Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip
International Journal of Biomedical Materials Research
Volume 4, Issue 3, December 2016, Pages: 18-26
Received: Oct. 8, 2016; Accepted: Oct. 17, 2016; Published: Nov. 10, 2016
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Authors
Lamia'a Sobhi. Saqer, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Haneen Ahmad. Khammash, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Eman Loai. Shurrab, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Mariam Nahed. Aabed, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
Rana Afif. El-Malakh, Medical Sciences Department, University College of Science and Technology, Gaza Strip, Palestine
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Abstract
The insertion\ deletion (ID) polymorphism in intron 16 of the ACE gene is a marker for a functional polymorphism, and it is also reported to influence levels of ACE in healthy subjects. ACE ID polymorphism is associated with an elevated risk of thrombosis and may be causally associated with coronary heart disease (CHD). To investigate the association between ACE gene polymorphism and CHD in the Gaza strip population, we conducted case-control study included 260 samples comprised 160 CHD patients and 100 control subjects. Questionnaire interview was applied. Blood samples were collected in EDTA tube for DNA extraction. Polymerase Chain Reaction (PCR) used to detect ACE ID polymorphism. There was significant association between CHD and age, physical activity, education level, occupation, and family history of CHD. No significant association was observed between CHD and gender and smoking. The genotype frequencies were: ID (36.9%, 42%), DD (53.8%, 54%) and II (9.4%, 4%) in case and control groups respectively. The ACE ID allele frequencies were: I (27%, 25%) and D (72.2%, 75%) in case and control group respectively. The D allele was the most frequent allele in both groups. No association between ACE ID polymorphism and gender. The DD genotype was the most frequent in both study groups. There was no statistically significant association between the ACE ID polymorphism and CHD in Gaza strip. The results showed that there was no significant association between the ACE ID gene polymorphism and CHD in Gaza strip.
Keywords
Angiotensin Converting Enzyme Gene, Coronary Heart Disease, Polymerase Chain Reaction, Polymorphism
To cite this article
Lamia'a Sobhi. Saqer, Haneen Ahmad. Khammash, Eman Loai. Shurrab, Mariam Nahed. Aabed, Rana Afif. El-Malakh, Association Between Angiotensin Converting Enzyme Gene Insertion\Deletion Polymorphism and Coronary Heart Disease in Gaza Strip, International Journal of Biomedical Materials Research. Vol. 4, No. 3, 2016, pp. 18-26. doi: 10.11648/j.ijbmr.20160403.12
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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