Santos E. D., Pivato M., Zanatta R. (2011). Altered hematological parameters and Immunological parameters in silver catfish (Rhamdia quelen) following short term exposure to sublethal concentration of glyphosate. Fish Shell fish Immunol. 30: 51-57.
Masopust J. (2000) Clinical biochemistry. Karolinum, Praha.
Nabela I. El-Sharkawy, Rasha M. Reda and Iman E. El-Araby (2011). Assessment of Stomp® (Pendimethalin) toxicity on Oreochromis niloticus Journal of American Science, 2011; 7(10) http://www.americanscience.org
Ojutiku, R. O, F. P Asuwaju, I. O Ayanda, R. A Obande and O. O Agbelege (2013) Effect of Acute Toxicity of Cypermethrin on Some biochemical Parameters of Juveniles of Claria Gariepinus. (Burchell, 1822) International Journal of Engineering Science Invention ISSN (Online): 2319 – 6734, ISSN (Print): 2319 – 6726 www.ijesi.org Volume 2 Issue 3 ǁ March. 2013 ǁ PP. 01-07
Omoregie, E. (1998). Changes in haematology of the Nile Tilapia, Oreochromis niloticus (Trewavas) under the effect of crude oil. Acta Hydrobiologica, 40:284-292.
Onouha GC, Vkagwu J I. (2007) Acute Toxicity of Gramoxone (Paraquat-dichloride) to the fresh water cat fish Clarias gariepinus (Burch) post fingerlings. Nigerian Journal of Fisheries 2007; 4(2):105-115.
Parikh. P. H. and Upadhyay A. A. (2014) Pyrazosulfuron –ethyl induced alteration in haematology and blood biochemistry of Oreochromis mossambicus; acute study. International journal of science and technology 2014, Vol. 18(3), 1245-1253. www.experimentjournal.com
Velisek J, Stara A, Svobodova Z. (2011) The effects of pyrethroid and Triazine pesticides on fish physiology. Pesticides in the Modern World - Pests Control and Pesticides Exposure and toxicity Assessment. Dr. Margarita Stoytcheva (Ed.) ISBN: 978-953-307-457-3;
Wegwu, M. O and Omeodu, S. I (2010). Evaluation of selected Biochemical Indices in Clarias gariepinusExposed to Aqueous Extract of Nigrian Crude oil (Bonny Light). Journal of Applied Sci. Environ. Manage. Vol 14 (1) 77-81.
Wepener, V., Vanvuren, J. H. and Du Preez, H. H. (1992). The effect of hexavalent chromium at different pH values on the haematology of Tilapia sparmani (Cichlidae). Comparative Biochemistry and Physiology, 101(2):275-381.
Yaji, A. J, Auta. J, Oniye, S. J, Adakole J. A, and Usman, J. I (2011) Effects of cypermethrin on Behaviour and biochemical indices of Freshwater fish Oreochromis niloticus. Electronic journal of Environment, Agriculture and food chemistry.
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