Opportunistic infections including urinary tract infections (UTIs) are among the predominant cause of morbidity and mortality among HIV infected patients. Persons living with HIV/AIDS (PLWHA) are prone to infection from non-pathogenic microbes in the environment than normal individuals; and this development has been greatly attributed to the weakened immune system of HIV infected patients which makes it difficult to protect the body against invading commensal organisms. In this study, midstream urine (MSU) samples from HIV infected patients who attended the Federal Teaching Hospital Abakaliki (FETHA), Ebonyi State, Nigeria for routine antiretroviral therapy were evaluated by microbiological analysis for uropathogens. Antibiogram was also investigated on all isolated uropathogens by the Kirby-Bauer disk diffusion method. The uropathogens isolated from the MSU of HIV infected patients in this study were identified as: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Proteus mirabilis. S. aureus was the most prevalent isolated organism (n=34). This was followed by K. pneumoniae (n=17), E. coli (16) and P. mirabilis (n=8). All uropathogens produced varying rates of susceptibility and resistance patterns to the tested antibiotics. High sensitivity was observed with gentamicin, ciprofloxacin, ampicillin and amoxycillin-clavulanic acid. Cefotaxime, ceftazidime, cefazolin, ceftriaxone, cefepime, and cefoxitin (which are all 3rd-generation β-lactams) showed less efficacy against the uropathogens. This study draws attention to the increasing rate of UTIs amongst HIV infected patients in Abakaliki metropolis, Ebonyi State of Nigeria, and the resistance of uropathogens to some available antibiotics. Therefore, there is need to checkmate the menace through proper detection and treatment of affected individuals in order to improve the health status of PLWHA in this environment.
| Published in | American Journal of BioScience (Volume 1, Issue 3) |
| DOI | 10.11648/j.ajbio.20130103.11 |
| Page(s) | 50-53 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
HIV/AIDS, Opportunistic Infections, Uropathogens, Resistance, Nigeria
| [1] | Kathleen A.H (2008). Natural Approaches to Prevention and Treatment of Infections of the Lower Urinary Tract. Alternative Medicine Review, 13(3):227-244. |
| [2] | Krane N.K and Hamrahian M (2007). Pregnancy: Kidney Diseases and Hypertension. American Journal of Kidney Diseases, 49(2):336-345. |
| [3] | Okonko I.O., Ijandipe L.A., Donbraye-Emmanuel O.B., Ejembi J., Udeze A.O., Egun O.C., Fowotade A and Nkang A.O (2010). Detection of Urinary Tract Infection (UTI) among pregnant women in Oluyoro Catholic Hospital, Ibadan, South-Western Nigeria. Malaysian Journal of Microbiology, 6(1):16-24. |
| [4] | Boiley, M. C., Baggaley, R. F., Wang, L., Masse, B., White,R.G., Hayes and Alary.M.(2009). Heterosexual risk of HIV-1 infection per sexual act: systemic review and meta-analysis of observational study. The lancet infectious diseases. 9(2):118-29. |
| [5] | Willey, J.M., Sherwood, L. M., and Woolvertoon, C.J. (2008). Prescott, Harley and Klein’s Microbiology,7th ed, McGraw Hills companies, New York,323,925-31. |
| [6] | Awolude O.A, Adesina O.A, OladokunA, Mutiu W.B and Adewole I.F (2010). Asymptomatic bacteriuria among HIV positive pregnant women. Virulence, 1(3):130-133. |
| [7] | Mazulli, T., Skulnick , M., Small, G., Marshal, W., Hoban, D.J., Zhnel ,G.G., Finn, S. , and Low De (2001). Susceptibility of community gram negative urinary tract isolates to methicillin and other oral agents. Canadian journal of infectious diseases. 12(5):289-292. |
| [8] | Ejikeugwu P.C , Nkechukwu M.I., Ugwu C.M., Iroha I.R and Esimone C.O (2012). Extended-spectrum β-lactamase-producing Escherichia coli isolates from suspected community acquired urinary tract infections. European Journal of Scientific Research. 84(4):565-571. |
| [9] | Muratani, T and Matsumoto.T. (2004). Bacterial resistance to antimicrobials in urinary isolates. Int journal of antimicrobial agents.24:28-31. |
| [10] | Petrosilla, N ., Martini, L., Raffaele, B., Nicastri, F., Bordi, E and Alba, L.(2002) . Urinary tract infection in HIV- infected patients. International conference on AIDS. 9:7-14. |
| [11] | Mahamat, A., lavigne, J.P .,Fabbro-perray, P., Kinowski, J.M., Daures, J.P and Sotto, A. (2005). Evolution of fluroquinolones resistance among Escherichia coli urinary tract isolates from a French university hospital: application of the dynamic regression model. Clin microb Infect., 11:301-306 |
| [12] | Cheesbrough, M (2002). District Laboratory Practice in Tropical Countries. Part 2. Cambridge University Press. Pp. 54-59. |
| [13] | Clinical Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; 15thInformational Supplement 2005; (M100-S15). |
| [14] | Omoregie, R. and Eghafona, N.O (2009). Urinary tract infection among asymptomatic HIV patients in Benin City, Nigeria. British journal biomed science.,66(4):194-199. |
| [15] | Bigwan E. I and Wakjissa F. D (2013). Prevalence of Urinary Tract Infections among HIV Patients Attending a Non- Governmental Health Facility in Jos, Plateau State, Nigeria. International Journal of Biomedical and Advance Research., 04(08):528-533. |
| [16] | Iweriebor B. C, Obi C. L, Akinyemi O, Ramalivhana N. J, Hattori T and Okoh A. I (2012). Uropathogens isolated from HIV-infected patients from Limpopo Province, South Africa. African Journal of Biotechnology., 11(46):10598-10604. |
| [17] | Hasan,A.S.,Nair, D.,Kaur, J.,Baweja, G., Deb, M and Aggarwal, P.(2007). Resistance pattern of urinary isolates in tertiary Indian hospital. J. Ayub Med. College abbottabab. 19(1):39-41. |
APA Style
Iroha Ifeanyichukwu, Nwakeze Emmanuel, Ejikeugwu Chika, Oji Anthonia, Udu-Ibiam Esther, et al. (2013). Frequency and Antibiogram of Uropathogens Isolated from Urine Samples of HIV Infected Patients on Antiretroviral Therapy. American Journal of BioScience, 1(3), 50-53. https://doi.org/10.11648/j.ajbio.20130103.11
ACS Style
Iroha Ifeanyichukwu; Nwakeze Emmanuel; Ejikeugwu Chika; Oji Anthonia; Udu-Ibiam Esther, et al. Frequency and Antibiogram of Uropathogens Isolated from Urine Samples of HIV Infected Patients on Antiretroviral Therapy. Am. J. BioScience 2013, 1(3), 50-53. doi: 10.11648/j.ajbio.20130103.11
AMA Style
Iroha Ifeanyichukwu, Nwakeze Emmanuel, Ejikeugwu Chika, Oji Anthonia, Udu-Ibiam Esther, et al. Frequency and Antibiogram of Uropathogens Isolated from Urine Samples of HIV Infected Patients on Antiretroviral Therapy. Am J BioScience. 2013;1(3):50-53. doi: 10.11648/j.ajbio.20130103.11
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Download@article{10.11648/j.ajbio.20130103.11,
author = {Iroha Ifeanyichukwu and Nwakeze Emmanuel and Ejikeugwu Chika and Oji Anthonia and Udu-Ibiam Esther and Afiukwa Ngozi and Ngwu Justina},
title = {Frequency and Antibiogram of Uropathogens Isolated from Urine Samples of HIV Infected Patients on Antiretroviral Therapy},
journal = {American Journal of BioScience},
volume = {1},
number = {3},
pages = {50-53},
doi = {10.11648/j.ajbio.20130103.11},
url = {https://doi.org/10.11648/j.ajbio.20130103.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20130103.11},
abstract = {Opportunistic infections including urinary tract infections (UTIs) are among the predominant cause of morbidity and mortality among HIV infected patients. Persons living with HIV/AIDS (PLWHA) are prone to infection from non-pathogenic microbes in the environment than normal individuals; and this development has been greatly attributed to the weakened immune system of HIV infected patients which makes it difficult to protect the body against invading commensal organisms. In this study, midstream urine (MSU) samples from HIV infected patients who attended the Federal Teaching Hospital Abakaliki (FETHA), Ebonyi State, Nigeria for routine antiretroviral therapy were evaluated by microbiological analysis for uropathogens. Antibiogram was also investigated on all isolated uropathogens by the Kirby-Bauer disk diffusion method. The uropathogens isolated from the MSU of HIV infected patients in this study were identified as: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Proteus mirabilis. S. aureus was the most prevalent isolated organism (n=34). This was followed by K. pneumoniae (n=17), E. coli (16) and P. mirabilis (n=8). All uropathogens produced varying rates of susceptibility and resistance patterns to the tested antibiotics. High sensitivity was observed with gentamicin, ciprofloxacin, ampicillin and amoxycillin-clavulanic acid. Cefotaxime, ceftazidime, cefazolin, ceftriaxone, cefepime, and cefoxitin (which are all 3rd-generation β-lactams) showed less efficacy against the uropathogens. This study draws attention to the increasing rate of UTIs amongst HIV infected patients in Abakaliki metropolis, Ebonyi State of Nigeria, and the resistance of uropathogens to some available antibiotics. Therefore, there is need to checkmate the menace through proper detection and treatment of affected individuals in order to improve the health status of PLWHA in this environment.},
year = {2013}
}
TY - JOUR T1 - Frequency and Antibiogram of Uropathogens Isolated from Urine Samples of HIV Infected Patients on Antiretroviral Therapy AU - Iroha Ifeanyichukwu AU - Nwakeze Emmanuel AU - Ejikeugwu Chika AU - Oji Anthonia AU - Udu-Ibiam Esther AU - Afiukwa Ngozi AU - Ngwu Justina Y1 - 2013/10/30 PY - 2013 N1 - https://doi.org/10.11648/j.ajbio.20130103.11 DO - 10.11648/j.ajbio.20130103.11 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 50 EP - 53 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20130103.11 AB - Opportunistic infections including urinary tract infections (UTIs) are among the predominant cause of morbidity and mortality among HIV infected patients. Persons living with HIV/AIDS (PLWHA) are prone to infection from non-pathogenic microbes in the environment than normal individuals; and this development has been greatly attributed to the weakened immune system of HIV infected patients which makes it difficult to protect the body against invading commensal organisms. In this study, midstream urine (MSU) samples from HIV infected patients who attended the Federal Teaching Hospital Abakaliki (FETHA), Ebonyi State, Nigeria for routine antiretroviral therapy were evaluated by microbiological analysis for uropathogens. Antibiogram was also investigated on all isolated uropathogens by the Kirby-Bauer disk diffusion method. The uropathogens isolated from the MSU of HIV infected patients in this study were identified as: Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Proteus mirabilis. S. aureus was the most prevalent isolated organism (n=34). This was followed by K. pneumoniae (n=17), E. coli (16) and P. mirabilis (n=8). All uropathogens produced varying rates of susceptibility and resistance patterns to the tested antibiotics. High sensitivity was observed with gentamicin, ciprofloxacin, ampicillin and amoxycillin-clavulanic acid. Cefotaxime, ceftazidime, cefazolin, ceftriaxone, cefepime, and cefoxitin (which are all 3rd-generation β-lactams) showed less efficacy against the uropathogens. This study draws attention to the increasing rate of UTIs amongst HIV infected patients in Abakaliki metropolis, Ebonyi State of Nigeria, and the resistance of uropathogens to some available antibiotics. Therefore, there is need to checkmate the menace through proper detection and treatment of affected individuals in order to improve the health status of PLWHA in this environment. VL - 1 IS - 3 ER -
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