Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor
International Journal of Mechanical Engineering and Applications
Volume 4, Issue 2, April 2016, Pages: 81-87
Received: May 7, 2016; Published: May 9, 2016
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Authors
Xiarong Zhou, Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Shangquan Wu, Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Hong Liu, Department of Chemical Physics, University of Science and Technology of China, Hefei, People’s Republic of China
Xiaoping Wu, Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Qingchuan Zhang, Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
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Abstract
As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.
Keywords
Microcantilever, Nanomechanical Sensor, Aflatoxin B1, Label-Free, Surface Stress
To cite this article
Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang, Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor, International Journal of Mechanical Engineering and Applications. Vol. 4, No. 2, 2016, pp. 81-87. doi: 10.11648/j.ijmea.20160402.17
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