Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq
American Journal of BioScience
Volume 1, Issue 1, May 2013, Pages: 1-5
Received: Apr. 14, 2013; Published: May 2, 2013
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Authors
Abdulsadah A. Rahi, College of Science/Wasit University, Iraq
Sundus Nsaif, College of Medicine/Kufa University, Iraq
Jaafar J. Hassoni, College of Medicine/Wasit University, Iraq
Magda A. Ali, College of Medicine/Wasit University, Iraq, TUMS-IC
Hassan A. Hamza, Al-Sadir Hospital/Ministry of Health, Iraq
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Abstract
Sixty -four specimens collected passively from patients with cutaneous ulcers suggestive of leishmaniasis attending hospital and health centres in all parts of Iraq for diagnosis were included in the study. The present study was conducted from June to December 2012 in Al-Karamah Teaching Hospital in Wasit Province, Iraq. Dermal scrapings were analysed both by parasitological (examination of Giemsa-stained smears and in vitro cultivation) methods and by a genus-specific PCR detection. Microscopy revealed amastigotes in 38 samples (59.4%) and in vitro cultivation gave positive results in 19 cases (29.7%), whereas PCR detected Leishmania in 44 samples (68.75 %). There are two forms of Cutaneous leishmaniasis (CL) in Iraq are caused by two species of Leishmania . Of 44 well-documented cases in our study ; 28 (63.6 %) were caused byLeishmania major and 16 (36.4 %) by Leishmania tropica.
Keywords
Cutaneous Leishmaniasis, PCR, Diagnosis
To cite this article
Abdulsadah A. Rahi, Sundus Nsaif, Jaafar J. Hassoni, Magda A. Ali, Hassan A. Hamza, Comparison of Diagnostic Methods inCutaneous Leishmaniasis in Iraq, American Journal of BioScience. Vol. 1, No. 1, 2013, pp. 1-5. doi: 10.11648/j.ajbio.20130101.11
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